Apple of Sodom (<em>Calatropis procera</em>) Callus Extract, a Novel Skincare Active and Its Biological Activity in Skin Models When Combined with Dead Sea Water

Background: Calotropis procera (C. procera), is an authentic plant naturally grown in the flora of Dead Sea region. Despite its toxicity, C. procera presents healing properties. However, it has not been implemented yet in cosmetics as an active ingredient. Objective: The biological effects of C. procera callus extract on skin were elucidated solely and in combination with Dead Sea water (DSW). Methods: The capability of C. procera extract to protect against skin inflammation and irritation was tested on ex vivo human skin organ culture by LPS and SDS addition respectively. Viability and cytokine secretion were evaluated. The combination of C. procera extract with Dead Sea water was tested on full thickness skin equivalents. Gene expression and relevant biochemical markers for glycolysis, hypoxia and extracellular matrix balance were tested. Results: C. procera extract exhibits a protective biological activity against skin irritation and inflammation at the biochemical level. Furthermore, a combination of C. procera extract and DSW demonstrates a potential contribution for skin wellbeing via enhance energy production, resistance to hypoxia and extracellular matrix balance. Conclusions: Topical application of C. procera callus extract might support skin balance and wellbeing at the molecular level. Hence, it is recommended for new cosmetic formulae as standalone or in combination with Dead Sea water, in the effort to achieve anti-aging bio-activity that is working beyond skin aging symptoms, especially via skin calming effects and skin energy enhancement.

Dead Sea Minerals-Induced Positive Stress as an Innovative Resource for Skincare Actives

Objective: Exposure to certain stresses in small doses might lead to a protective effect by improving resistance to other stressors. Dead Sea (DS) minerals can be a relevant source to induce positive stress due to their high salinity and unique mineral combination. This concept could be further optimized using advanced unique cell biotechnology. The purpose of this study was to elucidate the innovative concept of DS minerals (water extract and black mud) supplementation in small amount to Pichia pastoris yeast growth media as a positive stress by testing the capability of accepted fermentation compounds to affect the appearance of skin. Methods: Skin equivalents were topically applied with different Pichia pastoris fermentations (Metabiotics?). Skin elasticity biomarkers were tested, since loss of elasticity and suppleness is a natural skin aging process leading to deeper wrinkles and loss of firmness. A preliminary screening at the gene level using DNA microarray was performed and subsequently, the following proteins were detected using ELISA or immunoblotting assays: elastin, fibulin-1, lysyl oxidase (LOX), metalloproteinase 3 (MMP-3), E-cadherin, claudin 4, tight junction protein (TJP)-1 and TJP-2. UVB irradiation was selected as a stressor. Results: Fermentation compounds generated in the presence of small doses of DS minerals affected the expression of various elasticity-related genes in skin. Moreover, they significantly attenuated the abnormal UVB-induced alterations, the proteins elastin, fibulin-1, LOX, MMP-3, E-cadherin and TJP-2. Conclusions: The observations clearly demonstrate that when DS Metabiotics? compounds are topically applied, significant alterations in several biomarkers that contribute to skin elasticity occur. Thus, these novel compounds have the potential to serve as skincare actives.

An arthropod community beyond the dry limit of plant life

Water availability,which enables plant growth and animal activity,regulates dryland ecosystem function.In hyper-arid ecosystems,rain cannot support vascular plant growth.Therefore,hyper-arid vegetation is restricted to the lower topography,where runoff accumulates.Typically,food resources originating from areas of dense vegetation are dispersed across the desert floor,enabling animal life in areas lacking vascular plant growth.However,certain regions,such as the hyper-arid upper topography,may be devoid of plant-derived food resources.The present study examined arthropod activity in the upper topography of a hyper-arid desert,in comparison with arthropod activity in the lower topography.Pitfall traps were utilized to compare arthropod activity along unvegetated ridges with activity in parallel,vegetated riverbeds.Surprisingly,the study revealed dense arthropod communities in the barren upper topography.Arthropods collected in the upper topography represented 26%of total arthropod abundance.In addition,the overlap between arthropod identity in the ridges and wadis(i.e.,riverbeds)was low,and certain arthropods were strongly affiliated with the ridges.The upper topographic communities included high numbers of silverfish(Zygentoma:Lepismatidae),malachite beetles(Psiloderes),and predatory mites(Acari:Anystidae),and these arthropods were present at various life stages.It remains unclear how arthropod communities can persist in the unvegetated upper topography of the hyper-arid study area.These results raise the possibility that other food sources,independent from vascular plants,may play a significant role in the life history of hyper-arid arthropods.

How to Predict AGEs Accumulation Slowdown Effect of a Cosmetic Ingredient? Two Steps <i>In-Vitro</i>System for Evaluating the Anti-AGE Impact of a New Blend

Advanced Glycation End-Products (AGEs), play a crucial part in advancing the process of cellular skin aging and its link to chronological age was re-assessed. AGEs accumulation alters cell structure and function of most types of skin cells, affecting skin’s mechanical and physiological properties, following the molecular transformations. Slowdown AGEs accumulation rate in skin, although a potent anti-aging strategy, is difficult and tricky. The lack of working methods for In-Vitro and In-Vitro measuring AGEs level complicates the evaluation and prediction of active ingredients’ ability to affect cellular AGEs accumulation. A two-step In-Vitro systematic screening method is proposed and three different cosmetic active ingredients were selected for its demonstration, using BSA-Glucose and Collagen-Glucose predicting models. Candidates’ effects on AGEs accumulation were evaluated as standalone, and when formulated in a blend. Additionally, the potency of non-invasive auto-fluorescence in-vivo measurement to detect AGEs levels among subjects of different ages was demonstrated. The results are presented in this work and the potential contribution of the proposed system to assist the desired inhibition of AGEs accumulation in skin is discussed.

以色列严重退化旱地长期恢复对土壤和植被的影响

Land degradation affects extensive drylands around the world.Due to long-term misuse,the Israeli Sde Zin dryland site has faced severe degradation.The study objective was to assess the feasibility of passive restoration in recovering the site.The study was conducted in four land-units along a preservation-degradation continuum:(1)an area that has not faced anthropogenic disturbances(Ecological land);(2)an area that was proclaimed as a national park in the 1970s(Rehabilitation);(3)an area that was prone,until recently,to moderate anthropogenic pressures(Triangle);and(4)a dirt road that was subjected to long-term off-road traffic(Dirtroad).Soil was sampled and analyzed for its properties.The soil physical quality followed the trend of Ecological land>Rehabilitation>Triangle>Dirtroad.Specifically,high soil salinity in the latter three land-units is attributed to long-term erosional processes that exposed the underlying salic horizons.Herbaceous and shrubby vegetation cover was also monitored.The herbaceous vegetation cover followed the trend of Ecological land(86.4%)>Rehabilitation(40.3%)>Triangle(26.2%)>Dirtroad(2.1%),while the shrubby cover was 2.8%in the Ecological land-unit,and practically zero in the other land-units.It seems that despite the effectiveness of passive restoration in recovering the soil’s physical properties,the recovery of vegetation is limited by the severe soil salinity.

枯死灌木斑块对退化旱地生态系统的调节作用

A long-term drought has led to the mass mortality of shrubs in the semi-arid Israeli Negev.The most impacted shrub species is the Noaea mucronata(Forssk.)Asch.and Schweinf.In a four-year study,we found that herbaceous vegetation growth was greater in the dead shrub patches than in the surrounding inter-patch biocrusted spaces,suggesting that the dead shrub patches encompass improved micro-habitats.However,unexpectedly,the soil moisture in the dead shrub patches was consistently lower than that of the inter-patch biocrusted spaces.At the same time,soil quality in the dead shrub patches was higher than that in the inter-patch spaces.Therefore,it seems that the overall better soil conditions in the dead patches overcome the scarcity of soil-water,supporting increased herbaceous productivity.For explaining the discrepancy between herbaceous vegetation and soil-water,we formulated a conceptual framework,which highlights the key factors that regulate soil-water dynamics in this dryland ecosystem.We demonstrate that herbaceous vegetation is facilitated in the dead shrub patches by a legacy effect that takes place long after the shrubs have died.The dead shrub patches encompass a unique form of ecosystem engineering.The study highlights the complex and unpredicted impacts of prolonged droughts on dryland ecosystems.