The continued development of clustered regularly interspaced short palindromic repeats(CRISPR)technology has the potential to greatly impact clinical medicine,particularly for disease diagnosis and treatment.Despite h...The continued development of clustered regularly interspaced short palindromic repeats(CRISPR)technology has the potential to greatly impact clinical medicine,particularly for disease diagnosis and treatment.Despite high demand for the in vivo delivery of CRISPR-based therapies,significant challenges persist.These include rapid degradation by enzymes,inefficient disease site targeting,and the risk of undesired off-target outcomes.Nanoparticulate platforms,with their tailorable properties,have been engineered to efficiently package CRISPR payloads in various formats,including as plasmid DNA,mRNA,and ribonucleoprotein complexes,for in vivo delivery.Among them,recombinant adeno-associated viruses,virus-like particles,and lipid nanoparticles have displayed exceptional promise.This review will discuss the development of these and other nanocarriers for in vivo CRISPR-based genome editing.展开更多
Perovskite solar cells(PSCs)with a positive-intrinsicnegative(p–i–n,commonly referred to as“inverted”)structure are becoming commercially attractive due to their superior power conversion efficiency(PCE)and better...Perovskite solar cells(PSCs)with a positive-intrinsicnegative(p–i–n,commonly referred to as“inverted”)structure are becoming commercially attractive due to their superior power conversion efficiency(PCE)and better operational stability as compared to the“normal”structure device.^([1–5])Nonetheless,further improvements in the device performance of inverted PSCs are hampered by interface losses,particularly where the buried interfaces are responsible for perovskite crystallization and chargecarrier extraction.^([6–9])展开更多
The growing demand for electric vehicles highlights the need for energy storage solutions with higher densities,spotlighting Li metal anodes as potential successors to traditional Li-ion batteries(LIBs).Achieving long...The growing demand for electric vehicles highlights the need for energy storage solutions with higher densities,spotlighting Li metal anodes as potential successors to traditional Li-ion batteries(LIBs).Achieving longer calendar aging life for Li metal anodes is crucial for their practical use,given their propensity for corrosion due to a low redox potential,which leads to compromised cycling stability and significant capacity loss during storage.Recent research investigated that this susceptibility is mainly dependent on the surface area of Li metal anode and the properties of the solid electrolyte interphase(SEI),particularly its stability and growth rate.Our research adds to this understanding by demonstrating that the amount of Li plating is a key factor in its corrosion during open-circuit storage,as assessed across various electrolytes.We discovered that increasing the Li plating amount effectively reduces Coulombic efficiency(C.E.)loss during aging,due to a lower surface area-to-Li ratio.This implies that the choice of electrolyte for optimal storage life should consider the amount of Li plating,with higher capacities promoting better storage characteristics.展开更多
基金supported by the Defense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense(No.HDTRA1-21-1-0010)the National Institutes of Health(Nos.R21AI159492,and R21AI175904).
文摘The continued development of clustered regularly interspaced short palindromic repeats(CRISPR)technology has the potential to greatly impact clinical medicine,particularly for disease diagnosis and treatment.Despite high demand for the in vivo delivery of CRISPR-based therapies,significant challenges persist.These include rapid degradation by enzymes,inefficient disease site targeting,and the risk of undesired off-target outcomes.Nanoparticulate platforms,with their tailorable properties,have been engineered to efficiently package CRISPR payloads in various formats,including as plasmid DNA,mRNA,and ribonucleoprotein complexes,for in vivo delivery.Among them,recombinant adeno-associated viruses,virus-like particles,and lipid nanoparticles have displayed exceptional promise.This review will discuss the development of these and other nanocarriers for in vivo CRISPR-based genome editing.
文摘Perovskite solar cells(PSCs)with a positive-intrinsicnegative(p–i–n,commonly referred to as“inverted”)structure are becoming commercially attractive due to their superior power conversion efficiency(PCE)and better operational stability as compared to the“normal”structure device.^([1–5])Nonetheless,further improvements in the device performance of inverted PSCs are hampered by interface losses,particularly where the buried interfaces are responsible for perovskite crystallization and chargecarrier extraction.^([6–9])
基金supported by Intelligence Advanced Research Projects Activity under Robust Energy Sources for Intelligence Logistics In Extreme,Novel and Challenging Environments(RESILIENCE)program.
文摘The growing demand for electric vehicles highlights the need for energy storage solutions with higher densities,spotlighting Li metal anodes as potential successors to traditional Li-ion batteries(LIBs).Achieving longer calendar aging life for Li metal anodes is crucial for their practical use,given their propensity for corrosion due to a low redox potential,which leads to compromised cycling stability and significant capacity loss during storage.Recent research investigated that this susceptibility is mainly dependent on the surface area of Li metal anode and the properties of the solid electrolyte interphase(SEI),particularly its stability and growth rate.Our research adds to this understanding by demonstrating that the amount of Li plating is a key factor in its corrosion during open-circuit storage,as assessed across various electrolytes.We discovered that increasing the Li plating amount effectively reduces Coulombic efficiency(C.E.)loss during aging,due to a lower surface area-to-Li ratio.This implies that the choice of electrolyte for optimal storage life should consider the amount of Li plating,with higher capacities promoting better storage characteristics.
