Overexpression of Protein Phosphatase 2 Regulatory Subunit B"Alpha Promotes Glycolysis by Regulating Hexokinase 1 in Hepatocellular Carcinoma

Objective To investigate the regulatory relationship of Protein Phosphatase 2 Regulatory Subunit B"Alpha(PPP2R3A)and hexokinase 1(HK1)in glycolysis of hepatocellular carcinoma(HCC).Methods In HepG2 and Huh7 cells,PPP2R3A expression was silenced by small interfering RNA(siRNA)and overexpression by plasmid transfection.The PPP2R3A-related genes were searched by RNA sequencing.Glycolysis levels were measured by glucose uptake and lactate production.QRT-PCR,ELISA,western blot and immunofluorescence assay were performed to detect the changes of PPP2R3A and HK1.Cell proliferation,migration and invasion assay were used to study the roles of HK1 regulation by PPP2R3A.Results RNA sequencing data revealed that PPP2R3A siRNA significantly downregulated the expression of HK1.PPP2R3A gene overexpression promotes,while gene silencing suppresses,the level of HK1 and glycolysis in HCC cells.In HCC tissue samples,PPP2R3A and HK1 were colocalized in the cytoplasm,and their expression showed a positive correlation.HK1 inhibition abrogated the promotion of glycolysis,proliferation,migration and invasion by PPP2R3A overexpression in liver cancer cells.Conclusion Our findings showed the correlation of PPP2R3A and HK1 in the glycolysis of HCC,which reveals a new mechanism for the oncogenic roles of PPP2R3A in cancer.

Development and Validation of an Automatic Ultrawide-Field Fundus Imaging Enhancement System for Facilitating Clinical Diagnosis:A Cross-Sectional Multicenter Study

In ophthalmology,the quality of fundus images is critical for accurate diagnosis,both in clinical practice and in artificial intelligence(AI)-assisted diagnostics.Despite the broad view provided by ultrawide-field(UWF)imaging,pseudocolor images may conceal critical lesions necessary for precise diagnosis.To address this,we introduce UWF-Net,a sophisticated image enhancement algorithm that takes disease characteristics into consideration.Using the Fudan University ultra-wide-field image(FDUWI)dataset,which includes 11294 Optos pseudocolor and 2415 Zeiss true-color UWF images,each of which is rigorously annotated,UWF-Net combines global style modeling with feature-level lesion enhancement.Pathological consistency loss is also applied to maintain fundus feature integrity,significantly improving image quality.Quantitative and qualitative evaluations demonstrated that UWF-Net outperforms existing methods such as contrast limited adaptive histogram equalization(CLAHE)and structure and illumination constrained generative adversarial network(StillGAN),delivering superior retinal image quality,higher quality scores,and preserved feature details after enhancement.In disease classification tasks,images enhanced by UWF-Net showed notable improvements when processed with existing classification systems over those enhanced by StillGAN,demonstrating a 4.62%increase in sensitivity(SEN)and a 3.97%increase in accuracy(ACC).In a multicenter clinical setting,UWF-Net-enhanced images were preferred by ophthalmologic technicians and doctors,and yielded a significant reduction in diagnostic time((13.17±8.40)s for UWF-Net enhanced images vs(19.54±12.40)s for original images)and an increase in diagnostic accuracy(87.71%for UWF-Net enhanced images vs 80.40%for original images).Our research verifies that UWF-Net markedly improves the quality of UWF imaging,facilitating better clinical outcomes and more reliable AI-assisted disease classification.The clinical integration of UWF-Net holds great promise for enhancing diagnostic processes and patient care in ophthalmology.

瑞典交互性阈值快速计算视野检查在青春期前特发性颅内高压中的应用

Objective To examine the reliability of Swedish Interactive Thresholding Algor ithm Fast (SITA Fast) visual fields (VFs) in prepubertal idiopathic intracranial hypertension (IIH) and to compare whether age, gender, or severity of visual outcome influenced the reliability o f VF tests. Design Prospective, longitudinal cohort study. Participants Twenty- six prepubertal children (< 11 years of age; mean age, 7.2 years) with IIH. Test ing Children were prospectively followed up using a childoriented program, which included SITA Fast VF tests. Age, gender, and severity of visual outcome were c orrelated with reliability of performance on SITA Fast VFs using a 1-way analys is of variance, point-biserial correlation, and the chi-square test for indepe ndence of observation. Main outcome measures Statistical analyses results that c orrelated the reliability of SITA Fast VFs with age, gender, and visual outcome. Results Three children were treated at the age of 2 years and were unable to pe rform automated VF tests. Four children were treated at the age of 4 years, 3 of whom were able to perform repeatable reliable SITA Fast VF tests. Forty-two ey es of 21 children had an average SIT AFast test time of 4.5 minutes for each eye , compared with 8 minutes using the Fastpac strategy. Age was not associated wit h reliability scores of SITA Fast tests (F=0.971, not significant ns). Gender did not influence the reliability of SITA Fast VF tests (chi-square(1)=0.669, n s), nor did severity of visual outcome (chi-square(2)=3.348, ns). Visual defici ts were observed in 55%of patients at presentation and in 27%of patients after resolution of papilledema. Conclusions The SITA Fast VF tests can be performed from age 4 years and offer a reliable method for shortening test time. A child- oriented follow-up program, which entails a shortened testing time, may improve outcome in prepubertal IIH.

四点巩膜固定法用于后房型人工晶状体植入术时预防缝线结暴露

BACKGROUND AND OBJECTIVE: The results and complications of posterior chamber i ntraocular lens (IOL) implantation by a 4-point scleral fixation technique are described. PATIENTS AND METHODS: Fifty eyes of 47 patients who underwent scleral -fixated IOL implantation were retrospectively evaluated. Twenty-one (42%) ey es had a history of trauma and 29 (58%) eyes had previously undergone cataract surgery. In all cases, IOL implantation by 4-point scleral fixation was perform ed and the knots of fixation sutures were rotated and buried in the globe. The I OL position was adjusted by suture rotation for best centration. RESULTS: The me an follow-up time was 7 ±4 months. Four (8%) eyes had minimal corneal edema p reoperatively. Cystoid macular edema was noted in 2 (6.8%) eyes in the cataract surgery group and 8 (38%) eyes in the posttraumatic group. Two (9.5%) eyes in the posttraumatic group had atrophic macular changes and 1 (4.7%) had corneal scarring,which impaired vision. No complications such as knot exposure, tilting of the IOL, decentralization, or endophthalmitis were noted postoperatively. Pos toperative mean corrected visual acuity was 0.4 ±0.3 in the posttraumatic group and 0.4 ±0.2 in the cataract surgery group. CONCLUSION: The 4-point scleral f ixation technique resulted in no serious postoperative complications such as sut ure exposure and endophthalmitis. Because the knot can be rotated and buried in the globe, knot exposure is less likely to occur. This procedure ismore effectiv e than other techniques regarding IOL centralization.

光学相干断层成像及共焦激光扫描断层成像在评估黄斑水肿中的应用

Purpose To compare optical coherence tomography (OCT) and confocal scanning la ser tomography (cSLT) for quantitative retinal thickness mapping of the macula a nd their ability to detect macular edema. Design Prospective, comparative, clini cal observational study. Methods The study population of 138 eyes (97 patients) was divided into a study group consisting of 45 (32.6%) eyes with macular edema and a control group consisting of 93 (67.4%) eyes without macular edema. All p atients underwent OCT and cSLT of the macula. Retinal thickness measurements obt ained by OCT were compared with signal width and edema index, determined by cSLT . Results The OCT measurements and cSLT edema index were significantly (P< 0 .00 1) correlated with each other. Correlation coefficients decreased (P< 0.001)with increasing diameter of the measurement circle. In the macular edema group, corr elation coefficients were significantly (P< 0.001) higher than in the control gr oup. To separate the study and control groups, receiver operator characteristic curves covered a larger area for OCT measurements than for cSLT measurements. Re tinal thickness measurements and edema index correlate with visual acuity (corre lation coefficient r=-0.653 for OCT, r=-0.608 for cSLT; P < .001). Conclusions Macular edema can be quantitatively mapped by OCT and cSLT. The retinal thickne ss and edema index measurements correlate with visual acuity. The fast and stand ard examination modes of OCT give similar measurements. Both OCT and cSLT can di fferentiate between eyes with and without macular edema, with OCT showing a high er predictive value.