The iodination efficiency of salmon GH(sGH) was 38.82%,using a modification of the chloramine T method. The specific activity of the 125 I sGH was about 40 μCi/μg protein. The results of binding assay showed a singl...The iodination efficiency of salmon GH(sGH) was 38.82%,using a modification of the chloramine T method. The specific activity of the 125 I sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low capacity binding site in flounder liver. Long term administration with exogenous GH can induce the up regulation of hepatic GH receptor in total binding capacity though there was no significant difference of association constant among any groups. Considering that there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites.展开更多
文摘The iodination efficiency of salmon GH(sGH) was 38.82%,using a modification of the chloramine T method. The specific activity of the 125 I sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low capacity binding site in flounder liver. Long term administration with exogenous GH can induce the up regulation of hepatic GH receptor in total binding capacity though there was no significant difference of association constant among any groups. Considering that there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites.
