Aim: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. Methods: Children (n = 92) hospitalized with symptoms and signs ...Aim: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. Methods: Children (n = 92) hospitalized with symptoms and signs of acute infections were included and categorized into five groups, i.e. bacterial infection, suspected bacterial infection, viral infection, suspected viral infection and others. Blood was taken at admittance and the following 3-4 d for the measurement of CRP and HNL . Results: Both CRP and HNL were significantly raised at admittance in bacterial infection as compared to viral infection (p < 0.001). After 25-48 h, 83%of the children with bacterial infections still had raised CRP levels in contrast to 11%having raised HNL levels. The levels of CRP, but not those of HNL, were significantly correlated to days of symptoms before admission. Conclusions: HNL is a promising diagnostic tool in the distinction of acute infections caused by bacteria or virus. The differences in the kinetics of CRP and HNL make HNL a bettermarker for monitoring antibacterial treatment, since HNL is probably elevated only when an active bacterial infection is at hand.展开更多
Serum amyloid P component(SAP)is present in seminal plasma,on spermatozoa,and in different tissues of the male reproductive tract,but its function is not known.The aims of this study were to determine if the concentra...Serum amyloid P component(SAP)is present in seminal plasma,on spermatozoa,and in different tissues of the male reproductive tract,but its function is not known.The aims of this study were to determine if the concentration of SAP in seminal plasma is associated with commonly assessed semen parameters and to investigate if SAP could be a new,indirect biomarker for these parameters.In a cross-sectional study of 203 young volunteers,the concentration of SAP in seminal plasma was measured with a in-house developed enzyme-linked immunosorbent assay.Scatter plots,Pearson’s correlation coefficients(r),and linear regression models were produced,and SAP showed a statistically significant correlation with sperm concentration(r=0.75),sperm number(r=0.68),semen volume(r=-0.19),progressive sperm motility(r=0.24),and sperm immotility(r=-0.20).When the study group was dichotomized,SAP could be used to discriminate samples with a sperm concentration<or≥5×10^(6)ml^(-1),15×10^(6)ml^(-1),or 40×10^(6)ml^(-1),and in receiver operating characteristic curves,the corresponding areas under the curves were 0.97,0.93,and 0.82,respectively,with P<0.001 for all three cutoff values studied.The concentration of SAP in seminal plasma showed a strong,positive correlation with the concentration of spermatozoa in semen.SAP may be used as a new indirect potential biomarker for sperm concentration in fresh and in frozen,stored samples.In addition,it is envisaged that the assay could be developed into a home fertility test to differentiate between a low and a normal sperm concentration.展开更多
文摘Aim: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. Methods: Children (n = 92) hospitalized with symptoms and signs of acute infections were included and categorized into five groups, i.e. bacterial infection, suspected bacterial infection, viral infection, suspected viral infection and others. Blood was taken at admittance and the following 3-4 d for the measurement of CRP and HNL . Results: Both CRP and HNL were significantly raised at admittance in bacterial infection as compared to viral infection (p < 0.001). After 25-48 h, 83%of the children with bacterial infections still had raised CRP levels in contrast to 11%having raised HNL levels. The levels of CRP, but not those of HNL, were significantly correlated to days of symptoms before admission. Conclusions: HNL is a promising diagnostic tool in the distinction of acute infections caused by bacteria or virus. The differences in the kinetics of CRP and HNL make HNL a bettermarker for monitoring antibacterial treatment, since HNL is probably elevated only when an active bacterial infection is at hand.
文摘Serum amyloid P component(SAP)is present in seminal plasma,on spermatozoa,and in different tissues of the male reproductive tract,but its function is not known.The aims of this study were to determine if the concentration of SAP in seminal plasma is associated with commonly assessed semen parameters and to investigate if SAP could be a new,indirect biomarker for these parameters.In a cross-sectional study of 203 young volunteers,the concentration of SAP in seminal plasma was measured with a in-house developed enzyme-linked immunosorbent assay.Scatter plots,Pearson’s correlation coefficients(r),and linear regression models were produced,and SAP showed a statistically significant correlation with sperm concentration(r=0.75),sperm number(r=0.68),semen volume(r=-0.19),progressive sperm motility(r=0.24),and sperm immotility(r=-0.20).When the study group was dichotomized,SAP could be used to discriminate samples with a sperm concentration<or≥5×10^(6)ml^(-1),15×10^(6)ml^(-1),or 40×10^(6)ml^(-1),and in receiver operating characteristic curves,the corresponding areas under the curves were 0.97,0.93,and 0.82,respectively,with P<0.001 for all three cutoff values studied.The concentration of SAP in seminal plasma showed a strong,positive correlation with the concentration of spermatozoa in semen.SAP may be used as a new indirect potential biomarker for sperm concentration in fresh and in frozen,stored samples.In addition,it is envisaged that the assay could be developed into a home fertility test to differentiate between a low and a normal sperm concentration.
