We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in ...We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been ob- served for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass substrate changed from 1.02×10?8 ??1 to 1.41×10?11 ??1 at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)2. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21 polyclonal antibodies interacting with p21 antigen. The pro- posed concept can represent a new immunodiagnostic technique and may have wide ranging applications in biosensors and nanobiotechnology too.展开更多
The aim of this work is to analyze the role of the impressed sources in determining the well or ill-posedness of time harmonic electromagnetic boundary value problems involving isotropic effective media.It is shown,in...The aim of this work is to analyze the role of the impressed sources in determining the well or ill-posedness of time harmonic electromagnetic boundary value problems involving isotropic effective media.It is shown,in particular,that,even if all interfaces are regular,the class of ill-posed problems can be very large in the presence of general square-integrable impressed sources.However,when a simple and realistic constraint is enforced on these sources,requiring that the support of the sources does not include any interface between a traditional medium and a metamaterial,among the problems here considered just those involving an interface between complementary materials remain ill-posed.These considerations have a very significant impact also on the approximability of the solution of well-posed problems since the numerical noise can introduce small fictitious sources even where the sources to be simulated are not present.These effects on finite element simulators are fully analyzed.Finally,we propose an algorithm that allows to obtain much better approximations of the solutions of the most critical wellposed problems.展开更多
文摘We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been ob- served for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass substrate changed from 1.02×10?8 ??1 to 1.41×10?11 ??1 at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)2. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21 polyclonal antibodies interacting with p21 antigen. The pro- posed concept can represent a new immunodiagnostic technique and may have wide ranging applications in biosensors and nanobiotechnology too.
文摘The aim of this work is to analyze the role of the impressed sources in determining the well or ill-posedness of time harmonic electromagnetic boundary value problems involving isotropic effective media.It is shown,in particular,that,even if all interfaces are regular,the class of ill-posed problems can be very large in the presence of general square-integrable impressed sources.However,when a simple and realistic constraint is enforced on these sources,requiring that the support of the sources does not include any interface between a traditional medium and a metamaterial,among the problems here considered just those involving an interface between complementary materials remain ill-posed.These considerations have a very significant impact also on the approximability of the solution of well-posed problems since the numerical noise can introduce small fictitious sources even where the sources to be simulated are not present.These effects on finite element simulators are fully analyzed.Finally,we propose an algorithm that allows to obtain much better approximations of the solutions of the most critical wellposed problems.
