[Objective] The study was to screen the active part from roots of Macleaya cordata. [Method] Dipping method was used to study the contact activity of ethanol extract and 4 kinds of extracts by petroleum, ethyl acetate...[Objective] The study was to screen the active part from roots of Macleaya cordata. [Method] Dipping method was used to study the contact activity of ethanol extract and 4 kinds of extracts by petroleum, ethyl acetate, n-butanol and water from the root of M. cordata on 2nd and 6th instars larvae of T. motlitorlin（L.）. [Result] The corrected mortality rate of T. motlitorlin increased with the increase of extract concentration. When the concentration was 100 mg/ml, the contact activity of ethyl acetate extract against 2nd instar larvae of T. motlitorlin was the highest, the corrected mortality rate at 48 h reached 80.95%, and LC50 was 65.532 9 mg/ml. The overall performance of insecticidal activity was as follows： ethyl acetate layer petroleum layer = n-butanol layer total extracts （ethanol） water layer. The contact toxicity of ethanol extract and petroleum extract against 6th instar larvae of T. motlitorlin was the highest, and the corrected mortality rate at 48 h reached 71.42% and 66.67%, LC50 were 83.899 8 and 86.687 7 mg/ml, respectively. The overall performance of insecticidal activity was as follows： total extracts （ethanol） petroleum layer ethyl acetate layer n-butanol layer water layer. [Conclusion] The insecticidal active components of M. cordata root were distributed within the polarity range of petroleum and ethyl acetate, and the part could be further separated and identified.展开更多
基金Supported by Hebei Science and Technology Research and Development Project(10220302D)
文摘[Objective] The study was to screen the active part from roots of Macleaya cordata. [Method] Dipping method was used to study the contact activity of ethanol extract and 4 kinds of extracts by petroleum, ethyl acetate, n-butanol and water from the root of M. cordata on 2nd and 6th instars larvae of T. motlitorlin（L.）. [Result] The corrected mortality rate of T. motlitorlin increased with the increase of extract concentration. When the concentration was 100 mg/ml, the contact activity of ethyl acetate extract against 2nd instar larvae of T. motlitorlin was the highest, the corrected mortality rate at 48 h reached 80.95%, and LC50 was 65.532 9 mg/ml. The overall performance of insecticidal activity was as follows： ethyl acetate layer petroleum layer = n-butanol layer total extracts （ethanol） water layer. The contact toxicity of ethanol extract and petroleum extract against 6th instar larvae of T. motlitorlin was the highest, and the corrected mortality rate at 48 h reached 71.42% and 66.67%, LC50 were 83.899 8 and 86.687 7 mg/ml, respectively. The overall performance of insecticidal activity was as follows： total extracts （ethanol） petroleum layer ethyl acetate layer n-butanol layer water layer. [Conclusion] The insecticidal active components of M. cordata root were distributed within the polarity range of petroleum and ethyl acetate, and the part could be further separated and identified.
