Objective:To establish a rapid and simple assay for the diagnosis of Chlamydia trachomatis (CT) infection. Methods BALB/c mice were immunized with SDS-PAGE purified major outer membrane protein (MOMP) from CT and the ...Objective:To establish a rapid and simple assay for the diagnosis of Chlamydia trachomatis (CT) infection. Methods BALB/c mice were immunized with SDS-PAGE purified major outer membrane protein (MOMP) from CT and the monoclonal antibodies were obtained subsequently. Two-site ELISA was developed to detect CT infection. Results: The established assay was able to detect as low as 1.248ug/ml MOMP with interrun and inrun CV 6.9% and 3.1% respectively. 94% (34/36) of culture-positive samples were found to be positive in the current examination, indicating the high sensitivity of this assay. Conclusion: The assay is applicable for clinical diagnosis of CT infection.展开更多
Toxoplasma gondii （T. gondfi） is one of the intracellular parasitized protozoa and may cause severe medical complications in fetus or immunocompromised individuals. T. gondii existed as tachyzoite during acute stag...Toxoplasma gondii （T. gondfi） is one of the intracellular parasitized protozoa and may cause severe medical complications in fetus or immunocompromised individuals. T. gondii existed as tachyzoite during acute stage while as bradyzoite during chronic phase in human cells. To improve understanding of the prevention, diagnosis, and treatment of the disease, it is important to explore the distribution and fluctuation and other biological features of T. gondii in host. The trophozoite had been found in the saliva, blood or urine of the host. Some studies suggested the dynamic changes of circulating antibody and toxoplasma circulating antigen （TCA） either in blood or in urine. T. gondii in tissue or blood cannot be counted exactly under the microscope because it was only several micrometers in size and thus most of the studies were performed qualitatively by mouse inoculation or immunology methods. The quantitative fluorescent polymerase chain reaction （QF-PCR） and its application raised the possibility for dynamic observation of the polypide in the host. In this study, blood and semen were collected from the male rabbit model infected with toxoplasma tachyzoites and T. gondii was detected by QF-PCR quantitatively.展开更多
文摘Objective:To establish a rapid and simple assay for the diagnosis of Chlamydia trachomatis (CT) infection. Methods BALB/c mice were immunized with SDS-PAGE purified major outer membrane protein (MOMP) from CT and the monoclonal antibodies were obtained subsequently. Two-site ELISA was developed to detect CT infection. Results: The established assay was able to detect as low as 1.248ug/ml MOMP with interrun and inrun CV 6.9% and 3.1% respectively. 94% (34/36) of culture-positive samples were found to be positive in the current examination, indicating the high sensitivity of this assay. Conclusion: The assay is applicable for clinical diagnosis of CT infection.
基金This work was supported by grants from Henan Innovation Project for University Prominent Research Talents (No. 2006KYCX011) and the key project of Science and Technology Department of Henan (No. 0524410051).
文摘Toxoplasma gondii （T. gondfi） is one of the intracellular parasitized protozoa and may cause severe medical complications in fetus or immunocompromised individuals. T. gondii existed as tachyzoite during acute stage while as bradyzoite during chronic phase in human cells. To improve understanding of the prevention, diagnosis, and treatment of the disease, it is important to explore the distribution and fluctuation and other biological features of T. gondii in host. The trophozoite had been found in the saliva, blood or urine of the host. Some studies suggested the dynamic changes of circulating antibody and toxoplasma circulating antigen （TCA） either in blood or in urine. T. gondii in tissue or blood cannot be counted exactly under the microscope because it was only several micrometers in size and thus most of the studies were performed qualitatively by mouse inoculation or immunology methods. The quantitative fluorescent polymerase chain reaction （QF-PCR） and its application raised the possibility for dynamic observation of the polypide in the host. In this study, blood and semen were collected from the male rabbit model infected with toxoplasma tachyzoites and T. gondii was detected by QF-PCR quantitatively.
