Physicochemical Properties and Eating Quality of Lamp Meat of Dazu Black Goats

The routine nutrients, mineral content, amino acid content and fatty acid content as well as the physicochemical properties and the heavy metal and pesticide residues of Iongissimus dorsi muscles in confined Dazu black goats at the age of 6 months were determined. The results showed that the lamb meat of Dazu black goats was dch in nutrients, fresh, tender, palatable and fine in texture as well as non-hazardous in quality. Thus, the meat of Dazu black goat is a kind of healthy and nutritious food with rich nutrients, and it has better prospects of exploitation and utilization in the food industry.

Cold plasma promotes Sertoli cell proliferation via AMPK–mTOR signaling pathway

This study investigated cold plasmas for multiple biological applications. Our previous work has found dielectric barrier discharge plasma improves chicken sperm quality. The number of Sertoli cells(SCs) decides spermatogenesis. However, whether cold plasma can regulate SC proliferation remains unclear. This study explored the effects of cold plasma on immature chicken SC proliferation and the regulation mechanism. Results showed that cold plasma exposure at 2.4 W for 30 s twice with an interval of 6 h produced(P<0.05) the maximum SC viability, cell growth, and cell cycle progression. SC proliferation-promoting effect of cold plasma treatment was regulated by increasing(P<0.05) the adenosine triphosphate production and the respiratory enzyme activity in the mitochondria. This process was potentially mediated by the adenosine monophosphate-activated protein kinase(AMPK)–mammalian target of rapamycin(m TOR) signaling pathway, which was regulated by the micro RNA(mi RNA) targeting regulation directly and by the intracellular reactive oxygen species homeostasis indirectly. The cold plasma treatment increased(P<0.01) the mi R-7450-5 p expression and led to a decreased(P<0.01) AMPKα1 level. On the other hand, mi R-100-5 p expression was reduced(P<0.05) and led to an increased(P<0.05) m TOR level in SCs. A single-stranded synthetic mi R-7450-5 p antagomir and a double-stranded synthetic mi R-100-5 p agomir reduced(P<0.05) the SC proliferation. However, this could be ameliorated(P<0.05) by the cold plasma treatment. Our findings suggest that appropriate cold plasma treatment provides a safe strategy to improve SC proliferation, which is beneficial to elevating male chicken reproductive capacity.