AIM:To explore the effects of CO-releasing molecules tricarbonyldichlororuthenium(Ⅱ)dimer,CORM-2-liberated CO on attenuation of inflammatory responses in liver of an experimental animal model of thermal injury and to...AIM:To explore the effects of CO-releasing molecules tricarbonyldichlororuthenium(Ⅱ)dimer,CORM-2-liberated CO on attenuation of inflammatory responses in liver of an experimental animal model of thermal injury and to investigate the associated potential mechanisms.METHODS:Thirty-six mice were assigned to three groups in three respective experiments.In each experiment,mice in sham group(n=4)received sham thermal injury,whereas mice in burn group(n=4) received a 15%of total body surface area(TBSA)full-thickness thermal injury,and mice in burn+CORM-2 group(n=4)received the same thermal injury with immediate administration of CORM-2(8 mg/kg,iv).Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light icroscope.Levels of aminotransferases(ALT and AST)and nitric oxide(NO) were measured by biochemical methods.Tumor necrosis factor-α(TNF-α)and interleukin(IL-1β)activity,and the protein expression of iNOS and HO-1 in serum and tissue homogenates were assessed.In in vitro experiments,Kupffer cells were stimulated with LPS(10μg/mL)for 4 h in the presence or absence of CORM-2(10-100μmol/L).Subsequently,the expression levels of TNF-αand NO production were assessed.RESULTS:Pro-inflammatory mediators(TNF-α,IL-1β,NO)in serum and liver homogenates of thermally injured mice were significantly reduced by CORM-2 administration.This was accompanied by a decrease in the expression of iNOS while an increase in the expression of HO-1 in the liver tissue.In parallel,the concentrations of TNF-αand NO in supernatants of LPS-stimulated Kupffer cells co-incubated with CORM-2 (10-100μmol/L)were also markedly decreased.Histological examination demonstrated that CORM-2 could attenuate the leukocytes infiltration to the liver tissue. CONCLUSION:CORM-released CO modulates liver inflammation and significantly protects liver injury in burn mice by inhibiting the expression of iNOS and NO production,down-regulating the expression of pro-inflammatory mediators(TNF-α,IL-1β).展开更多
Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native tissues.The introduction of gradient cues usually needs special equipment and complex process but i...Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native tissues.The introduction of gradient cues usually needs special equipment and complex process but is only effective to limited biomaterials.Incorporation of multiple gradients in the hydrogels remains challenges.Here,betasheet rich silk nanofibers(BSNF)were used as building blocks to introduce multiple gradients into different hydrogel systems through the joint action of crosslinking and electric field.The blocks migrated to the anode along the electric field and gradually stagnated due to the solution-hydrogel transition of the systems,finally achieving gradient distribution of the blocks in the formed hydrogels.The gradient distribution of the blocks could be tuned easily through changing different factors such as solution viscosity,which resulted in highly tunable gradient of mechanical cues.The blocks were also aligned under the electric field,endowing orientation gradient simultaneously.Different cargos could be loaded on the blocks and form gradient cues through the same crosslinking-electric field strategy.The building blocks could be introduced to various hydrogels such as Gelatin and NIPAM,indicating the universality.Complex niches with multiple gradient cues could be achieved through the strategy.Silk-based hydrogels with suitable mechanical gradients were fabricated to control the osteogenesis and chondrogenesis.Chondrogenic-osteogenic gradient transition was obtained,which stimulated the ectopic osteochondral tissue regeneration in vivo.The versatility and highly controllability of the strategy as well as multifunction of the building blocks reveal the applicability in complex tissue engineering and various interfacial tissues.展开更多
Varicella is a self-limited disease, but sometimes it may be associated with some serious life-threatening complications. Necrotizing fasciitis is a rare complication of varicella. This is a case of a 7-year-old girl ...Varicella is a self-limited disease, but sometimes it may be associated with some serious life-threatening complications. Necrotizing fasciitis is a rare complication of varicella. This is a case of a 7-year-old girl with septic shock caused by necrotizing fasciitis as a complication of varicella. Swelling and pain in the left inguinal region and left axillary region were found five days after varicella. Then a high fever occurred followed by hypotension. Fluid infusion, vasopressor and antibiotics were administered, Group A beta-hemolytic Streptococcus was isolated from exudates from the wounds. The clinical symptoms markedly improved after surgical drainage and removal of the necrotic tissue. Both wounds were covered with skin grafts after healthy granulation tissue formed. Although there have been few reports of life-threatening necrotizing fasciitis following varicella in western countries, it is rare in China. Usually patients with varicella were admitted to pediatric or infectious disease department but not surgical departments; so that the clinicians should be aware that varicella may be complicated by life-threatening surgical infections. Necrotizing fasciitis should be suspected in patients of varicella who showed an increasing pain and swelling in any body areas associated with increasing fever and local erythema. Early identification, surgical drainage and debridement are essential for successful treatment of necrotizing fasciitis.展开更多
基金National Natural Science Foundation of China, No. 30772256
文摘AIM:To explore the effects of CO-releasing molecules tricarbonyldichlororuthenium(Ⅱ)dimer,CORM-2-liberated CO on attenuation of inflammatory responses in liver of an experimental animal model of thermal injury and to investigate the associated potential mechanisms.METHODS:Thirty-six mice were assigned to three groups in three respective experiments.In each experiment,mice in sham group(n=4)received sham thermal injury,whereas mice in burn group(n=4) received a 15%of total body surface area(TBSA)full-thickness thermal injury,and mice in burn+CORM-2 group(n=4)received the same thermal injury with immediate administration of CORM-2(8 mg/kg,iv).Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light icroscope.Levels of aminotransferases(ALT and AST)and nitric oxide(NO) were measured by biochemical methods.Tumor necrosis factor-α(TNF-α)and interleukin(IL-1β)activity,and the protein expression of iNOS and HO-1 in serum and tissue homogenates were assessed.In in vitro experiments,Kupffer cells were stimulated with LPS(10μg/mL)for 4 h in the presence or absence of CORM-2(10-100μmol/L).Subsequently,the expression levels of TNF-αand NO production were assessed.RESULTS:Pro-inflammatory mediators(TNF-α,IL-1β,NO)in serum and liver homogenates of thermally injured mice were significantly reduced by CORM-2 administration.This was accompanied by a decrease in the expression of iNOS while an increase in the expression of HO-1 in the liver tissue.In parallel,the concentrations of TNF-αand NO in supernatants of LPS-stimulated Kupffer cells co-incubated with CORM-2 (10-100μmol/L)were also markedly decreased.Histological examination demonstrated that CORM-2 could attenuate the leukocytes infiltration to the liver tissue. CONCLUSION:CORM-released CO modulates liver inflammation and significantly protects liver injury in burn mice by inhibiting the expression of iNOS and NO production,down-regulating the expression of pro-inflammatory mediators(TNF-α,IL-1β).
基金We thank the National Key R&D Program of China(2016YFE0204400)National Nature Science Foundation of China(Grant Nos.81171712 and 81873995)We also thank the Social Development Program of Jiangsu Province(BE2018626,BE2019662)for support of this work.
文摘Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native tissues.The introduction of gradient cues usually needs special equipment and complex process but is only effective to limited biomaterials.Incorporation of multiple gradients in the hydrogels remains challenges.Here,betasheet rich silk nanofibers(BSNF)were used as building blocks to introduce multiple gradients into different hydrogel systems through the joint action of crosslinking and electric field.The blocks migrated to the anode along the electric field and gradually stagnated due to the solution-hydrogel transition of the systems,finally achieving gradient distribution of the blocks in the formed hydrogels.The gradient distribution of the blocks could be tuned easily through changing different factors such as solution viscosity,which resulted in highly tunable gradient of mechanical cues.The blocks were also aligned under the electric field,endowing orientation gradient simultaneously.Different cargos could be loaded on the blocks and form gradient cues through the same crosslinking-electric field strategy.The building blocks could be introduced to various hydrogels such as Gelatin and NIPAM,indicating the universality.Complex niches with multiple gradient cues could be achieved through the strategy.Silk-based hydrogels with suitable mechanical gradients were fabricated to control the osteogenesis and chondrogenesis.Chondrogenic-osteogenic gradient transition was obtained,which stimulated the ectopic osteochondral tissue regeneration in vivo.The versatility and highly controllability of the strategy as well as multifunction of the building blocks reveal the applicability in complex tissue engineering and various interfacial tissues.
文摘Varicella is a self-limited disease, but sometimes it may be associated with some serious life-threatening complications. Necrotizing fasciitis is a rare complication of varicella. This is a case of a 7-year-old girl with septic shock caused by necrotizing fasciitis as a complication of varicella. Swelling and pain in the left inguinal region and left axillary region were found five days after varicella. Then a high fever occurred followed by hypotension. Fluid infusion, vasopressor and antibiotics were administered, Group A beta-hemolytic Streptococcus was isolated from exudates from the wounds. The clinical symptoms markedly improved after surgical drainage and removal of the necrotic tissue. Both wounds were covered with skin grafts after healthy granulation tissue formed. Although there have been few reports of life-threatening necrotizing fasciitis following varicella in western countries, it is rare in China. Usually patients with varicella were admitted to pediatric or infectious disease department but not surgical departments; so that the clinicians should be aware that varicella may be complicated by life-threatening surgical infections. Necrotizing fasciitis should be suspected in patients of varicella who showed an increasing pain and swelling in any body areas associated with increasing fever and local erythema. Early identification, surgical drainage and debridement are essential for successful treatment of necrotizing fasciitis.
