Objective:To determine the molecular characterization of Polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relalionsliip of Iranian H9N2 viruses and other Asian viruses.Methods:T...Objective:To determine the molecular characterization of Polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relalionsliip of Iranian H9N2 viruses and other Asian viruses.Methods:The Polymerase complex(PA,PBl and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified(by RT-PCR method) and sequenced.Nucleotide sequences(Open Reading Frame: orf) of the PA,PBl and PB2 genes were used for phylogenetic tree construction.Results:Most PB2 and PA genes of the H9N2 viruses isolated in 2008-2009 belonged to the unknown avian sublineage which grouped with the 2004 Pakistani H7N3 viruses.The PBl genes of Iranian viruses indicated greater genetic diversity and shared a high level of similarity to PBl genes from either HS or H7 subtypes with compared to established H9N2 Eurasian sublineages.Condusions:Our findings demonstrated that the H9N2 viruses in Iran exhibit striking reassortment which has led to the generation of new genotypes.展开更多
Herpesviruses account for most of the known virus-encoded miRNAs. Herpesvirus of turkey (HVT), a non-pathogenic avian herpesvirus used as an avian vaccine and viral vector, encodes 28 mature miRNAs. This included HVT-...Herpesviruses account for most of the known virus-encoded miRNAs. Herpesvirus of turkey (HVT), a non-pathogenic avian herpesvirus used as an avian vaccine and viral vector, encodes 28 mature miRNAs. This included HVT-miR-H14-3p that showed almost identical sequence to gga-miR-221, suggesting that it is pirated from the avian host. Although the functional homolog between the two miRNAs has been proposed based on the sequence similarity, the direct experimental evidence is still lacking. In this report, we provide the evidence for the first time that HVT-miR-H14-3p is indeed a gga-miR-221 homolog through modulating the expression of p27Kip1, a known target of miR-221 by binding to its 3’UTR. We also created an HVT-miR-H14-3p deletion virus and show that this miRNA is not essential for in vitro replication.展开更多
基金Supported by The Islamic Azad University,Shoushtar Branch(grantNo.8254)
文摘Objective:To determine the molecular characterization of Polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relalionsliip of Iranian H9N2 viruses and other Asian viruses.Methods:The Polymerase complex(PA,PBl and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified(by RT-PCR method) and sequenced.Nucleotide sequences(Open Reading Frame: orf) of the PA,PBl and PB2 genes were used for phylogenetic tree construction.Results:Most PB2 and PA genes of the H9N2 viruses isolated in 2008-2009 belonged to the unknown avian sublineage which grouped with the 2004 Pakistani H7N3 viruses.The PBl genes of Iranian viruses indicated greater genetic diversity and shared a high level of similarity to PBl genes from either HS or H7 subtypes with compared to established H9N2 Eurasian sublineages.Condusions:Our findings demonstrated that the H9N2 viruses in Iran exhibit striking reassortment which has led to the generation of new genotypes.
文摘Herpesviruses account for most of the known virus-encoded miRNAs. Herpesvirus of turkey (HVT), a non-pathogenic avian herpesvirus used as an avian vaccine and viral vector, encodes 28 mature miRNAs. This included HVT-miR-H14-3p that showed almost identical sequence to gga-miR-221, suggesting that it is pirated from the avian host. Although the functional homolog between the two miRNAs has been proposed based on the sequence similarity, the direct experimental evidence is still lacking. In this report, we provide the evidence for the first time that HVT-miR-H14-3p is indeed a gga-miR-221 homolog through modulating the expression of p27Kip1, a known target of miR-221 by binding to its 3’UTR. We also created an HVT-miR-H14-3p deletion virus and show that this miRNA is not essential for in vitro replication.
