The PD-type iterative learning control design of a class of affine nonlinear time-delay systems with external disturbances is considered. Sufficient conditions guaranteeing the convergence of the n-norm of the trackin...The PD-type iterative learning control design of a class of affine nonlinear time-delay systems with external disturbances is considered. Sufficient conditions guaranteeing the convergence of the n-norm of the tracking error are derived. It is shown that the system outputs can be guaranteed to converge to desired trajectories in the absence of external disturbances and output measurement noises. And in the presence of state disturbances and measurement noises, the tracking error will be bounded uniformly. A numerical simulation example is presented to validate the effectiveness of the proposed scheme.展开更多
Cancer stands as one of the predominant causes of mortality globally, necessitating ongoing efforts to develop innovative therapeutics. Historically, natural products have been foundational in the quest for anticancer...Cancer stands as one of the predominant causes of mortality globally, necessitating ongoing efforts to develop innovative therapeutics. Historically, natural products have been foundational in the quest for anticancer agents. Bulbocodin D (BD) and Bulbocodin C (BC), two bibenzyls derived from Pleione bulbocodioides (Franch.) Rolfe, have demonstrated notable in vitro anticancer activity. In human lung cancer A549 cells, the IC50s for BD and BC were 11.63 and 11.71 μmol·L^(−1), respectively. BD triggered apoptosis, as evidenced by an upsurge in Annexin V-positive cells and elevated protein expression of cleaved-PARP in cancer cells. Furthermore, BD and BC markedly inhibited the migratory and invasive potentials of A549 cells. The altered genes identified through RNA-sequencing analysis were integrated into the CMap dataset, suggesting BD’s role as a potential signal transducer and activator of transcription 3 (STAT3) inhibitor. SwissDock and MOE analyses further revealed that both BD and BC exhibited a commendable binding affinity with STAT3. Additionally, a surface plasmon resonance assay confirmed the direct binding affinity between these compounds and STAT3. Notably, treatment with either BD or BC led to a significant reduction in p-STAT3 (Tyr 705) protein levels, regardless of interleukin-6 stimulation in A549 cells. In addition, the extracellular signal-regulated kinase (ERK) was activated after BD or BC treatment. An enhancement in cancer cell mortality was observed upon combined treatment of BD and U0126, the MEK1/2 inhibitor. In conclusion, BD and BC emerge as promising novel STAT3 inhibitors with potential implications in cancer therapy.展开更多
基金This project was supported by the National Natural Science Foundation of China (60074001) and the Natural ScienceFoundation of Shandong Province (Y2000G02)
文摘The PD-type iterative learning control design of a class of affine nonlinear time-delay systems with external disturbances is considered. Sufficient conditions guaranteeing the convergence of the n-norm of the tracking error are derived. It is shown that the system outputs can be guaranteed to converge to desired trajectories in the absence of external disturbances and output measurement noises. And in the presence of state disturbances and measurement noises, the tracking error will be bounded uniformly. A numerical simulation example is presented to validate the effectiveness of the proposed scheme.
基金financially supported by the National Natural Science Foundation of China(Nos.50801039and11074227)Natural Science Foundation of Zhejiang Province(No.Y4090022),Ningbo City(No.2012A610054)K.C.Wong Magna Fund in Ningbo University
基金supported by the Science and Technology Development Fund,Macao SAR(File no.0053-2021-AGJ)the Joint Foundation of Guangdong and Macao for Science and Technology Innovation(2022A0505020024)+3 种基金the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund(Guangdong-Hong Kong-Macao Joint Lab,File No.:2020B1212030006)the Internal Research Grant of the State Key Laboratory of Quality Research in Chinese Medicine,University of Macao(File No.:SKL-QRCM-IRG2023-011)the Natural Science Foundation of Fujian Province(2022J01244)the Outstanding-Young Scientific Research Talents Program of Fujian Agriculture and Forestry University(XJQ202103)。
文摘Cancer stands as one of the predominant causes of mortality globally, necessitating ongoing efforts to develop innovative therapeutics. Historically, natural products have been foundational in the quest for anticancer agents. Bulbocodin D (BD) and Bulbocodin C (BC), two bibenzyls derived from Pleione bulbocodioides (Franch.) Rolfe, have demonstrated notable in vitro anticancer activity. In human lung cancer A549 cells, the IC50s for BD and BC were 11.63 and 11.71 μmol·L^(−1), respectively. BD triggered apoptosis, as evidenced by an upsurge in Annexin V-positive cells and elevated protein expression of cleaved-PARP in cancer cells. Furthermore, BD and BC markedly inhibited the migratory and invasive potentials of A549 cells. The altered genes identified through RNA-sequencing analysis were integrated into the CMap dataset, suggesting BD’s role as a potential signal transducer and activator of transcription 3 (STAT3) inhibitor. SwissDock and MOE analyses further revealed that both BD and BC exhibited a commendable binding affinity with STAT3. Additionally, a surface plasmon resonance assay confirmed the direct binding affinity between these compounds and STAT3. Notably, treatment with either BD or BC led to a significant reduction in p-STAT3 (Tyr 705) protein levels, regardless of interleukin-6 stimulation in A549 cells. In addition, the extracellular signal-regulated kinase (ERK) was activated after BD or BC treatment. An enhancement in cancer cell mortality was observed upon combined treatment of BD and U0126, the MEK1/2 inhibitor. In conclusion, BD and BC emerge as promising novel STAT3 inhibitors with potential implications in cancer therapy.
