Ultrastructure of oval cells in children with chronic hepatitis B,with special emphasis on the stage of liver fibrosis:The first pediatric study

AIM:To investigate the ultrastructure of oval cells in children with chronic hepatitis B,with special emphasis on their location in areas of collagen fibroplasia. METHODS:Morphological investigations were conducted on biopsy material obtained from 40 children,aged 3-16 years with chronic hepatitis B. The stage of fibrosis was assessed histologically using the arbitrary semiquantitative numerical scoring system proposed by Ishak et al. The material for ultrastructural investigation was fixed in glutaraldehyde and paraformaldehyde and processed for transmission-electron microscopic analysis. RESULTS:Ultrastructural examination of biopsy specimens obtained from children with chronic hepatitis B showed the presence of two types of oval cells,the hepatic progenitor cells and intermediate hepatic-like cells. These cells were present in the parenchyma and were seen most commonly in areas of intense periportal fibrosis (at least stage 2 according to Ishak et al) and in the vicinity of the limiting plate of the lobule. The activated nonparenchymal hepatic cells,i.e. transformed hepatic stellate cells and Kupffer cells were seen in close proximity to the intermediate hepatic-like cells. CONCLUSION:We found a distinct relationship between the prevalence of oval cells (hepatic progenitor cells and intermediate hepatocyte-like cells) and fibrosis stage in pediatric patients with chronic hepatitis B.

Ultrastructure of Kupffer cells and hepatocytes in the Dubin-Johnson syndrome:A case report

在肝 bioptate 的 Kupffer 房间和 hepatocytes 的超微结构与 Dubin-Johnson 症候群(DJS ) 在一个 17 岁的男孩被评估。针活体检视获得的肝织物在 glutaraldehyde 和帕拉甲醛被修理并且习惯性地为电子处理了显微镜的分析。肝 bioptate 的极端结构的考试揭示了膜界限的累积，在 hepatocytes 的细胞质以内的电子稠密的 lysosomal 小粒， DJS 特征。他们主要位于胆汁的杆的附近，并且优先地，在小叶中心区域，那对应于在轻显微镜下面看见的颜料存款。小粒的存在被无粒内质网和帕拉的扩大元素伴随水晶的 mitochondrial 包括以及胆汁 canaliculi 的膨胀。在 hepatocytes 的变化与显著刺激共存了并且提高了 Kupffer 房间的吞噬细胞的活动。这在他们对应于在 hepatocytes 观察的那些的细胞质以内在颜料存款的累积被表明。涉及对从瓦解的 hepatocytes 发源的颜料的材料的反应的中央 Kupffer 房间可以玩的活动过度的仙子在 DJS 的发展的一个必要角色。

Matrix-derived serum markers in monitoring liver fibrosis in children with chronic hepatitis B treated with interferon alpha

瞄准：为了有希望地评估， 4 选择了浆液纤维变性标记(tenascin， hyaluronan，骨胶原 VI， TIMP-1 ) 以前，在期间并且在有长期的肝炎 B 的孩子的 IFN 治疗以后的 12 瞬间。方法：有长期的肝炎 B 的 47 个连续病人(范围 4-16 年，平均数 8 年) 经历了 IFN 治疗(为 20 wk 的 3 亩 tiw ) 。纤维变性舞台和发炎等级以前以一种使失明的方式被估计并且在治疗的结束以后的 12 瞬间。浆液纤维变性标记用自动化试金被决定。结果：IFN 治疗改进了组织学的发炎但是没在整个组或在亚群改变纤维变性。仅仅 hyaluronan 与组织学的纤维变性显著地相关(r = 0.3383， P = 0.021 ) 。基础纤维变性标记没在应答者(42.5%) 之间并且非不同应答者(57.5%) 。在 IFN 治疗期间，仅仅浆液 tenascin 在整个组并且在显著地减少了非应答者。预告的处理价值什么时候与相比，在治疗以后珍视 12 瞬间， TIMP-1 在所有病人并且在增加了非，应答者，和 hyaluronan 在所有病人并且在应答者减少了。结论：Tenascin 反映与长期的肝炎 B 在孩子的 IFN 治疗期间减少的肝的纤维发生和发炎。TIMP-1 相互关联与非有组织学的纤维变性的反应和 hyaluronan。