Jetting-based bioprinting facilitates contactless drop-on-demand deposition of subnanoliter droplets at well-defined positions to control the spatial arrangement of cells,growth factors,drugs,and biomaterials in a hig...Jetting-based bioprinting facilitates contactless drop-on-demand deposition of subnanoliter droplets at well-defined positions to control the spatial arrangement of cells,growth factors,drugs,and biomaterials in a highly automated layer-by-layer fabrication approach.Due to its immense versatility,jetting-based bioprinting has been used for various applications,including tissue engineering and regenerative medicine,wound healing,and drug development.A lack of in-depth understanding exists in the processes that occur during jetting-based bioprinting.This review paper will comprehensively discuss the physical considerations for bioinks and printing conditions used in jetting-based bioprinting.We first present an overview of different jetting-based bioprinting techniques such as inkjet bioprinting,laser-induced forward transfer bioprinting,electrohydrodynamic jet bioprinting,acoustic bioprinting and microvalve bioprinting.Next,we provide an in-depth discussion of various considerations for bioink formulation relating to cell deposition,print chamber design,droplet formation and droplet impact.Finally,we highlight recent accomplishments in jetting-based bioprinting.We present the advantages and challenges of each method,discuss considerations relating to cell viability and protein stability,and conclude by providing insights into future directions of jetting-based bioprinting.展开更多
Three-dimensional(3D)printing is a highly automated platform that facilitates material deposition in a layer-by-layer approach to fabricate pre-defined 3D complex structures on demand.It is a highly promising techniqu...Three-dimensional(3D)printing is a highly automated platform that facilitates material deposition in a layer-by-layer approach to fabricate pre-defined 3D complex structures on demand.It is a highly promising technique for the fabrication of personalized medical devices or even patient-specific tissue constructs.Each type of 3D printing technique has its unique advantages and limitations,and the selection of a suitable 3D printing technique is highly dependent on its intended application.In this review paper,we present and highlight some of the critical processes(printing parameters,build orientation,build location,and support structures),material(batch-to-batch consistency,recycling,protein adsorption,biocompatibility,and degradation properties),and regulatory considerations(sterility and mechanical properties)for 3D printing of personalized medical devices.The goal of this review paper is to provide the readers with a good understanding of the various key considerations(process,material,and regulatory)in 3D printing,which are critical for the fabrication of improved patient-specific 3D printed medical devices and tissue constructs.展开更多
The article Jetting-based bioprinting:process,dispense physics,and applications,written by Wei Long Ng and Viktor Shkolnikov,was originally published electronically on the publisher’s internet portal on 12 July 2024 ...The article Jetting-based bioprinting:process,dispense physics,and applications,written by Wei Long Ng and Viktor Shkolnikov,was originally published electronically on the publisher’s internet portal on 12 July 2024 without open access.展开更多
针对混合现实技术在识别标志物时易发生抖动,且识别过程易受到遮挡影响的问题,从标志物特征点提取与匹配的角度入手,改进混合现实技术对标志物的识别算法。通过构造尺度空间,结合加速稳健特征(speeded up robust features,SURF)算法提...针对混合现实技术在识别标志物时易发生抖动,且识别过程易受到遮挡影响的问题,从标志物特征点提取与匹配的角度入手,改进混合现实技术对标志物的识别算法。通过构造尺度空间,结合加速稳健特征(speeded up robust features,SURF)算法提取特征点,对ORB(oriented FAST and rotated BRIEF)算法的特征点提取和匹配进行改进。改进后的算法在特征点匹配的过程中精度更高,比SURF算法提升了38.8%,比ORB算法提升了28.3%,有效地提高了目标识别的效率。结果表明:把改进后的算法运用在混合现实系统中,可以在标志物被遮挡50%时,成功把虚拟模型叠加在标志物上,解决了模型抖动的问题。展开更多
基金support from NTU Presidential Postdoctoral Fellowship.
文摘Jetting-based bioprinting facilitates contactless drop-on-demand deposition of subnanoliter droplets at well-defined positions to control the spatial arrangement of cells,growth factors,drugs,and biomaterials in a highly automated layer-by-layer fabrication approach.Due to its immense versatility,jetting-based bioprinting has been used for various applications,including tissue engineering and regenerative medicine,wound healing,and drug development.A lack of in-depth understanding exists in the processes that occur during jetting-based bioprinting.This review paper will comprehensively discuss the physical considerations for bioinks and printing conditions used in jetting-based bioprinting.We first present an overview of different jetting-based bioprinting techniques such as inkjet bioprinting,laser-induced forward transfer bioprinting,electrohydrodynamic jet bioprinting,acoustic bioprinting and microvalve bioprinting.Next,we provide an in-depth discussion of various considerations for bioink formulation relating to cell deposition,print chamber design,droplet formation and droplet impact.Finally,we highlight recent accomplishments in jetting-based bioprinting.We present the advantages and challenges of each method,discuss considerations relating to cell viability and protein stability,and conclude by providing insights into future directions of jetting-based bioprinting.
文摘Three-dimensional(3D)printing is a highly automated platform that facilitates material deposition in a layer-by-layer approach to fabricate pre-defined 3D complex structures on demand.It is a highly promising technique for the fabrication of personalized medical devices or even patient-specific tissue constructs.Each type of 3D printing technique has its unique advantages and limitations,and the selection of a suitable 3D printing technique is highly dependent on its intended application.In this review paper,we present and highlight some of the critical processes(printing parameters,build orientation,build location,and support structures),material(batch-to-batch consistency,recycling,protein adsorption,biocompatibility,and degradation properties),and regulatory considerations(sterility and mechanical properties)for 3D printing of personalized medical devices.The goal of this review paper is to provide the readers with a good understanding of the various key considerations(process,material,and regulatory)in 3D printing,which are critical for the fabrication of improved patient-specific 3D printed medical devices and tissue constructs.
文摘The article Jetting-based bioprinting:process,dispense physics,and applications,written by Wei Long Ng and Viktor Shkolnikov,was originally published electronically on the publisher’s internet portal on 12 July 2024 without open access.
文摘针对混合现实技术在识别标志物时易发生抖动,且识别过程易受到遮挡影响的问题,从标志物特征点提取与匹配的角度入手,改进混合现实技术对标志物的识别算法。通过构造尺度空间,结合加速稳健特征(speeded up robust features,SURF)算法提取特征点,对ORB(oriented FAST and rotated BRIEF)算法的特征点提取和匹配进行改进。改进后的算法在特征点匹配的过程中精度更高,比SURF算法提升了38.8%,比ORB算法提升了28.3%,有效地提高了目标识别的效率。结果表明:把改进后的算法运用在混合现实系统中,可以在标志物被遮挡50%时,成功把虚拟模型叠加在标志物上,解决了模型抖动的问题。
