With proteomic analysis,we identified 379 egg surface proteins from an endoparasitoid,Cotesia chilonis.Proteins containing conserved enzymatic domains constitute a large proportion of egg surface components.Some prote...With proteomic analysis,we identified 379 egg surface proteins from an endoparasitoid,Cotesia chilonis.Proteins containing conserved enzymatic domains constitute a large proportion of egg surface components.Some proteins,such as superoxidase dismutase,homolog of C.rubecula 32-kDa protein,and immunoevasive protein-2A,are classical parasitism factors that have known functions in host immunity regulation.Melanization assays revealed that a novel egg surface protein,C.chilonis egg surface serpin domain-containing protein had the same function as a C.chilonis venom serpin,as both suppressed host melanization in a dose-dependent manner.C.chilonis egg surface serpin domain-containing protein is mainly transcribed in C.chilonis oocytes with follicular cells,and it is located on both the anterior and posterior sides of the mature egg surface.Additionally,we used LC-MS/MS to identify 586 binding proteins sourced from C.suppressalis plasma located on the eggshell surface of C.chilonis,which included some immunity-related proteins.These results not only indicate that C.chilonis uses its egg surface proteins to reduce the immune response of its host but also imply that endoparasitoid egg surface proteins might be a new parasitism factor involved in host immune regulation.展开更多
基金Financial support was provided by National Natural Science Foundation of China(Grant No.31801796,http:/www.nsfc.gov.cn/)Major International(Regional)Joint Research Project of National Natural Science Foundation(Grant No.31620103915,http://www.nsfc.gov.cn/)+3 种基金Key Program of National Natural Science Foundation of China(NSFC)(Grant no.31830074,http:/www.nsfc.gow.cn/)National Key R&D Program of China(No.2017YFD0200400)The Program for Chi-nese Innovation Team in Key Areas of Science and Technology of Ministry of Science and Technology ofthe People's Republic of China(2016RA4008)Program for Chinese Outstanding Talents in Agricultural Scientifie Research of Ministry of Agriculture and Rural Affairs of the People's Republic of China.
文摘With proteomic analysis,we identified 379 egg surface proteins from an endoparasitoid,Cotesia chilonis.Proteins containing conserved enzymatic domains constitute a large proportion of egg surface components.Some proteins,such as superoxidase dismutase,homolog of C.rubecula 32-kDa protein,and immunoevasive protein-2A,are classical parasitism factors that have known functions in host immunity regulation.Melanization assays revealed that a novel egg surface protein,C.chilonis egg surface serpin domain-containing protein had the same function as a C.chilonis venom serpin,as both suppressed host melanization in a dose-dependent manner.C.chilonis egg surface serpin domain-containing protein is mainly transcribed in C.chilonis oocytes with follicular cells,and it is located on both the anterior and posterior sides of the mature egg surface.Additionally,we used LC-MS/MS to identify 586 binding proteins sourced from C.suppressalis plasma located on the eggshell surface of C.chilonis,which included some immunity-related proteins.These results not only indicate that C.chilonis uses its egg surface proteins to reduce the immune response of its host but also imply that endoparasitoid egg surface proteins might be a new parasitism factor involved in host immune regulation.
