Lavender （L. angustifolia） is a genus of flowering plants in the mint family, Lamiaceae. It is an aromatic shrub with multiple stems growing 30 to 60 cm tall. It is native to Asia, Europe and Mediterranean regions a...Lavender （L. angustifolia） is a genus of flowering plants in the mint family, Lamiaceae. It is an aromatic shrub with multiple stems growing 30 to 60 cm tall. It is native to Asia, Europe and Mediterranean regions and grows on hilly areas and coastlines. The evergreen leaves are narrow and long and covered in fine hairs, which normally contain the essential oils. The leaves are also pinnately toothed, or pinnate. The plant blooms between April and June, and flowers are purple produced on violet bracts. All parts of the plant have a strong and scented odor and a bitter taste. The flowers and leaves are used as an herbal medicine. Lavender essential oil is extracted by distilling flowers and leaves, Essential oil has higher levels of linalyl acetate （40%）, butyric acid, propionic acid, valeric acid, free linalool. Considering the lack of information about lavender tissue culture, the current experiment＇ was aimed to study the effect of phytohormones on lavender callus induction and organogenesis. The lavender explants （leaf and apical meristem） were cultured onto MS media supplemented with different phytohormones. According to the observations, 5 mg/L NAA and 5 mg/L KIN treatment caused leaf and root formation, which were originally derived t^om dense and green calli. The MS medium containing 5 mg/L IBA and 5 mg/L Kin was effective just on leafy explants so that explants formed into leaves after callus induction. Application of 5 mg/L NAA and 5 mg/L BAP on apical meristem explants produced shoot and root after callus induction. However, complete plants were produced after sub-culturing the samples. In conclusion, MS medium supplemented with 5 mg/L NAA and 5 mg/L BAP was selected as the best medium for lavender tissue culture.展开更多
文摘Lavender （L. angustifolia） is a genus of flowering plants in the mint family, Lamiaceae. It is an aromatic shrub with multiple stems growing 30 to 60 cm tall. It is native to Asia, Europe and Mediterranean regions and grows on hilly areas and coastlines. The evergreen leaves are narrow and long and covered in fine hairs, which normally contain the essential oils. The leaves are also pinnately toothed, or pinnate. The plant blooms between April and June, and flowers are purple produced on violet bracts. All parts of the plant have a strong and scented odor and a bitter taste. The flowers and leaves are used as an herbal medicine. Lavender essential oil is extracted by distilling flowers and leaves, Essential oil has higher levels of linalyl acetate （40%）, butyric acid, propionic acid, valeric acid, free linalool. Considering the lack of information about lavender tissue culture, the current experiment＇ was aimed to study the effect of phytohormones on lavender callus induction and organogenesis. The lavender explants （leaf and apical meristem） were cultured onto MS media supplemented with different phytohormones. According to the observations, 5 mg/L NAA and 5 mg/L KIN treatment caused leaf and root formation, which were originally derived t^om dense and green calli. The MS medium containing 5 mg/L IBA and 5 mg/L Kin was effective just on leafy explants so that explants formed into leaves after callus induction. Application of 5 mg/L NAA and 5 mg/L BAP on apical meristem explants produced shoot and root after callus induction. However, complete plants were produced after sub-culturing the samples. In conclusion, MS medium supplemented with 5 mg/L NAA and 5 mg/L BAP was selected as the best medium for lavender tissue culture.
