Diversity of bacterial lactase genes in intestinal contents of mice with antibiotics-induced diarrhea

AIM To investigate the diversity of bacterial lactase genes in the intestinal contents of mice with antibiotics-induced diarrhea.METHODS Following 2 d of adaptive feeding, 12 specific pathogenfree Kunming mice were randomly divided into the control group and model group. The mouse model of antibiotics-induced diarrhea was established by gastric perfusion with mixed antibiotics(23.33 m L·kg^(-1)·d^(-1)) composed of gentamicin sulfate and cephradine capsules administered for 5 days, and the control group was treated with an equal amount of sterile water. Contents of the jejunum and ileum were then collected and metagenomic DNA was extracted, after which analysis of bacterial lactase genes using operational taxonomic units(OTUs) was carried outafter amplification and sequencing.RESULTS OTUs were 871 and 963 in the model group and control group, respectively, and 690 of these were identical. There were significant differences in Chao1 and ACE indices between the two groups(P < 0.05). Principal component analysis, principal coordination analysis and nonmetric multidimensional scaling analyses showed that OTUs distribution in the control group was relatively intensive, and differences among individuals were small, while in the model group, they were widely dispersed and more diversified. Bacterial lactase genes from the intestinal contents of the control group were related to Proteobacteria, Actinobacteria, Firmicutes and unclassified bacteria. Of these, Proteobacteria was the most abundant phylum. In contrast, the bacterial population was less diverse and abundant in the model group, as the abundance of Bradyrhizobium sp. BTAi1, Agrobacterium sp. H13-3, Acidovorax sp. KKS102, Azoarcus sp. KH32 C and Aeromonas caviae was lower than that in the control group. In addition, of the known species, the control group and model group had their own unique genera, respectively.CONCLUSION Antibiotics reduce the diversity of bacterial lactase genes in the intestinal contents, decrease the abundance of lactase gene, change the lactase gene strains, and transform their structures.

The Cubic Trigonometric Automatic Interpolation Spline

A class of cubic trigonometric interpolation spline curves with two parameters is presented in this paper. The spline curves can automatically interpolate the given data points and become C^2 interpolation curves without solving equations system even if the interpolation conditions are fixed. Moreover, shape of the interpolation spline curves can be globally adjusted by the two parameters. By selecting proper values of the two parameters,the optimal interpolation spline curves can be obtained.

Planar Cubic G^1 Hermite Interpolant with Minimal Quadratic Oscillation in Average

In this paper we apply a new method to choose suitable free parameters of the planar cubic G1 Hermite interpolant. The method provides the cubic G1 Hermite interpolant with minimal quadratic oscillation in average. We can use the method to construct the optimal shape-preserving interpolant. Some numerical examples are presented to illustrate the effectiveness of the method.

Planar Cardinal Spline Curves with Minimum Strain Energy

In this paper, we focus on how to use strain energy minimization to obtain the optimal value of the fl＇ee parameter of the planar Cardinal spline curves. The unique solution can be easily obtained by minimizing an appropriate approximation of the strain energy. An example is presented to illustrate the effectiveness of our method.

C2 Continuous Quartic Hermite Spline Curves with Shape Parameters

In order to relieve the deficiency of the usual cubic Hermite spline curves,the quartic Hermite spline curves with shape parameters is further studied in this work. The interpolation error and estimator of the quartic Hermite spline curves are given. And the characteristics of the quartic Hermite spline curves are discussed.The quartic Hermite spline curves not only have the same interpolation and continuity properties of the usual cubic Hermite spline curves, but also can achieve local or global shape adjustment and C;continuity by the shape parameters when the interpolation conditions are fixed.

Effects of antibiotics on modern chronic diseases

The discovery of antibiotics is the miracle of modern medicine,which has contributed greatly to the protection of human health.However,in despite of medical advances to date,some quite inexplicable modern diseases are still haunting us.These chronic diseases are less fierce in fatality,but more permanently compromise the quality of patients′lives.In this paper,the relevant background knowledge about antibiotics were introduced and the relationship between antibiotics and several common chronic diseases was analyzed,in order to promote the rational use of antibiotics and the correct view on antibiotics.