Survey and Analysis on Alien Invasive Plants of Jinziling Scenic Spot in Pingle County

According to the field survey of alien invasive plants of Jinziling,there were 23 kinds of alien invasive plants in the scenic spot.Species with the comprehensive dominance degree higher than 10% were as follows: six kinds of Asteraceae: Bidens pilosa,Aster subulatus,Ageratum conyzoides,Conyzai canadens,Parthenium hysterophorus,and Conyza sumatrensis; two kinds of Gramineae: Axonopus compressu,Eleusine indica; two kinds of convolvere: Ipomoea nil,Ipomoea purpurea; two kinds of Fabaceae: Cassiae tora,Medicago hispida; one kind of Verbenaceae: Lantana camara; one kind of Euphorbiaceae: Euphorbia hirta.In total,there were 14 kinds of alien invasive plants.In this survey,the dominant species of invasive plants were obtained by statistical analysis of the composition and distribution of the invasive plants,and the invasion trend was predicted,so as to take effective and economical management and control measures.

Genome-wide identification,expression and functional analysis of sugar transporters in sorghum(Sorghum bicolor L.)

Sugar transporters are essential for osmotic process regulation,various signaling pathways and plant growth and development.Currently,few studies are available on the function of sugar transporters in sorghum(Sorghum bicolor L.).In this study,we performed a genome-wide survey of sugar transporters in sorghum.In total,98 sorghum sugar transporters(SSTs)were identified via BLASTP.These SSTs were classified into three families based on the phylogenetic and conserved domain analysis,including six sucrose transporters(SUTs),23 sugars will eventually be exported transporters(SWEETs),and 69 monosaccharide transporters(MSTs).The sorghum MSTs were further divided into seven subfamilies,including 24 STPs,23 PLTs,two VGTs,four INTs,three p Glc T/SBG1 s,five TMTs,and eight ERDs.Chromosomal localization of the SST genes showed that they were randomly distributed on 10 chromosomes,and substantial clustering was evident on the specific chromosomes.Twenty-seven SST genes from the families of SWEET,ERD,STP,and PLT were found to cluster in eight tandem repeat event regions.In total,22 SSTs comprising 11 paralogous pairs and accounting for 22.4%of all the genes were located on the duplicated blocks.The different subfamilies of SST proteins possessed the same conserved domain,but there were some differences in features of the motif and transmembrane helices(TMH).The publicly-accessible RNA-sequencing data and real-time PCR revealed that the SST genes exhibited distinctive tissue specific patterns.Functional studies showed that seven SSTs were mainly located on the cell membrane and membrane organelles,and 14 of the SSTs could transport different types of monosaccharides in yeast.These findings will help us to further elucidate their roles in the sorghum sugar transport and sugar signaling pathways.

Investigation and Analysis of Alien Plants in the Scenic Spot Chengbi Lake

A comprehensive survey and analysis of alien invasive plants at the scenic spot Chengbi Lake was conducted by combining route investigation with interviews and quadrat sampling.There were 21 families,33 genera and 42 species in the scenic spot Chengbi Lake.Among them,herbal plants accounted for 69%.The Compositae and Amaranthaceae families were the most frequent,represented by 18 species in total;and 36 species of these alien invasive plants originated from America,accounting for 85.7% of the total.Fifty-seven percent of the species were introduced as ornamental plants.Seven of them were severely harmful,including Wedelia trilobata,Eupatorium odoratum,Lantana camara,Mirabilis jalapa,Solanum erianthum,Ipomoea cairica and Alternanthera philoxeroides.Strategies to increase defensive awareness,reinforce ecological protection,regulate species introduction and improve supervision mechanisms are discussed.

Occurrence and Distribution of Alternanthera philoxeroides in the Youjiang River Valley

The occurrence and distribution of Alternanthera philoxeroides in different habitats of four districts and counties in the Youjiang River Valley were investigated from September to November in 2018. The results show that A. philoxeroides certainly has occurred in watercourses, ridges, ditches, roadsides and green belts in those four districts and counties of The Youjiang valley. According to the sampling investigation, ridges have the highest frequency of occurrence, followed by ditches and roadsides. The A. philoxeroides living in ditches grows better than other four habitats, and the plants are stronger and vigorous. In the geographical division, the distribution areas of A. philoxeroides in Tianyang District and Tiandong County are the largest. Youjiang District and Pingguo County have a small amount of A. philoxeroides distributed along farmland, green belts and village houses, which has the characteristics of high local population density and strong aggregation, but the areas are smaller than other counties.

Acetylcholinesterase Biosensor Based on Poly(diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

In this paper, an amperometric acetylcholinesterase(ACh E) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly(diallyldimethy-lammonium chloride)-multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode(GCE) surface, then ACh E was immobilized onto the modified GCE to fabricate the ACh E biosensor. The morphologies and electrochemistry properties of the prepared ACh E biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the ACh E activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis.