Context: Anesthetic tubes are always on the surgical table, for this reason, it's necessary to perform its disinfection, generally by chemicals. Aim: Was to analyze the effectiveness of tubes decontamination made b...Context: Anesthetic tubes are always on the surgical table, for this reason, it's necessary to perform its disinfection, generally by chemicals. Aim: Was to analyze the effectiveness of tubes decontamination made by disinfectant substances used in a clinical setting and to identify the microorganisms found on their surface. Materials and Methods: 14 anesthetic tubes were collected from dental clinic, on 5 groups. 01 plastic and 01 glass tubes that were not submitted to any disinfection process composed the control group and they had been sown in Agar Blood. Experimental groups underwent immersion and fixation with the proposed chemicals for 1 minute, laminar flow hood was used to seed it in Agar Blood and then it was transferred to incubator for 24 hours at 37℃. Tests were used to identify the main Gram positive pathogenic genera. Gram-negative pathogenic colonies isolated were seeded in biochemical means of identification. Results: It was possible to identify the presence of Staphylococcus coagulase negative, Staphylococcus aureus, Streptococcus viridans, Staphylococcus saprophyticus, Escherichia coli and Acinetobacter spp, as well as the effectiveness of chemical solutions proposed in this study. Conclusions: All chemical solutions proposed in this study were effective for the surface disinfection process of anesthetic tubes.展开更多
Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascular...Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)nanofibers.Based on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory activities.The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth fragments.Thus,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.展开更多
文摘Context: Anesthetic tubes are always on the surgical table, for this reason, it's necessary to perform its disinfection, generally by chemicals. Aim: Was to analyze the effectiveness of tubes decontamination made by disinfectant substances used in a clinical setting and to identify the microorganisms found on their surface. Materials and Methods: 14 anesthetic tubes were collected from dental clinic, on 5 groups. 01 plastic and 01 glass tubes that were not submitted to any disinfection process composed the control group and they had been sown in Agar Blood. Experimental groups underwent immersion and fixation with the proposed chemicals for 1 minute, laminar flow hood was used to seed it in Agar Blood and then it was transferred to incubator for 24 hours at 37℃. Tests were used to identify the main Gram positive pathogenic genera. Gram-negative pathogenic colonies isolated were seeded in biochemical means of identification. Results: It was possible to identify the presence of Staphylococcus coagulase negative, Staphylococcus aureus, Streptococcus viridans, Staphylococcus saprophyticus, Escherichia coli and Acinetobacter spp, as well as the effectiveness of chemical solutions proposed in this study. Conclusions: All chemical solutions proposed in this study were effective for the surface disinfection process of anesthetic tubes.
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnologico(CNPq)(409196/2018-5),Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior(CAPES)(88887.202222/2018-00)Fundaçao de Apoioa Pesquisa do Distrito Federal(FAPDF)(00193-00000782/2021-63),and Fundaçao de Apoio ao Desenvolvimento do Ensino,Ciˆencia e Tecnologia do Estado de Mato Grosso do Sul(FUNDECT)(59/300.397/2015+2 种基金022/2018028973)In addition,this study was supported by a Canadian Institutes of Health Research Foundation grant FDN-154287 to R.E.W.Hancock.HE is the recipient of a UBC Killam Fellowship and a Research Trainee Award from the Michael Smith Foundation for Health Research(MSFHR).R.E.W.Hancock is a Canada Research Chair in Health and Genomics and a UBC Killam Professor.We also acknowledge Professor Marcelo Oliveira Rodrigues and Chemistry Institute(University of Brasilia)for all support.
文摘Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)nanofibers.Based on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory activities.The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth fragments.Thus,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.