Clinical observation of electroacupuncture"Tongmin Point"combined with heat-sensitive moxibustion on knee osteoarthritis and its influence on blood lipid level and ADAMTS-4

Objective:To observe the clinical effect of electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion on knee osteoarthritis and its effect on blood lipid level,polyprotein polysaccharide enzyme-4(a disintegrin and metalloprotease with thrombospondin motifs,ADAMTS-4,matrix metalloproteinase-3(matrix metalloproteinase-3,);and MMPs-3)Impact.Methods:80 patients were randomly divided into two groups,40 cases per group,Treatment and control groups,The treatment group was given electroacupuncture combined with heat-sensitive moxibustion,Six times a week,Continuous treatment for 4 weeks;The control group was given intraarticular sodium hyaluronate,Once a week,continuous treatment for 4 weeks.Results:During the treatment period,Visual analogue scale visual analogue scale,VAS)show a downward trend,Intra-group comparisons,Differences were statistically significant(P<0.05);After 2 weeks of treatment,4 weeks of treatment and 2 weeks of follow-up,All the scores in the treatment group were lower than those in the control group(P<0.05).After treatment in two groups(The Western Ontario and Mc Master Universities Osteoarthritis Index,)of osteoarthritis index,University of Western Ontario WOMAC)The scores of each item decreased significantly,treatment group was better than the control group(P<0.05).After four weeks of treatment,(Total cholesterol,)Total cholesterol TC,triglycerides(Triglyceride,);and TG,LDL(Low density lipoprotein,);and LDL-C)A decrease in content,High density lipoprotein High density lipoprotein,HDL-C)Increased content,and the improvement was better than that of the control group(P<0.05).After treatment,the ADAMTS-4,MMPs-3 content decreased,Intergroup comparisons,Differences were statistically significant(P<0.05);The effective rate in the treatment group was 87.5%,The control rate was 60%,treatment group was more effective(P<0.05).Conclusions:Electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion can obviously improve the symptoms of knee osteoarthritis patients,Regulate blood lipids,High security,It is worth popularizing in clinic.

Effect of Hirudin on farnesol X receptor pathway during acute intrahepatic cholestasis

Objective:The purpose of this study is to explore the effect of Hirudin on the farnesoid X receptor(FXR)pathway during acute intrahepatic cholestasis in vivo and in vitro.Method:In vivo,sixty male Sprague-Dawley rats were randomly divided into six groups:regular group,model group,ursodeoxycholic acid(UDCA)group(60 mg/kg),hirudin treatment group(84 u/kg),hirudin treatment group(63 u/kg)and hirudin treatment group(42 u/kg).The male Sprague-Dawley rats of UDCA group were intragastrically administered with a corresponding concentration of 0.005 mL/g body weight for seven days,once a day;and the hirudin treatment group was injected subcutaneously with different concentrations of Hirudin for seven days,once a day;Except for the normal group,other groups of rats were given 100 mg/kg ANIT by gavage on the 5th day.The model was administered by gavage once a day for three days.In vitro,(Z)-Guggulsterone was used to stimulate the L02 cells(0.05μmol/ml),with or without different concentrations of Hirudin(2,4 and 8 u/ml)for 24 h.The liver tissue was examined by HE microscope and the pathological state of the rat liver was observed;FXR,Small heterodimeric chaperone receptor(SHP),uridine diphosphate glucuronide transfer 2B4(UGT2B4),bile salt output pump(BSEP)mRNA and protein expressions were tested by real-time fluorescent quantitative PCR and Western blot test.And immunohistochemistry(IHC)was used to analyze the expression of FXR.Results:Compared with the model group,the hirudin group can improve liver tissue damage,and promote FXR,SHP,BSEP and UGT2B4 proteins and mRNA expression in vivo and in vitro.Conclusion:Hirudin can alleviate intrahepatic cholestasis,reduce liver tissue damage.Hirudin can up-regulate the expression of FXR gene,promote the up-regulation of SHP,BSEP and UGT2B4 genes,and inhibit the cholestasis pathway to protect liver cells.The study may provide an effective drug for clinical treatment of intrahepatic cholestasis.