Effects of p16 gene on biological behavious in hepatocellular carcimoma cells

AIM:To investigate the effects of p16 gene on biological behavious in hepatocellular carcinoma cells.

Dissociation, culture and morphologic changes of interstitial cells of Cajal in vitro

AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg2+ and collagenase, and stem cell factor, etc., were investigated.ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro.RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d.CONCLUSION: Many factors can influence the dissociation and culture of ICC.

Effects of mu and kappa opioid receptor agonists and antagonists on contraction of isolated colon strips of rats with cathartic colon

AIM: To study the effects of mu and kappa opioid receptor agonists and antagonists on the isolated colon strips of rats with cathartic colon. METHODS: Cathartic colon model was established by feeding rats with contact laxatives, and effects of mu and kappa opioid receptor agonists and antagonists on electricity-stimulated contraction of isolated colon strips of rats with cathartic colon were observed. RESULTS: Compared with control group, exogenous mu and kappa agonists inhibited significantly electricity-stimulated contraction of strips of cathartic colon (8.50±0.89 mm, 6.24±0.91 mm, 3.35±0.6 mm vs 11.40±0.21 mm P<0.01; 8.98±0.69 mm, 6.89±0.71 mm, 4.43±0.99 mm vs 11.40±0.21 mm, P<0.01). In contrast, the exogenous mu antagonist significantly enhanced electricity-stimulated contraction of isolated colon strips (13.18±0.93 mm, 15.87±0.98 mm, 19.46±1.79 mm vs 11.40±0.21 mm, P<0.01), but kappa antagonist had no effect on the isolated colon strips of rats with cathartic colon. CONCLUSION: Muand kappa opioid receptors are involved in the regulation of colon motility of rats with cathartic colon.

H pylori are associated with chronic cholecystitis

AIM： To study whether H pylon are associated with chronic cholecystitis. METHODS： The subjects were divided into three groups： H pylori-infected cholecystitis group, H pylorinegative cholecystitis group and control group. Pathologic changes of the gallbladder were observed by optic and electronic microscopes and the levels of interleukin-1, 6 and 8 （IL-1, 6 and 8） were detected by radioimmunoassay. RESULTS： Histological evidence of chronic cholecystitis including degeneration, necrosis, inflammatory cell infiltration, were found in the region where H pylori colonized. Levels of IL-1, 6 and 8 in gallbladder mucosa homogenates were significantly higher in H py/oriinfected cholecystitis group than those in Hpylorinegative cholecystitis group and control group. CONCLUSION： H pylon infection may be related to cholecystitis.

Correlative Study on MRI Morphologic Features,Pathology, and Molecular Biology of Breast Cancer

OBJECTIVE To investigate the correlation among MRI morphologic features,pathology, and molecular biology of breast cancer.METHORIBS MRI was used to analyze the morphologic features of breast cancers of 78 patients before operation. The mastectomy specimens of the breast neoplasms were immunohistochemically stained, and the expression of the estrogen (ER), progesterone receptor (PR), C-erbB-2, P53, and the distribution of microvessel density (MVD) measured. The pathologic results were compared with the MRI features.RESULTS Among the 80 breast cancers, ER positive expression was positively correlated with the spicular contour of breast cancers (P<0.01),while showing a significant inverse correlation with the T-stage (P<0.05). CerbB-2 and P53 positive expression were positively correlated With the necrotic center of the cancers (P<0.05). The expression of PR was not significantly correlated with the spicular contour, obscure margin, necrotic center, and T-stage of these cancers (P>0.05). Among 41 breast cancers examined with dynamic contrast enhanced MR, there was a positive correlation between the spatial distribution of the contrast agent and MVD (P<0.01).CONCLUSION To a certain extent there is some correlation among the MRI morphologic features, pathology, and molecular biological factors in breast cancer. The biological behavior and prognosis of breast cancer can be assessed based on MRI features.

Immune-related DNA methylation signature associated with APLN expression predicts prognostic of hepatocellular carcinoma

TIME,Immunity,Prognosis,BioinformaticsThis study used transcriptome and epigenetic data to predict the prognosis of immune-related genes(IRGs)Apelin(APLN)in patients with hepatocellular carcinoma(HCC).The TCGA database has gene expression and clinical data for HCC.And DNA methylation 450 k data for HCC was download from the University of California Santa Cruz(UCSC)Xena browser.Performing clinical and prognostic analysis of APLN expression,results show that APLN is highly expressed in tumor samples.And it has an increasing trend with the development of clinical stage and T stage.To explore the prognostic role of APLN,the Immune-related DNA methylation(DNAm)sites associated with APLN analyzed by bioinformatics.Univariate COX screened the methylation sites that are related to both APLN and survival.The risk score related to methylation site signature was determined according to their least absolute shrinkage and selection operator(LASSO)coefficients.Then the patients were divided into high-risk groups and low-risk groups.Significant differences in overall survival(OS)were found in the training cohort.Nomogram shows that APLN or methylation signature can effectively predict the prognosis of HCC patients.In summary,APLN may be a diagnostic and prognostic marker for HCC.

The role of angiotensinⅡtype 1 receptor pathway in cerebral ischemia-reperfusion injury:Implications for the neuroprotective effectof ARBs

Cerebral ischemia-reperfusion(I/R)injury is a crucial factor that impacts the prognosis of recanalization therapy for acute ischemic stroke(AIS).It has been found that the brain renin-angiotensin system,especially the angiotensinⅡtype 1 receptor(AT1R)pathway,plays a significant role in cerebral I/R injury.This pathway is involved in processes such as oxidative stress,neuroinflammation,apoptosis,and it affects cerebrovascular autoregulation and the maintenance of blood-brain barrier.AT1R blocker(ARB),widely used as an antihypertensive agent,has demonstrated stroke prevention capabilities in numerous prospective studies,independent of its antihypertensive characteristics.Studies focusing on neurological diseases like Alzheimer's disease,Parkinson's disease,and cognitive impairment have confirmed that ARBs exhibit neuroprotective effects and aid in improving neurological functions.Preclinical studies have shown that ARBs can reduce infarct volume and brain edema,inhibit multiple signaling pathways associated with I/R injury,restore energy levels in damaged brain regions,and rescue the penumbra by promoting neovascularization in cerebral I/R models.These findings suggest that ARBs have potential to become a novel category of neuroprotecting agents for clinical treatment of Als.Therefore,this review primarily provides a theoretical foundation and practical evidence for the future clinical utilization of ARBs as neuroprotective agents following reperfusion therapy for Als.It outlines the role of cerebral I/R injury through the AT1R pathway and highlights the research progressmadeonARBs in I/Rmodels.

Animal study on lung injury caused by simulant segmented shock waves

To study the lung injury caused by segmented shock waves. Methods: A total of 60 rabbits and 20 rats were used in this study. The process of transmission of shock waves was divided into three phases, i.e., the recompression phase (RP), the decompression phase (DP) and the underpressure phase (UP). And the recompression wave (RW), the decompression wave (DW) and the underpressure wave (UW) simulated the three phases, respectively, generated by the equipment designed by us. The RW test, DW test and UW test were respectively applied to the animals. And lung injuries caused by segmented shock waves were discussed. Results: Under the experimental conditions, the RW did not cause obvious lung injury, but the DW could cause different severities of lung injuries. The greater the decompression ranged and the shorter the decompression duration was adopted, the more severe the lung injury was observed. The UW, to some extent, could cause obvious lung injury. Conclusions: It suggests that lung injury under shock waves probably occurs during the DP primarily. It probably does not cause direct obvious lung injury during the RP, but significantly influences the capability of causing lung injury during the DP.

Rabbit lung injury induced by explosive decompression

Objective: To study the mechanism of rabbit lung injury caused by explosive decompression. Methods: A total of 42 rabbits and 10 rats were served as the experimental animals. A slow recompression decompression test and an explosive decompression test were applied to the animals, respectively. And the effects of the given tests on the animals were discussed. Results: The slow recompression decompression did not cause an obvious lung injury, but the explosive decompression did cause lung injuries in different degrees. The greater the decompression range was, the shorter the decompression duration was, and the heavier the lung injuries were. Conclusions: Explosive decompression can cause a similar lung injury as shock wave does. The primary mechanical causes of the lung injury might be a tensile strain or stress in the alveolar wall and the pulmonary surfaces impacts on the inside wall of the chest.

Application of injury severity score to multiple injuries headed by spinal cord injury

Objective： To evaluate the application of injury severity score （ISS） to multiple injuries headed by spinal cord injury. Methods： The data of 55 cases （40 males and 15 females, aged 17-69 years, mean = 41 years） of multiple injuries headed by spinal cord injury treated in our hospital from January 2000 to December 2004 were reviewed and analyzed with ISS （Version of AIS-2005 ） to explore their relationship. Results ： The ISS values increased with the number of injured regions, so did the complications. The recovery rate was negatively related to ISS values. During the period of immunity observation, the patients with ISS 〉 25 could undergo planned operations safely. Conclusions： Treatment for multiple injuries headed by spinal cord injury closely depends on the general and local conditions of the patients. ISS may provide useful data for the choice of treatment methods.

Endogenous glucocorticoid increases the basal level of Treg-Th17 balance under early phase of stress

Objective： To explore the changes of Treg-Thl 7 balance influenced by corticosterone, major ef- fect hormone ofhypothalamic-pituitary-adrenal （HPA） axis under running stress. Methods： Atotal of 25 corticotropin-releasing hor- mone （CRH） wildtype （CRH＋/＋） and knockout （CRH-/-） mice were adopt and divided into 4 groups as follows： CRH＋/＋ ctrl, CRH＋/＋ stress, CRH-/- ctrl and CRH-/- stress. All mice in stress groups were under 2 h running. After 1 h, blood plasma in all groups was collected and the ex- pression of corticosterone and IL- 17A was detected by ELISA. Meanwhile, unicell suspensions of peripheral lymph node and spleen in each group were prepared too and stained by PE-CD4 and FITC-CD25, then the changes of Treg （CD4＋CD25＋） in different groups were checked by flow cytometry; all data were statistically analyzed by the software of WinMDI 2.9, SPSS 11.5, Origin 7.5 and Matlab 2-D and 3-D plot function. Results： The levels of corticosterone were signifi- cantly higher in stress groups than that in correspond- ing control groups （P〈0.05）, especially in CRH＋/＋ stress group （P〈0.01）. However, the changes of Tregs were not obvious between stress groups and control groups with respective genotypes （P〈0.05）. Compared with that in CRH＋/＋ control group, the ratio of Treg and the expres- sion of IL-17A in CRH-/- stress group were significantly higher than those in control group （P〈0.05）. Combined with the expression levels of corticosterone, Treg and Thl7, our study suggests that endogenous glucocorti- cold with basal level may cause the changes in Treg- Thl7 balance. Moreover, as the corticosterone level increases, the expression of Treg and Thl7 appears to manifest antagonistic fluctuant status with a rising ten- dency in general. Conclusion： Endogenous glucocorticoid under early stage of stress may increase the function of T lymphocyte immunity to some extent.

Research advances of traffic medicine in China

Objective:To briefly introduce the increase in the number of motor vehicles in our country and main research advances of traffic medicine.Methods:To collect the relative data issued by government and the papers pulished in the newspapers or medical journals,and analyze them with combination of our own research work. Results:1.The number of motor vehicles in our country in 1997 was 680.02 times more than that in 1951,and increases t 42.0932 million from 1.5887 million over the past two decades(1978-1997)since the reform and opening,with net increase of 40.5045 million,increasing about 25 times.During the same period,the number of motorcycles increased to 20.2221 million from 104.3 thousand,increasing about 193 times.There were 4.4 hundred million bicycles,accounting for one third or the total world wide,Over 30% to 50% of eople in cities used bicycles as their transport tool when they go out.2.In the 1990's (9190 to 1997),the number of motor vehicles increased 1.85 times,while road traffic accidents(RATs) only increased 0.22 times,the deaths and the injuries 0.5 and 0.23 times only,respectively,indicating that the increasing tendency of RTAs and casualties was controlled to some extent.3.The prople of 21 to 45 years old were dominant among all casualties.The sequence of deaths in order was pedestrians(26.5%),vehicle drivers(24.8%),passengers(24.3%),bicyclists(18.4%)and others (6.1%).4.Concerning the accident causes,human faults accounted for 92.9%,83.6% of RTAs wre due to the faults of motor vehicle drivers,and 1.5% of RTAs were due to driving after drinking with deaths occupying 2.9% of the total.5.Proneness to RTSs:6%-8% of motor vehical drivers had proneness to RTA,causing 35%-40% fo the totoal RTAs.6.Various bio-impact machines were developed.The maximum impact velocity could reach 324 km/h.They can be used to induce impact injuries on different animals,at different patterns and even on different regions of body.7.The inflicting mechanisms for cranioserebral and chest impact injuries were investigated.Conclusions:1.With the rapid development of the economy and sharp increase of motor vehicles in China,RTAs also accordingly increase.The traffic administration and road construction,although being greatly improved,are still behind the increase of motor vehicles,which contradiction is still difficults resolved to update.2.In the next centrury,we should establish national database of traffic injuries,improve national emergency medical services system(EMSS)and the safety consciousness of road users,and comprehensively govern RTAs and traffic accident injuries.

Protective effects of fibronectin on vascular endothelial cells during trauma in rats

Objective To investigate the Protectiveeffects of fibronectin(Fn)on vascular endo-thelial cells. Methods Block reticuloendothelial sys-tem model compound with injury of tail veinand femur fractures in rats were used.Thelevel of plasma Fn and surface Fn of peritonealmacrophage cells,the numbers of circulatingendothelial cells and the injured tail vein wereobserved.