A standardized extract of the plant Caralluma fimbriata (Slimaluma?) is widely used in the management of obesity but its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata ...A standardized extract of the plant Caralluma fimbriata (Slimaluma?) is widely used in the management of obesity but its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata extract (CFE) to modify pre-adipocyte cell division and thus the development of hyper-plastic obesity. Mouse 3T3-L1 pre-adipocyte cell line samples were treated with different concentrations of an extract of CFE standardized against its pregnane glycoside content. Plain medium formed the negative control and hydroxyurea was the positive control. The cells were counted at 12-hour intervals, and their viability tested using the MTT assay. The treated cells were subjected to direct and indirect immunofluorescent assays for cyclin D1. CFE inhibited 3T3-L1 cell growth in a dose and duration-dependent manner, with results comparable to those produced by hydroxyurea. The viability of CFE-treated cells was reduced. Direct and indirect immunofluorescent assays demonstrated that CFE inhibits import of cyclin D1into the nucleus. CFE appears to inhibit pre-adipocyte cell division by interfering with a mechanism preceding the import of cyclin D1-CDk4/6 complex into the nucleus during the early G1 phase of the cell cycle, suggesting that CFE has the potential to inhibit hyperplastic obesity.展开更多
Cattle are arguably the most important livestock species. Since the beginning of this century, the loss of genetic diversity within this species has been a major concern as this could have serious consequences on the ...Cattle are arguably the most important livestock species. Since the beginning of this century, the loss of genetic diversity within this species has been a major concern as this could have serious consequences on the ability of this species to respond to future production constraints. Genetic diversity has traditionally been accessed from pedigree, however, with the advances in molecular genetics new opportunities have emerged. We examined different methods for accessing genetic diversity and estimating genetic diversity parameters at the genomic level. We also examined criteria and strategies for the conservation of animal genetic resources. Finally, we examined genomic studies on some endangered cattle breeds.展开更多
文摘A standardized extract of the plant Caralluma fimbriata (Slimaluma?) is widely used in the management of obesity but its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata extract (CFE) to modify pre-adipocyte cell division and thus the development of hyper-plastic obesity. Mouse 3T3-L1 pre-adipocyte cell line samples were treated with different concentrations of an extract of CFE standardized against its pregnane glycoside content. Plain medium formed the negative control and hydroxyurea was the positive control. The cells were counted at 12-hour intervals, and their viability tested using the MTT assay. The treated cells were subjected to direct and indirect immunofluorescent assays for cyclin D1. CFE inhibited 3T3-L1 cell growth in a dose and duration-dependent manner, with results comparable to those produced by hydroxyurea. The viability of CFE-treated cells was reduced. Direct and indirect immunofluorescent assays demonstrated that CFE inhibits import of cyclin D1into the nucleus. CFE appears to inhibit pre-adipocyte cell division by interfering with a mechanism preceding the import of cyclin D1-CDk4/6 complex into the nucleus during the early G1 phase of the cell cycle, suggesting that CFE has the potential to inhibit hyperplastic obesity.
文摘Cattle are arguably the most important livestock species. Since the beginning of this century, the loss of genetic diversity within this species has been a major concern as this could have serious consequences on the ability of this species to respond to future production constraints. Genetic diversity has traditionally been accessed from pedigree, however, with the advances in molecular genetics new opportunities have emerged. We examined different methods for accessing genetic diversity and estimating genetic diversity parameters at the genomic level. We also examined criteria and strategies for the conservation of animal genetic resources. Finally, we examined genomic studies on some endangered cattle breeds.
