Objectives:To determine whether air pollution or changes in SARS-CoV-2 lineages lead to an increase in mortality.Methods:Descriptive statistics were used to calculate rates of infection(2020-2021).RT ePCR was used to ...Objectives:To determine whether air pollution or changes in SARS-CoV-2 lineages lead to an increase in mortality.Methods:Descriptive statistics were used to calculate rates of infection(2020-2021).RT ePCR was used to compare viral loads from October 2020 to February 2021.Nextgeneration sequencing(NGS)(n=92)was used to examine and phylogenetically map SARS-CoV-2 lineages.A correlative“air pollution/temperature”index(I)was developed using regression analysis.PM_(2.5),PM_(10),O_(3),NO_(2),SO_(2),and CO concentrations were analyzed and compared to the mortality.Results:The mortality rate during the last year was~32%.Relative SARS-CoV-2 viral loads increased in December 2020 and January 2021.NGS revealed that approximately 80%of SARS-CoV-2 linages were B.1.243(33.7%),B1.1.222(11.2%),B.1.1(9%),B.1(7%),B.1.1.159(7%),and B.1.2(7%).Two periods were analyzed,the prehigh-and high-mortality periods and no significant lineage differences or new lineages were found.Positive correlations of air pollution/temperature index values with mortality were found for IPM_(2.5) and IPM_(10).INO_(2).ISO_(2),and ICO but not for O_(3).Using ICO,we developed a model to predict mortality with an estimated variation of~±5 deaths per day.展开更多
基金funded by Mexicana de Investigacion y Biotectogía,S.A.de C.V.
文摘Objectives:To determine whether air pollution or changes in SARS-CoV-2 lineages lead to an increase in mortality.Methods:Descriptive statistics were used to calculate rates of infection(2020-2021).RT ePCR was used to compare viral loads from October 2020 to February 2021.Nextgeneration sequencing(NGS)(n=92)was used to examine and phylogenetically map SARS-CoV-2 lineages.A correlative“air pollution/temperature”index(I)was developed using regression analysis.PM_(2.5),PM_(10),O_(3),NO_(2),SO_(2),and CO concentrations were analyzed and compared to the mortality.Results:The mortality rate during the last year was~32%.Relative SARS-CoV-2 viral loads increased in December 2020 and January 2021.NGS revealed that approximately 80%of SARS-CoV-2 linages were B.1.243(33.7%),B1.1.222(11.2%),B.1.1(9%),B.1(7%),B.1.1.159(7%),and B.1.2(7%).Two periods were analyzed,the prehigh-and high-mortality periods and no significant lineage differences or new lineages were found.Positive correlations of air pollution/temperature index values with mortality were found for IPM_(2.5) and IPM_(10).INO_(2).ISO_(2),and ICO but not for O_(3).Using ICO,we developed a model to predict mortality with an estimated variation of~±5 deaths per day.
