Herein, we described the physicochemical properties of deproteinized extract of calf blood(DECB) and established a hypoxia model treated with or without DECB to detect the sugar, lactic acid, protein, and ATP contents...Herein, we described the physicochemical properties of deproteinized extract of calf blood(DECB) and established a hypoxia model treated with or without DECB to detect the sugar, lactic acid, protein, and ATP contents of mice and then identified and analyzed the differentially expressed genes between two groups using mRNA expression chip. According to the results of the airtight hypoxia experiment, mice in the model+DECB group had a signifi? cantly prolonged time of hypoxia tolerance compared with the model group. The biochemical test indicated that DECB could significantly increase the level of sugar, ATP and proteins and reduce the amount of lactic acid in mice. It also revealed that Hmgcs2, Cptla, Angptl4, Cyp8b], and Ehhadh genes were involved in mice energy metabolism, and were closely associated witli metabolic signaling pathway. These results suggest that DECB might be a potential drug to treat metabolic diseases. Among the genes with differential expression under hypoxia, Angptl4, Cyp8bl, and Ehhadh were critical factors for sugar metabolism. Hmgcs2 provided energy directly, and Cptla regulated cellular inflammatory responses, promoting energy metabolism.展开更多
基金the Natural Science Foundation of Jilin Province, China(No.20180101099JC)the "Thirteen Five"Science and Technology Project of the Department of Education of Jilin Province, China(No.JJKH20180372KJ).
文摘Herein, we described the physicochemical properties of deproteinized extract of calf blood(DECB) and established a hypoxia model treated with or without DECB to detect the sugar, lactic acid, protein, and ATP contents of mice and then identified and analyzed the differentially expressed genes between two groups using mRNA expression chip. According to the results of the airtight hypoxia experiment, mice in the model+DECB group had a signifi? cantly prolonged time of hypoxia tolerance compared with the model group. The biochemical test indicated that DECB could significantly increase the level of sugar, ATP and proteins and reduce the amount of lactic acid in mice. It also revealed that Hmgcs2, Cptla, Angptl4, Cyp8b], and Ehhadh genes were involved in mice energy metabolism, and were closely associated witli metabolic signaling pathway. These results suggest that DECB might be a potential drug to treat metabolic diseases. Among the genes with differential expression under hypoxia, Angptl4, Cyp8bl, and Ehhadh were critical factors for sugar metabolism. Hmgcs2 provided energy directly, and Cptla regulated cellular inflammatory responses, promoting energy metabolism.
