Objective To understand the infected strains and prevalence of brucellosis in occupational population in Tacheng and Kashgar regions,Xinjiang Uygur Autonomous Region.Methods In September 2015,blood samples from occupa...Objective To understand the infected strains and prevalence of brucellosis in occupational population in Tacheng and Kashgar regions,Xinjiang Uygur Autonomous Region.Methods In September 2015,blood samples from occupational population(including herders,semi-agricultural and semi-pastoral,veterinarians)and non-occupational population(including students and cadres)were collected to detect Brucella-specific antibody and bacterial nucleic acids by rose bengal plate test(RBPT),serological standard tube agglutination test(SAT)and PCR methods,respectively.The positive products of PCR were sent to Shanghai Sangon Biotechnology Co.,LTD.Then the sequence results were retrieved online using the basic alignment search tool(BLAST)in GenBank web page and uploaded to NCBI(National Center for Biotechnology Information).Results A total of 546 blood samples were tested,including 300 males,aged(55±15)years old,and 246 females,aged(54±12)years old.The positive rates were 17.58%(96/546)and 6.78%(37/546)in 546 blood samples by serological method and genetic markers targeting omp22 and omp2,respectively.The positive rates were statistically significant(χ^2=29.8,P<0.05).Additionally,based on BLAST analysis of outer membrane protein omp22 and omp2 genes,the positive products were identified as Brucella abortus,and the sequence similarity was 100.00%(253/253,863/863 bp)to Brucella abortus strain Wisconsin genome assembly,chromosome(LT651712).Conclusion Brucellosis has a high infection rate in the occupational population of some animal husbandry-based groups in Xinjiang.The infection strain is abortive species Brucella,and health education for the occupational population and prevention of brucellosis should be strengthened to reduce the infection rate.展开更多
文摘Objective To understand the infected strains and prevalence of brucellosis in occupational population in Tacheng and Kashgar regions,Xinjiang Uygur Autonomous Region.Methods In September 2015,blood samples from occupational population(including herders,semi-agricultural and semi-pastoral,veterinarians)and non-occupational population(including students and cadres)were collected to detect Brucella-specific antibody and bacterial nucleic acids by rose bengal plate test(RBPT),serological standard tube agglutination test(SAT)and PCR methods,respectively.The positive products of PCR were sent to Shanghai Sangon Biotechnology Co.,LTD.Then the sequence results were retrieved online using the basic alignment search tool(BLAST)in GenBank web page and uploaded to NCBI(National Center for Biotechnology Information).Results A total of 546 blood samples were tested,including 300 males,aged(55±15)years old,and 246 females,aged(54±12)years old.The positive rates were 17.58%(96/546)and 6.78%(37/546)in 546 blood samples by serological method and genetic markers targeting omp22 and omp2,respectively.The positive rates were statistically significant(χ^2=29.8,P<0.05).Additionally,based on BLAST analysis of outer membrane protein omp22 and omp2 genes,the positive products were identified as Brucella abortus,and the sequence similarity was 100.00%(253/253,863/863 bp)to Brucella abortus strain Wisconsin genome assembly,chromosome(LT651712).Conclusion Brucellosis has a high infection rate in the occupational population of some animal husbandry-based groups in Xinjiang.The infection strain is abortive species Brucella,and health education for the occupational population and prevention of brucellosis should be strengthened to reduce the infection rate.
