A new fluorescent probe, NPQ-C, was synthesized. NPQ-C was based on the naphthalimide derivatives and exhibited high selectivity and sensitivity for Cr3＋ ions. As a Cr3＋-amplified fluorescent probe, Its fluorescence...A new fluorescent probe, NPQ-C, was synthesized. NPQ-C was based on the naphthalimide derivatives and exhibited high selectivity and sensitivity for Cr3＋ ions. As a Cr3＋-amplified fluorescent probe, Its fluorescence spectrum showed 5.5-fold enhancement in the intensity of the signal at 500 nm on binding with the Cr3＋. Based on the fluorescence titration spectra and Job＇s-plot analysis, binding mode of NPQ-C with Cr3＋ was proposed. Fluorescence intensity was linear with concentration of Cr3＋ cation in a range from 0 to 10 μmol·L-1. NPQ-C was also sensitive for Cr3＋. The detection limit was calculated to be 0.20 pmol.L-1 which indicated that NPQ-C was sensitive to Cr3＋.展开更多
A new copper and silver-mediated cyanation of aryl iodides with DDQ as a cyanide source is achieved, provid- ing nitriles with good yields. This new approach represents a safe method leading to aryl nitriles.
Natural and syntlietic nanopores are increasingly popular tools in biosensors. In this work, the DNA supersandwich structure, which was made from two specially designed probes has been used to be fabricated in solid n...Natural and syntlietic nanopores are increasingly popular tools in biosensors. In this work, the DNA supersandwich structure, which was made from two specially designed probes has been used to be fabricated in solid nanopores. Integrating the idea of affinity between streptavidin and biotin, the DNA supersandwich structure with biotins was successfully constructed for streptavidin detection, and the limitation of detection was found to be 10 fmol/L. This nanodevice allows specific, sensitive and versatile detection of diverse analytes with easy operations, thus we believe that it could be developed to detect some disease-related molecular targets and play a considerable role in biotechnology.展开更多
The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HC...The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the sensors.In this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and SSA.This review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions.展开更多
基金The authors gratefully acknowledge the National Nature Science Foundation of China，the support from the Fundamental Research Funds for the Central Universities,the Opening Foundation of Zhejiang Provincial Top Key Discipline,and Baihehua Group
文摘A new fluorescent probe, NPQ-C, was synthesized. NPQ-C was based on the naphthalimide derivatives and exhibited high selectivity and sensitivity for Cr3＋ ions. As a Cr3＋-amplified fluorescent probe, Its fluorescence spectrum showed 5.5-fold enhancement in the intensity of the signal at 500 nm on binding with the Cr3＋. Based on the fluorescence titration spectra and Job＇s-plot analysis, binding mode of NPQ-C with Cr3＋ was proposed. Fluorescence intensity was linear with concentration of Cr3＋ cation in a range from 0 to 10 μmol·L-1. NPQ-C was also sensitive for Cr3＋. The detection limit was calculated to be 0.20 pmol.L-1 which indicated that NPQ-C was sensitive to Cr3＋.
基金We thank the National Natural Science Foundation of China （No. 21272178）, the Natural Science Founda- tion of Zhejiang Province （Nos. R4110294 and LY12B02010）.
文摘A new copper and silver-mediated cyanation of aryl iodides with DDQ as a cyanide source is achieved, provid- ing nitriles with good yields. This new approach represents a safe method leading to aryl nitriles.
基金Supported by the National Natural Science Foundation of China(No.21505101)the Natural Science Foundation of Zhejiang Province,China(No.LQ16B050003).
文摘Natural and syntlietic nanopores are increasingly popular tools in biosensors. In this work, the DNA supersandwich structure, which was made from two specially designed probes has been used to be fabricated in solid nanopores. Integrating the idea of affinity between streptavidin and biotin, the DNA supersandwich structure with biotins was successfully constructed for streptavidin detection, and the limitation of detection was found to be 10 fmol/L. This nanodevice allows specific, sensitive and versatile detection of diverse analytes with easy operations, thus we believe that it could be developed to detect some disease-related molecular targets and play a considerable role in biotechnology.
基金supported by the National Basic Research Program of China(2015CB932600,2013CB933000)the National Natural Science Foundation of China(21505101,21375042,21405054, 21404097)1000 Young Talent(to Fan Xia) and Zhejiang Provincial Natural Science Foundation of China(LQ16B050003)
文摘The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the sensors.In this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and SSA.This review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions.
