Mice carrying mutant amyloid precursor protein and presenilin-1 genes (APP/PS1 double trans- genic mice) have frequently been used in studies of Alzheimer's disease; however, such studies have focused mainly on hip...Mice carrying mutant amyloid precursor protein and presenilin-1 genes (APP/PS1 double trans- genic mice) have frequently been used in studies of Alzheimer's disease; however, such studies have focused mainly on hippocampal and cortical changes. The severity of Alzheimer's disease is known to correlate with the amount of amyloid-13 protein deposition and the number of dead neurons in the locus coeruleus. In the present study, we assigned APP/PS1 double transgenic mice to two groups according to age: young mice (5-6 months old) and aged mice (16-17 months old). Age-matched wild-type mice were used as controls. Immunohistochemistry for tyrosine hydroxylase (a marker of catecholaminergic neurons in the locus coeruleus) revealed that APP/PS1 mice had 23% fewer cells in the locus coeruleus compared with aged wild-type mice. APP/PS1 mice also had increased numbers of cell bodies of neurons positive for tyrosine hydroxylase, but fewer tyrosine hydroxylase-positive fibers, which were also short, thick and broken. Quantitative analysis using unbiased stereology showed a significant age-related increase in the mean volume of tyrosine hy- droxylase-positive neurons in aged APP/PS1 mice compared with young APP/PS1 mice. Moreover, the mean volume of tyrosine hydroxylase-positive neurons was positively correlated with the total volume of the locus coeruleus. These findings indicate that noradrenergic neurons and fibers in the locus coeruleus are predisposed to degenerative alterations in APP/PS1 double transgenic mice.展开更多
The limited ability for injured adult axons to regenerate is a major cause for limited functional recovery after injury to the nervous system,motivating numerous efforts to uncover mechanisms capable of enhancing rege...The limited ability for injured adult axons to regenerate is a major cause for limited functional recovery after injury to the nervous system,motivating numerous efforts to uncover mechanisms capable of enhancing regeneration potential.One promising strategy involves deletion or knockdown of the phosphatase and tensin(PTEN) gene.Conditional genetic deletion of PTEN before,immediately following,or several months after spinal cord injury enables neurons of the corticospinal tract(CST) to regenerate their axons across the lesion,which is accompanied by enhanced recovery of skilled voluntary motor functions mediated by the CST.Although conditional genetic deletion or knockdown of PTEN in neurons enables axon regeneration,PTEN is a well-known tumor suppressor and mutations of the PTEN gene disrupt brain development leading to neurological abnormalities including macrocephaly,seizures,and early mortality.The long-term consequences of manipulating PTEN in the adult nervous system,as would be done for therapeutic intervention after injury,are only now being explored.Here,we summarize evidence indicating that long-term deletion of PTEN in mature neurons does not cause evident pathology; indeed,cortical neurons that have lived without PTEN for over 1 year appear robust and healthy.Studies to date provide only a first look at potential negative consequences of PTEN deletion or knockdown,but the absence of any detectable neuropathology supports guarded optimism that interventions to enable axon regeneration after injury are achievable.展开更多
Studies on a variety of highly regenerative tissues, including the central nervous system(CNS) in non-mammalian vertebrates, have consistently demonstrated that tissue damage induces the formation of an ionic curren...Studies on a variety of highly regenerative tissues, including the central nervous system(CNS) in non-mammalian vertebrates, have consistently demonstrated that tissue damage induces the formation of an ionic current at the site of injury. These injury currents generate electric fields(EF) that are 100-fold increased in intensity over that measured for uninjured tissue. In vitro and in vivo experiments have convincingly demonstrated that these electric fields(by their orientation, intensity and duration) can drive the migration, proliferation and differentiation of a host of cell types. These cellular behaviors are all necessary to facilitate regeneration as blocking these EFs at the site of injury inhibits tissue repair while enhancing their intensity promotes repair. Consequently, injury-induced currents, and the EFs they produce, represent a potent and crucial signal to drive tissue regeneration and repair. In this review, we will discuss how injury currents are generated, how cells detect these currents and what cellular responses they can induce. Additionally, we will describe the growing evidence suggesting that EFs play a key role in regulating the cellular response to injury and may be a therapeutic target for inducing regeneration in the mammalian CNS.展开更多
Objective: To investigate the distribution and contents of vimentin(Vim) and glial fibrillary acidic protein(GFAP) immunoreactivities in the central nervous system(CNS)of normal newborn, adult and aged rats.Methods: I...Objective: To investigate the distribution and contents of vimentin(Vim) and glial fibrillary acidic protein(GFAP) immunoreactivities in the central nervous system(CNS)of normal newborn, adult and aged rats.Methods: In this study, thirty healthy and normal Sprague–Dawley rats were simply classified into three groups: Newborn(7 days aged), adult(5 months aged) and aged(24 months aged) rats. Brains and spinal cord were dissected and cut into frozen sections. The expression of Vim and GFAP in CNS were detected by confocal immunofluorescence.Results: In each group, Vim was expressed in all the regions of CNS including the hippocampal, cerebral cortex, the third ventricle and spinal cord, and the expression was highest in neuron-like cell of newborn rats, while Vim was mainly expressed in cell bodies in adult and aged rats. GFAP was expressed in all the regions of CNS including the hippocampal, cerebral cortex, the third ventricle and spinal cord, and the expression was in astrocytes of aged rats. In the third ventricle, Vim was detected in all groups, and only observed in neuron-like cells of newborn. Meanwhile, the GFAP expression showed no significant differences between adult and aged rats in this region. The co-localization of Vim and GFAP were mainly observed in hippocampus and cerebral cortex of newborn,but this co-localization was found in the third ventricle of the rats in all groups.Conclusion: Our data demonstrate for the first time that the expression of Vim and GFAP in the rat's CNS during development. This data may provide a foundation for the further mechanistic studies of these two main intermediate filaments during development of CNS.展开更多
BACKGROUND: Exogenous brain-derived neurotrophic factor (BDNF) promotes retinal ganglion cell survival. However, the protective mechanisms remain unclear. OBJECTIVE: To investigate changes in retinal tyrosine kina...BACKGROUND: Exogenous brain-derived neurotrophic factor (BDNF) promotes retinal ganglion cell survival. However, the protective mechanisms remain unclear. OBJECTIVE: To investigate changes in retinal tyrosine kinase receptor B (trkB) expression and effects of exogenous BDNF on trkB activation in a rat model of acute high intraocular pressure (HtOP). DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Department of Anatomy and Neurobiology, Xiangya Medical School, Central South University from January 2004 to August 2006. MATERIALS: Rabbit anti-BDNF and anti-trkB.FL(full-length) polyclonal antibodies were purchased from Santa Cruz Biotechnology, USA; rabbit anti-p-trkB polyclonal antibodies were purchased from Cellsignal, USA. METHODS: A total of 48 healthy, adult, Sprague Dawiey rats were randomly assigned to acute HIOP (without BDNF pre-treatment) and BDNF pre-treated groups, with 24 animals in each group. In the BDNF pre-treated group, the left eyes were intravitreally injected with 3 pg/kg BDNF 2 days prior to HIOP. Rats in the acute HIOP group were not pre-treated with BDNE HIOP models were established by increased intraocular pressure in the left eyes until the b-wave of flash electroretinogragh disappeared and pressure was maintained for 60 minutes. The right eyes of all rats were not treated and served as the normal controls. MAIN OUTCOME MEASURES: Retinal structure and cell numbers in the ganglion cell layer (GCL) were detected by Nissl staining; expression of trkB and phosphorylated trkB in the rat retina were determined by immunohistochemistry. RESULTS: A greater number of GCL neurons were observed in the pre-treated group compared to the acute HIOP group (P 〈 0.05). TrkB expression was significantly increased following HIOP at days 1 and 3 (P 〈 0.05), but expression varied between retinal areas. Although trkB expression decreased at 7 days, phosphorylated trkB dramatically decreased with increasing time (P 〈 0.05). TrkB expression in BDNF pre-treated rats was similar to the acute HIOP group at early injury time points. Nevertheless, trkB expression was significantly decreased compared to the acute HIOP group at 7 days (P 〈 0.05), and phosphorylated trkB expression was significantly greater compared to the acute HIOP group at each time point (P〈 0.05). CONCLUSION: TrkB expression displayed temporal and spatial changes in the rat retina following acute HIOP, and trkB up-regulation suggested that more BDNF was required for treating the injured retina. Exogenous BDNF partially ameliorated decreased expression of phosphorylated trkB and provided protection to the injured retina, to a certain degree, following HIOP.展开更多
基金National Natural Science Foundation of China (39925018, 90508002 , 30121001) Chinese Academy of Science (KSCX 1-R65 and RSCX2-H10)+2 种基金 National Basic Research Program of China (973 project, 2002CB713700) American Cancer Society (RPG-99-173-01) a Gcc Breast Cancer Research award and National Institutes of Health grants DK56292 and CA89019 to XY (a GCC Eminent Scholar) and NS36194 (JW).
基金supported by the National Natural Science Foundation of China, No. 81100663the Scientific Research Funds of the Health Department of Hunan Province, No.120303+1 种基金Hunan Provincal Natural Science Foundation of China, No. 13JJ3058a grant from the Scientific Research Program of Hunan Provincial Higher Education Institutes, No. 11C0829
文摘Mice carrying mutant amyloid precursor protein and presenilin-1 genes (APP/PS1 double trans- genic mice) have frequently been used in studies of Alzheimer's disease; however, such studies have focused mainly on hippocampal and cortical changes. The severity of Alzheimer's disease is known to correlate with the amount of amyloid-13 protein deposition and the number of dead neurons in the locus coeruleus. In the present study, we assigned APP/PS1 double transgenic mice to two groups according to age: young mice (5-6 months old) and aged mice (16-17 months old). Age-matched wild-type mice were used as controls. Immunohistochemistry for tyrosine hydroxylase (a marker of catecholaminergic neurons in the locus coeruleus) revealed that APP/PS1 mice had 23% fewer cells in the locus coeruleus compared with aged wild-type mice. APP/PS1 mice also had increased numbers of cell bodies of neurons positive for tyrosine hydroxylase, but fewer tyrosine hydroxylase-positive fibers, which were also short, thick and broken. Quantitative analysis using unbiased stereology showed a significant age-related increase in the mean volume of tyrosine hy- droxylase-positive neurons in aged APP/PS1 mice compared with young APP/PS1 mice. Moreover, the mean volume of tyrosine hydroxylase-positive neurons was positively correlated with the total volume of the locus coeruleus. These findings indicate that noradrenergic neurons and fibers in the locus coeruleus are predisposed to degenerative alterations in APP/PS1 double transgenic mice.
基金supported by NS073857 to OS5T32GM008620 to EGgenerous donations from Cure Medical,Research for Cure,and individual donors
文摘The limited ability for injured adult axons to regenerate is a major cause for limited functional recovery after injury to the nervous system,motivating numerous efforts to uncover mechanisms capable of enhancing regeneration potential.One promising strategy involves deletion or knockdown of the phosphatase and tensin(PTEN) gene.Conditional genetic deletion of PTEN before,immediately following,or several months after spinal cord injury enables neurons of the corticospinal tract(CST) to regenerate their axons across the lesion,which is accompanied by enhanced recovery of skilled voluntary motor functions mediated by the CST.Although conditional genetic deletion or knockdown of PTEN in neurons enables axon regeneration,PTEN is a well-known tumor suppressor and mutations of the PTEN gene disrupt brain development leading to neurological abnormalities including macrocephaly,seizures,and early mortality.The long-term consequences of manipulating PTEN in the adult nervous system,as would be done for therapeutic intervention after injury,are only now being explored.Here,we summarize evidence indicating that long-term deletion of PTEN in mature neurons does not cause evident pathology; indeed,cortical neurons that have lived without PTEN for over 1 year appear robust and healthy.Studies to date provide only a first look at potential negative consequences of PTEN deletion or knockdown,but the absence of any detectable neuropathology supports guarded optimism that interventions to enable axon regeneration after injury are achievable.
文摘Studies on a variety of highly regenerative tissues, including the central nervous system(CNS) in non-mammalian vertebrates, have consistently demonstrated that tissue damage induces the formation of an ionic current at the site of injury. These injury currents generate electric fields(EF) that are 100-fold increased in intensity over that measured for uninjured tissue. In vitro and in vivo experiments have convincingly demonstrated that these electric fields(by their orientation, intensity and duration) can drive the migration, proliferation and differentiation of a host of cell types. These cellular behaviors are all necessary to facilitate regeneration as blocking these EFs at the site of injury inhibits tissue repair while enhancing their intensity promotes repair. Consequently, injury-induced currents, and the EFs they produce, represent a potent and crucial signal to drive tissue regeneration and repair. In this review, we will discuss how injury currents are generated, how cells detect these currents and what cellular responses they can induce. Additionally, we will describe the growing evidence suggesting that EFs play a key role in regulating the cellular response to injury and may be a therapeutic target for inducing regeneration in the mammalian CNS.
基金supported by National Natural Science Foundation of China(No:81500377)the Joint Special Fund between Yunnan Provincial Science and Technology Department and Kunming Medical University(No:2015FB009,2015FB153)Program for Students Innovation in Kunming Medical University
文摘Objective: To investigate the distribution and contents of vimentin(Vim) and glial fibrillary acidic protein(GFAP) immunoreactivities in the central nervous system(CNS)of normal newborn, adult and aged rats.Methods: In this study, thirty healthy and normal Sprague–Dawley rats were simply classified into three groups: Newborn(7 days aged), adult(5 months aged) and aged(24 months aged) rats. Brains and spinal cord were dissected and cut into frozen sections. The expression of Vim and GFAP in CNS were detected by confocal immunofluorescence.Results: In each group, Vim was expressed in all the regions of CNS including the hippocampal, cerebral cortex, the third ventricle and spinal cord, and the expression was highest in neuron-like cell of newborn rats, while Vim was mainly expressed in cell bodies in adult and aged rats. GFAP was expressed in all the regions of CNS including the hippocampal, cerebral cortex, the third ventricle and spinal cord, and the expression was in astrocytes of aged rats. In the third ventricle, Vim was detected in all groups, and only observed in neuron-like cells of newborn. Meanwhile, the GFAP expression showed no significant differences between adult and aged rats in this region. The co-localization of Vim and GFAP were mainly observed in hippocampus and cerebral cortex of newborn,but this co-localization was found in the third ventricle of the rats in all groups.Conclusion: Our data demonstrate for the first time that the expression of Vim and GFAP in the rat's CNS during development. This data may provide a foundation for the further mechanistic studies of these two main intermediate filaments during development of CNS.
基金the National Natural Science Foundation of China, No. 30100098, 30570979
文摘BACKGROUND: Exogenous brain-derived neurotrophic factor (BDNF) promotes retinal ganglion cell survival. However, the protective mechanisms remain unclear. OBJECTIVE: To investigate changes in retinal tyrosine kinase receptor B (trkB) expression and effects of exogenous BDNF on trkB activation in a rat model of acute high intraocular pressure (HtOP). DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Department of Anatomy and Neurobiology, Xiangya Medical School, Central South University from January 2004 to August 2006. MATERIALS: Rabbit anti-BDNF and anti-trkB.FL(full-length) polyclonal antibodies were purchased from Santa Cruz Biotechnology, USA; rabbit anti-p-trkB polyclonal antibodies were purchased from Cellsignal, USA. METHODS: A total of 48 healthy, adult, Sprague Dawiey rats were randomly assigned to acute HIOP (without BDNF pre-treatment) and BDNF pre-treated groups, with 24 animals in each group. In the BDNF pre-treated group, the left eyes were intravitreally injected with 3 pg/kg BDNF 2 days prior to HIOP. Rats in the acute HIOP group were not pre-treated with BDNE HIOP models were established by increased intraocular pressure in the left eyes until the b-wave of flash electroretinogragh disappeared and pressure was maintained for 60 minutes. The right eyes of all rats were not treated and served as the normal controls. MAIN OUTCOME MEASURES: Retinal structure and cell numbers in the ganglion cell layer (GCL) were detected by Nissl staining; expression of trkB and phosphorylated trkB in the rat retina were determined by immunohistochemistry. RESULTS: A greater number of GCL neurons were observed in the pre-treated group compared to the acute HIOP group (P 〈 0.05). TrkB expression was significantly increased following HIOP at days 1 and 3 (P 〈 0.05), but expression varied between retinal areas. Although trkB expression decreased at 7 days, phosphorylated trkB dramatically decreased with increasing time (P 〈 0.05). TrkB expression in BDNF pre-treated rats was similar to the acute HIOP group at early injury time points. Nevertheless, trkB expression was significantly decreased compared to the acute HIOP group at 7 days (P 〈 0.05), and phosphorylated trkB expression was significantly greater compared to the acute HIOP group at each time point (P〈 0.05). CONCLUSION: TrkB expression displayed temporal and spatial changes in the rat retina following acute HIOP, and trkB up-regulation suggested that more BDNF was required for treating the injured retina. Exogenous BDNF partially ameliorated decreased expression of phosphorylated trkB and provided protection to the injured retina, to a certain degree, following HIOP.