Characterization of Nanocomposite Membrane Based Bacterial Cellulose Made of Pineapple Waste Reinforced by Graphite Nanoplatelets

Waste is the main problem for the environment.Handling waste for various useful applications has a benefit for the future.This work has been studied for handling pineapple peel waste to make composite film bacterial cellulose nanocomposite membrane(BCNM)with addition graphite nanoplatelet(GNP).The concentration of GNP in the membrane influence the membrane properties.The bacterial cellulose(BC)pellicle was synthesized by using media from pineapple peel waste extract.BC pellicle is cleaned with water and NaOH solution to be free from impactors.BCNM is synthesized through the mechanical disintegration stage.The results of disintegration using high pressure homogenizer at 150 bar and five cycles.BCNM/GNP is synthesized with varying addition of GNP of 2.5,5.0,10 and 100 wt%of dry bacterial nanocellulose(BNC).The BC and GNP solution were dried in an oven for 14 h at 80℃.BCNM morphology was observed using SEM.GNP is dispersed and distributed in the BC matrix as reinforcement.FTIR analysis shows many peaks of BNC less pronounced with increasing of GNP.The higher concentration of GNP,the rougher of BCNM.The optimum tensile strength of BCNM was achieved after addition GNP of 2.5 wt%.

Variation in the life-history traits of a Schilbid catfish,Clupisoma garua (Hamilton, 1822) in the coastal waters of southern Bangladesh

For the first time, the present study reports the life-history traits, comprising length-frequency distribution （LFD）, sex ratio （SR）, length-weight relationships （LWRs）, condition factors （CFs）, and relative growth （WR）, of Clupisoma garua in the coastal waters of Bangladesh. A total of 150 specimens ranging from 8.60 to 25.20 cm total length （TL） and 4.26 to 128.80 g body weight （BW） were collected using traditional fishing gear from August 2013 to July 2014. The overall sex ratio of males to females in the study did not differ significantly from the expected value of 1：1 （χ2=0.96, P〈0.05） but there were significant sex differences （P〈0.05） in the intercepts and slopes of graphs characterizing traits in C. garua. The calculated b values for the LWRs were 2.955, 2.893 and 2.927 for males, females and combined sexes, respectively, and there was negative allometric growth in all cases （b〈3）. The condition factors （KA, KF, KrR） and relative growth （WR） also did not differ significantly （P〈0.05） between the sexes. This study provides a useful tool for fishery specialists to evaluate the relative condition of fish and to initiate early management strategies and regulations for the sustainable management of the remaining stocks of this species in the entire coastal region of southern Bangladesh.

Preliminary Study on the Distribution of Irrawaddy Dolphin, <i>Orcaella brevirostris</i>, in Banten Bay

Irrawaddy dolphins (Orcaella brevirostris) are aquatic mammals with critically endangered status able to live in different water salinities habitats, like estuary, rivers and lakes. Banten Bay features estuarine area where rapid economic development, contributed from industry, ports, mining and fisheries, takes place, and thus the presence of Irrawaddy dolphins in the area faces various environmental threats. This research aimed to study the distribution of Irrawaddy dolphins in Banten Bay and explore environmental factors threatening its life. Dolphins observation was conducted from January 2013-February 2014, using direct visual effort by team of observer and indirect effort by local fishermen. Direct visual effort was performed on boat by three observers, with maximum vessel speed 15 km/hour and total length of transect was 404 Km. Indirect visual effort comprised of regular assessment by 32 local fishermen when they conducted capture fishing at two different areas (South and North of Banten Bay). Sightings data from direct and indirect visual observation compiled with respondents opinion were mapped using Arc View&reg?GIS 3.3, resulting in distribution map of Irrawaddy dolphin in Banten Bay. Irrawaddy dolphin was most frequently observed in the northeast, south and central areas of the bay. Environmental threats alarming the lives of Irrawaddy dolphin in the Banten Bay may include noise pollution, boat accident and entanglement.

Relationship between the tropical seagrass bed characteristics and the structure of the associated fish community

Structural complexity of seagrass bed including species composition and shoot density is argued to be an important factor determining fish assemblages. However statistical verification of such a relationship is possible only in areas with high species richness of seagrass and fish assemblages which is observed in tropical waters. Material for this study was collected in three seagrass beds with different structure in Inner Ambon Bay, Eastern Indonesia. This study provided evidence that higher structural complexity of seagrass bed was related to the higher richness, abundance, and biomass of fish. However, lower structural complexity of seagrass patch should not be underestimated because it provided different habitat for various stages of life in fish. Smaller fish preferred to occupy dense seagrass of dominant pioneer small-sized species (Halodule uninervis) and moved to the lesser dense bed of climax large-sized seagrass (Thalassia hemprichii and Enhalus acoroides) with increasing their size. This finding is important for seagrass-fisheries management.

Applied Technology to Produce Chlorella Jelly Genki in Order to Increase Human Body Immunity from Covid-19 Illness

The Covid-19 was first confirmed in Indonesia on 2 March 2020.The pandemic has spread to 34 provinces in country with Jakarta,West Java,East Java and Riau being the worst hit.So far,Indonesia has 3,693,272,616 cases,the 6th highest in world behind America,India,Brasil,and Turkey.So it is very important to increase the human body immunity by absorbing CO_(2),haze,dust,tiny particle,and Covid-19,and producing O_(2)in the surrounding area and Chlorella powder as supplement food to prevent fatality of the human being.The Dahril Bottle is a place to culture Chlorella sp.in a closed room to absorb CO_(2),haze,dust,tiny particle,COVID-19,and produce O_(2)in the surrounding area and Chlorella powder that can be used as supplement food to increase the immunity of human body from COVID-19 illness.Based on the result of this study it is indicated that Chlorella with chlorophyll-a inside can grow well in the Dahril Bottle to absorb CO_(2)and produce O_(2)with a chemical reaction as follow,6CO_(2)+6H_(2)O→C_(6)H_(12)O_(6)+O_(2)↑.The high concentration of cell density was found 1.2×10^(7)cells/mL,chlorophyll-a concentration of 184.09μg/L,and Chlorella powder,1.25 g/L,and CO_(2)decreased from 18.36 mg/L to 1.24 mg/L or 93.34%,O_(2)increased from 3.76 mg/L to 6.21 mg/L or 65.15%.The chemical compound of Chlorella powder was also high with a protein level of 45.09%,lipid 10.85%,and carbohydrate 12.77%.They also contain:vitamin E,mineral,beta-carotene,and antioxidant essence.Chlorella Jelly Genki that is made in this study can be used as a supplement food to increase the immunity of the human body to prevent Covid-19 illness.

Biofloc system supplemented by Pseudoalteromonas piscicida 1Ub protects the Pacific white shrimp Penaeus vannamei from Vibrio parahaemolyticus infection

This study aimed to evaluate the supplementation of the probiotic Pseudoalteromonas piscicida 1Ub to the biofloc system as an ecofriendly strategy for protecting white shrimp(Penaeus vannamei)from Vibrio parahaemolyticus infection.Shrimp with an average body weight of(0.50±0.09)g were reared in 30 glass jars with a working volume of 2.5 L at a density of 20 ind/L.Shrimp were reared for 5 d for each treatment,which included the biofloc system without and with 106 colony forming unit(CFU)per mL probiotic.The regular clear water system was used as control.All treatment groups were challenged with 103,105,and 107 CFU/mL V.parahaemolyticus.For the negative control,shrimp were reared without V.parahaemolyticus.The results showed that the density of V.parahaemolyticus cocultured with P.piscicida 1Ub decreased and the density of V.parahaemolyticus in rearing water and shrimp body in the probiotic-treated group was lower than that in the control group(P<0.05).The survival and immune response(total hemocyte count,phagocytic activity,respiratory burst,phenoloxidase,and superoxide dismutase)of shrimp in the probiotic group was higher than that in the positive control(P<0.05).Moreover,supplementing the biofloc system with the probiotic could protect shrimp hepatopancreas from damage caused by V.parahaemolyticus,regardless of bacterial density.Thus,the supplementation of the probiotic P.piscicida 1Ub in the biofloc system could significantly protect and increase the resistance of shrimp to V.parahaemolyticus infection.

Contamination of microplastics in Brantas River, East Java, Indonesia and its distribution in gills and digestive tracts of fish Gambusia affinis

The Brantas River is currently vulnerable to microplastics pollution.Microplastics not only pollute the aquatic environment but also enter the body of fish and other aquatic organisms.This research is aimed at deciding if microplastics were present in the waters and the gills and digestive tract of the Gambusia affinis fish of the river.It also looked at differences in the abundance of several types of microplastics found in the various organ samples and locations.Field research was conducted from January 2020 to March 2020.The microplastics were identified by type,size,color,and the abundance of each type was calculated.The types of microplastics identified were fragments,fibres,films,and pellets.Microplastics of 0.1 mm size are predominant and formed about 76%-100%of the microplastics that were found.Black microplastics were more common in water samples(24%),gills(43%),and digestive tract(46%).The greatest abundance of microplastic fragments was found in water samples of 4066.67 particles/m^(3),1352.78 particles/gram in gill samples,and 2138.89 particles/gram in the digestive tract.Multivariate tests for variants of microplastic types found in the organs at different sampling locations gave a p-value<0.05.These results indicate a difference in the abundance values of microplastic species in different organ samples and sites.

Immunomolecular response of CD4^(+),CD8^(+),TNF-α and IFN-γ in Myxobolus-infected koi(Cyprinus carpio)treated with probiotics

A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,local anti-parasitic drugs(kutuklin),and the chemical compound diflubenzuron treatments on the koi immune response.This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic.The treatments were divided into 5 groups:Treatment(A)(healthy koi without treatment),(B)(infected koi without treatment),(C)(infected koi with 0.55 mL/30 L probiotics),(D)(infected koi with 1μL/g of feed kutuklin),and(E)(infected koi with 0.02 mg/5 L dimilin).Myxospore has observed with Scanning Electron Microscopy(SEM)and 4′,6-diamidino-2-phenylindole(DAPI)fluorescence staining.The histological analysis using semi-quantitative scoring methods,and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4(CD4^(+)),Cluster of differentiation 8(CD8^(+)),Tumor Necrosis Factor-alpha(TNF-α),Interferon gamma(IFN-γ)cells in the gills.Results show that the histological analysis indicated edema,hyperplasia,lamella fusion,congestion,and hypertrophy lesions in infected koi.Treatment with probiotics shows the lowest damage(30.6%).The immune responses of CD4^(+)and CD8^(+)cells to dimilin treatment were 10.54%and 16.86%,respectively.The largest TNF-αand IFN-γresponse were for the kutuklin treatment(29.26%)and probiotics treatment(8.23%).

CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish(Gambusia affinis Baird and Girard 1853)juveniles

This experiment aimed to determine how Cd exposure impacts CD4 cell activation,macrophage cells,pinocytosis activity,metallothionine expression,and Cd levels in juvenile Gambusia affinis gills.Four treatment groups were used,with one control and treatments A,B,C,and D at dosages of 0.03 mg/L,0.023 mg/L,0.015 mg/L,and 0.008 mg/L,respectively.The results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control(0.33%).The value in A,namely 0.54%,was the highest,followed by B,C,and D of 0.46%,0.44%,and 0.42%,respectively.The number of macrophages increased as the Cd level of the medium increased,as did the activity of pinocytosis.Furthermore,the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the gills.Similarly,the MT intensity increased at Cd-exposed gills as compared to the control.As the Cd level in the gills increased,the MT levels ascended significantly.

A multidisciplinary method to assess the reproductive biology of Mystus bleekeri

The study aims to afford a deeper knowledge on reproductive biology of Mystus bleekeri from Surma River,Bangladesh in a multidisciplinary way.Though,the species is in demand as food and ornamental fish in Bangladesh but still now comprehensive details on reproductive biology of this species is scanty.Total 600 fish samples were derived from commercial catches from July 2018 to June 2019.Periodic differences in sex ratio,size distribution,condition factor(Kn),fecundity,gonadosomatic index and gonadal maturation cycle were assessed.The results showed 384(64%)female and 216(36%)male with an overall sex ratio of 1.77:1(female:male).Mean total length(TL)and body weight(BW)of all fish studied were 14.85±3.38 cm TL and 27.54±15.76 g,respectively.The Kn varied with length groups,the highest(1.45±0.23)and lowest(0.89±0.18)values obtained in 16.0-16.9 and 10.0-10.9 cm TL groups,respectively.The highest Kn was documented in October while the lowest was in January.Monthly GSI values showed two peaks in July and November for both the sexes.The fecundity was found to vary from 1120 to 14790 eggs with average value of 4968±3047 with 14.4-18.3 cm in TL and 19.97-34.7g in BW.The relationship of fecundity with TL and BW was F=1.385TL13.405,r^(2)=0.885 and F=5.201BW^(4.827),r^(2)=0.944.Partial behavior of spawning together with allochronic oocytes development has also been found through histology.The results of this study could be used for predicting the response of populations of fish in Surma River and/or other where to human interferences and environmental change.