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Peptidase Activities of Tripeptidyl Peptidase Ⅰ (TPP Ⅰ): Exopeptidase and Endopeptidase
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作者 DU Pei-ge AN Li-ping +1 位作者 QIU Fang-ping LU Gang 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2006年第1期65-67,共3页
The defect of TPP Ⅰ causes a disease, late infantile neuronal ceroid lipofuscinosis(LINCL, CLN2). To investigate the bio-activity of tripeptidyl peptidase Ⅰ (TPP Ⅰ ) from rat kidneys, the effects of digestion o... The defect of TPP Ⅰ causes a disease, late infantile neuronal ceroid lipofuscinosis(LINCL, CLN2). To investigate the bio-activity of tripeptidyl peptidase Ⅰ (TPP Ⅰ ) from rat kidneys, the effects of digestion of angiotensin Ⅱ (Ang Ⅱ ) and a synthetic endo-type substrate( Gly^1-Lys-Pro^5-lie-Pro^5-Phe-Phe-Arg-Leu-Lys^10) via TPP I were ana- lyzed by HPLC and TOF-MS. The data suggest that the degradation rate of Ang I1 can reach 18. 2% by the rat TPP I and DRV(Asp-Arg-Val) can be released from N-termini of Ang Ⅱ within 16 h. In addition, the synthetic endotype substrate is cleaved at the same position between Phe6 and Phe^7. Accordingly, TPP Ⅰ shows two kinds of peptidase activities. One is a tripeptidyl peptidase activity and the other is a pepstatin insensitive carboxyl endopeptidase activity. Tripeptidyl peptidase activity and pepstatin insensitive carboxyl endopeptidase activity seem to be dual phases of one enzyme, TPP Ⅰ. 展开更多
关键词 Rat TPP Angiotensin Endo-type substrate Endopeptidase activity
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