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Expression and Analysis of Microtus fortis against Schistosoma japonicum CD36 Gene
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作者 Yang Xiang Xue-Qin Zheng +6 位作者 Zhen Xiang Dong-Song Nie Yu Liu Xian-Lei Li Dian-Dian Lv Jun-Jian Hu Pei He 《Journal of Life Sciences》 2013年第8期791-795,共5页
The total RNA was extracted from Microtusfortis liver tissue which before being infected and after being infected 10 d and 15 d by the Schistosoma japonicum cercariae. Using Rattus norvegicus CD36 gene probe to hybrid... The total RNA was extracted from Microtusfortis liver tissue which before being infected and after being infected 10 d and 15 d by the Schistosoma japonicum cercariae. Using Rattus norvegicus CD36 gene probe to hybridize analysis of CD36 difference expression in the Microtus fortis liver tissues which were infected with Schistosorna japonicum before and after being infected. At the same time, the cDNA sequence and encoded amino acid sequence of the Rattus norvegicus CD36 gene and CD36 protein structural domains were analysized by using bioinformatics. The results showed that the CD36 expression levels in the liver tissue of Microtus fortis after being infected were significantly higher than before being infectied. The Rattus norvegicus CD36 cDNA sequence of a total length is 1625 bp and encoded 472 amino acid residues and Rattus norvegicus CD36 protein containing a CD36 superfamily domain. 展开更多
关键词 Microtusfortis Schistosomajaponicum CD36 EXPRESSION analysis.
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