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豇豆退绿斑驳病毒(CCMV)突变体的混合侵染研究
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作者 杨普云 RichardF.ALLISON 《植物病理学报》 CAS CSCD 北大核心 2002年第1期59-64,共6页
本研究采用 PCR点突变的方法 ,对豇豆退绿斑驳病毒 (CCMV) RNA3的 c DNA克隆进行突变 ,突变体 TP7是在外壳蛋白基因之前突变形成一个独特的 Sal I酶切点 ;TP8是在 3a基因之前突变形成一个独特的 Bam H I酶切点 ;AG 1是在外壳蛋白基因之... 本研究采用 PCR点突变的方法 ,对豇豆退绿斑驳病毒 (CCMV) RNA3的 c DNA克隆进行突变 ,突变体 TP7是在外壳蛋白基因之前突变形成一个独特的 Sal I酶切点 ;TP8是在 3a基因之前突变形成一个独特的 Bam H I酶切点 ;AG 1是在外壳蛋白基因之后突变形成一个独特的 Not I酶切点。TP7、TP8、AG1体外转录产物分别与 CCMV RNA1、RNA2的 c DNA克隆的体外转录产物混和后接种豇豆 ,结果表明 ,接种后第 6 d TP7植株首先表现出症状 ,9d后 TP8、AG1和野生型 CCMV接种的植株表现出症状 ,TP7的症状发展最快。采用 TP7、TP8、AG1接种 2 0 d的毒源植株 ,按 TP7+ TP8、TP7+ AG1、TP8+ AG1等量混合汁液接种豇豆 ,接种 2 0 d,通过 RT- PCR分析接种植物 ,在 TP7+ TP8和 TP7+ AG1混合侵染中 ,TP7在混合侵染中占优势 ,在 TP8+ AG1的混合侵染中 ,TP8和 AG1的种群十分接近。 展开更多
关键词 豇豆 退绿斑驳病毒 混合侵染 突变体 RNA病毒
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平菇中3种球形病毒的鉴定及其特性研究(英文) 被引量:9
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作者 YuHyunJae LeeJungSun +15 位作者 LeeNamJoo HaSiJung KimSoYeon GangEunJoo BaeDongWon ChangMooUng LeeHyunSook YuHyunJae LeeJungSun LeeNamJoo HaSiJung KimSoYeon GangEunJoo BaeDongWon ChangMooUng LeeHyunSook 《华中农业大学学报》 CAS CSCD 北大核心 2004年第1期150-156,共7页
在韩国的平菇(Pleurotus ostreatus)商品化种植基地发现了真菌病毒的自然流行为害的现象。这些病害经常伴随着一些球形病毒粒体的出现。采用Tris-EDTA缓冲液提取,PEG—NaCl沉淀和CsCl梯度离心,依据不同的比重,获得了3种病毒粒体,其中1... 在韩国的平菇(Pleurotus ostreatus)商品化种植基地发现了真菌病毒的自然流行为害的现象。这些病害经常伴随着一些球形病毒粒体的出现。采用Tris-EDTA缓冲液提取,PEG—NaCl沉淀和CsCl梯度离心,依据不同的比重,获得了3种病毒粒体,其中1种病毒粒体直径为27 nm,另外2种的粒体为27nm,并推定这些病毒共同感染栽培的平菇。其中1种27 nm的球形病毒,其比重较34 nm的病毒大,称为平菇球形病毒(OMSV),该病毒含有2条大小分别为6.00 kb和1.25 kb的ssRNA片段。直径为34 nm的病毒有两种比重小的粒体,其中1种常与OMSV共感染平菇,称为平菇等面体病毒-I(oyster mush—room isometric virus—I,OMIV-1),该病毒含有12条dsRNA片段,它们的大小分别是2.65,2.45,2.40,2.20,2.15,2.10,2.05,2.00,1.90,1.80,1.00和0.80 kb。另外一种称之为平菇等面体病毒-Ⅱ(oyster mushroom isometric virus-Ⅱ,OMIV-Ⅱ),含有3条dsRNA片段,大小分别是2.25,2.15和2.05kb。经聚丙烯酰胺凝胶(12%)电泳、考马斯量兰染色表明OMSV,OMIV-Ⅰ和OMIV-Ⅱ病毒粒体的外壳蛋白的分子量大小分别是29 kDa、71 kDa和62 kDa。这些结果表明在平菇中至少存在3种不同的ssR-NA和dsRNA病毒。 展开更多
关键词 平菇 球形病毒 鉴定 外壳蛋白 分子量 鉴定
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The dielectronic recombination process in laser-produced Au plasmas 被引量:1
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作者 焦荣珍 程新路 杨向东 《Chinese Physics B》 SCIE EI CAS CSCD 2003年第10期1140-1142,共3页
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Resonance structure for 4s subshell in photoionization of Mn
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作者 LUPeng-fei YANGXiang-dong +2 位作者 BAIYu-lin LIUJin-chao LIUGang 《原子与分子物理学报》 CAS CSCD 北大核心 2004年第2期195-198,共4页
It is shown that the 3d5(4X) 4s(5X)of 4s satellites ,except to the coupling between 3d54s(7,5S) and 3p→ 3d transition ,plays a key role on the magnitude of photoionization of 4s cross section .The coupled equation me... It is shown that the 3d5(4X) 4s(5X)of 4s satellites ,except to the coupling between 3d54s(7,5S) and 3p→ 3d transition ,plays a key role on the magnitude of photoionization of 4s cross section .The coupled equation methodis improvedto calculate this resonance by including these channels .The results of calculations are compared with the experimental data from 4 6eV to 5 6eV photon energies,which are in good agreement with the experiment. 展开更多
关键词 MANY-BODY PERTURBATION theory Coupled EQUATION method RESONANCE structure
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韩国Dermaptera寄生真菌虫囊菌目的种类记录(英文)
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作者 LeeYongBo YangYoungKi +3 位作者 LimChaeKyu LeeYongBo YangYoungKi LimChaeKyu 《华中农业大学学报》 CAS CSCD 北大核心 2004年第1期14-21,共8页
从韩国Dermaptera分离获得了虫囊菌目的2个属的2种真菌。其中Dimeromyces anisolabisThaxter寄生Anisolabis maritima(Bonelli)是韩国的新记录种,Filariomyces for ficulae Shanor寄生Labidura riparia japonica(de Haan)于济州岛上首... 从韩国Dermaptera分离获得了虫囊菌目的2个属的2种真菌。其中Dimeromyces anisolabisThaxter寄生Anisolabis maritima(Bonelli)是韩国的新记录种,Filariomyces for ficulae Shanor寄生Labidura riparia japonica(de Haan)于济州岛上首次发现。这些真菌标本和菌株保存在韩国Chosun大学的教育学院科学教育系的微生物实验室。 展开更多
关键词 韩国 虫囊菌目 Dermaptera 寄生真菌 新记录种
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Fe-Ti oxide and Fe-Cu sulfide exsolution in amphibole cumulate xenoliths from granodiorite in Tongling,Anhui Province 被引量:11
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作者 QINXinlong DUYangsong +2 位作者 H.K.Lee YINJingwu GUOXifeng 《Chinese Science Bulletin》 SCIE EI CAS 2003年第23期2626-2634,共9页
A number of Fe-Ti oxide and Fe-Cu sulfide exsolution phases are found in amphibole cumulate xenoliths from Mesozoic granodioritic plutons at Jiguanshi and Jin- kouling in Tongling, Anhui Province. Most of amphiboles i... A number of Fe-Ti oxide and Fe-Cu sulfide exsolution phases are found in amphibole cumulate xenoliths from Mesozoic granodioritic plutons at Jiguanshi and Jin- kouling in Tongling, Anhui Province. Most of amphiboles in the xenoliths have been identified as pargasite, and deter-mined as a crystallization phase in the lower crust. The oxide exsolutions commonly acicular, are distributed parallel to [100] of amphibole with a typical Schiller texture. They con-tain 0.29%—51.07% of TiO2, and correspond to magnetite, titaniferous magnetite, titanomagnetite or ilmenite. All ex-solved sulfide grains, being rounded to extended rounded in shape, are regularly arranged in sub-paralleled or closely beaded groups, except for a few of grains that are scattered or isolated. The exsolved sulfide phases comprise mostly pyrrhotite, secondarily cubanite and chalcopyrite, and rarely pyrite. A few grains of magnetite can coexist with pyrrhotite in most samples. The sulfide phases are poor in nickel (usu-ally Ni < 0.5%, Ni/Fe < 0.003), while relative enrichments in copper (up to 2.93%) typify the pyrrhotite. Additionally, both oxides and sulfides may be exsolved in the same area of amphibole. Occurrence of Fe-Ti oxide and Fe-Cu sulfide exsolution phases in the xenoliths leads to inference that ore-forming materials such as S, Fe, Cu, etc. have been pri-marily dissolved in Mesozoic magma within the lower crust beneath the Tongling area. 展开更多
关键词 闪石 捕虏岩 花岗闪长岩 安徽 Fe-Ti氧化物 Fe-Cu硫化物
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Passive Control of Transonic Flow Fields with Shock Wave Using Non-equilibrium Condensation and Porous Wall 被引量:1
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作者 MasanoriTanaka Shigerumatsuo +3 位作者 ToshiakiSetoguchi Kenjikaneko Heuy-DongKim ShenYu 《Journal of Thermal Science》 SCIE EI CAS CSCD 2003年第2期126-131,共6页
When non-equilibrium condensation occurs in a supersonic flow field.the flow is affected by the latent heat released.In the present study.in order to control the transonic flow field with shock wave,a condensing flow ... When non-equilibrium condensation occurs in a supersonic flow field.the flow is affected by the latent heat released.In the present study.in order to control the transonic flow field with shock wave,a condensing flow was produced by an expansion of moist air on a circular bump model and shock waves were occurred in the supersonic parts of the fields.Furthermore,the additional passive technique of shock /boundary layer interaction using the porous wall with a cavity underneath was adopted in this flow .The effects of these methods on the shock wave characteristics were investigated numerically and experimentally.The result obtained showed that the total pressure loss in the flow fields might be effectively reduced by the suitable combination between non-equilibrium condensation and the position of porous wall. 展开更多
关键词 跨音速流动 非平衡压缩 边界层 流动控制 多孔渗水 冲击波
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