Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl...Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl-owth ,survival, and differentiation of specific nerve cell populations. Although animal tests and trials indicate that rhNGF could be ment for diabetic and HIV-related phase- Ⅱ clinical an effective treatneuropathies , a large-scale phase-Ⅲ clinical trial has failed to give similar result. NGF isolated from the mouse submaxillary gland has been widely used clinically in China for the treatment of peripheral neuropathy,展开更多
CXCR1 is a G-protein coupled receptor, transducing signals from chemokines, in particular the interleukin-8 （1L8） molecules. This study combines homology modeling and molecular dynamics simulation methods to study t...CXCR1 is a G-protein coupled receptor, transducing signals from chemokines, in particular the interleukin-8 （1L8） molecules. This study combines homology modeling and molecular dynamics simulation methods to study the structure of CXCRI-IL8 complex. By using CXCR4-vMIP-II crystallography structure as the homologous template, CXCRI-IL8 complex structure was constructed, and then refined using all-atom molecular dynamics simulations. Through extensive simulations, CXCRI-IL8 binding poses were investigated in detail. Furthermore, the role of the N-terminal of CXCR1 receptor was studied by comparing four complex models differing in the N-terminal sequences. The results indicate that the receptor N-terminal affects the binding of IL8 significantly. With a shorter N-terminal domain, the binding of IL8 to CXCR1 becomes unstable. The homology modeling and simulations also reveal the key receptor-ligand residues involved in the electrostatic interactions known to be vital for complex formation.展开更多
This paper explored the feasibility for the application of luminescent CdTe quantum dots prepared in aqueous phase to live cell imaging. The highly luminescent CdTe quantum dots （QDs） were first prepared in aqueous ...This paper explored the feasibility for the application of luminescent CdTe quantum dots prepared in aqueous phase to live cell imaging. The highly luminescent CdTe quantum dots （QDs） were first prepared in aqueous phase, and then were covalentiy coupled to a plant lectin （UEA-1）, as a fluorescent probe. After incubating with of human umbilical vein endothelial cells （HUVECs）, the QD probe with UEA-1 was able to specifically bind the corresponding cell receptor. The good cell images were obtained in live cells using laser confocal scanning microscopy. We predict that QDs prepared in water phase will probably become an attractive alternative probe in cellular imaging and bio-labeling.展开更多
基金the National Natural Science Foundation of China(Nos 20432010, 30570405 and 20672045)
文摘Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl-owth ,survival, and differentiation of specific nerve cell populations. Although animal tests and trials indicate that rhNGF could be ment for diabetic and HIV-related phase- Ⅱ clinical an effective treatneuropathies , a large-scale phase-Ⅲ clinical trial has failed to give similar result. NGF isolated from the mouse submaxillary gland has been widely used clinically in China for the treatment of peripheral neuropathy,
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11575021,U1530401,and U1430237)the National Research Foundation of Korea(Grant Nos.NRF-2017R1A2B2008483 and NRF-2016R1A6A3A04010213)
文摘CXCR1 is a G-protein coupled receptor, transducing signals from chemokines, in particular the interleukin-8 （1L8） molecules. This study combines homology modeling and molecular dynamics simulation methods to study the structure of CXCRI-IL8 complex. By using CXCR4-vMIP-II crystallography structure as the homologous template, CXCRI-IL8 complex structure was constructed, and then refined using all-atom molecular dynamics simulations. Through extensive simulations, CXCRI-IL8 binding poses were investigated in detail. Furthermore, the role of the N-terminal of CXCR1 receptor was studied by comparing four complex models differing in the N-terminal sequences. The results indicate that the receptor N-terminal affects the binding of IL8 significantly. With a shorter N-terminal domain, the binding of IL8 to CXCR1 becomes unstable. The homology modeling and simulations also reveal the key receptor-ligand residues involved in the electrostatic interactions known to be vital for complex formation.
基金This work was financially supported by the National Natural Science Foundation of China （No. 20335020, 90408014） and the Nano-Science Foundation of Shanghai （0452NM052, 05NM0500 -2）.
文摘This paper explored the feasibility for the application of luminescent CdTe quantum dots prepared in aqueous phase to live cell imaging. The highly luminescent CdTe quantum dots （QDs） were first prepared in aqueous phase, and then were covalentiy coupled to a plant lectin （UEA-1）, as a fluorescent probe. After incubating with of human umbilical vein endothelial cells （HUVECs）, the QD probe with UEA-1 was able to specifically bind the corresponding cell receptor. The good cell images were obtained in live cells using laser confocal scanning microscopy. We predict that QDs prepared in water phase will probably become an attractive alternative probe in cellular imaging and bio-labeling.
