This study aimed at investigating the chitinase enzyme activity produced by chitinolytic bacteria from the skin of blue swimmer crab(Portunus pelagicus)and identification of the genus isolate.This study consists of tw...This study aimed at investigating the chitinase enzyme activity produced by chitinolytic bacteria from the skin of blue swimmer crab(Portunus pelagicus)and identification of the genus isolate.This study consists of two stages:firstly,the qualitative and quantitative activity of the chitinase enzyme;and secondly,biochemical identification of the bacteria.The quantitative chitinase enzyme activity is measured using the UV-Vis spectrophotometer UV-Vis at the wavelength at 660 nm.The chitinase enzyme is obtained from the isolation of chitinolytic bacteria cultured within a media to grow solid chitin,which contains colloidal chitin substrate as chitinase inductor at the temperature of 30℃.The highest chitinolytic activity is obtained from the 24 h supernatant culture,with a value of enzyme activity at 0.149 U/mL.Macroscopic and microscopic identification showed that the chitinolytic bacteria isolate R1,whereas the biochemical cell shows the characteristics of the genus Pseudomonas.展开更多
Fish culture plays an important role in supplying high-quality proteins and omega-3(n-3)long-chain polyunsaturated fatty acids.Aquaculture has been the fastest growing sector in agriculture.Fishmeal is an important co...Fish culture plays an important role in supplying high-quality proteins and omega-3(n-3)long-chain polyunsaturated fatty acids.Aquaculture has been the fastest growing sector in agriculture.Fishmeal is an important component of commercial fish feeds,but ensuring a sustainable supply of fishmeal is in question.A good source of proteins in aquafeeds is insects.For the past 20 years,investigations have been carried out on insect meals as alternative sources for fishmeal.Thus far,the results on replacing fishmeal in feeding aquaculture species have been promising.However,some challenging issues including costs and scaling up of insect production remains.In this review,we aim to summarize the status of applying meals of eight insect species in formulated aquafeeds.We also discussed issues in replacing fishmeal in fish feeds with insect meals and listed some future research directions to make insect meals an important source of proteins for green,profitable and sustainable(GPS)aquaculture.It is certain that in the near future,large-scale insect farming and processing to produce insect meals as an ingredient of fish feeds will have positive impact on the sustainability and profitability of aquaculture.展开更多
文摘This study aimed at investigating the chitinase enzyme activity produced by chitinolytic bacteria from the skin of blue swimmer crab(Portunus pelagicus)and identification of the genus isolate.This study consists of two stages:firstly,the qualitative and quantitative activity of the chitinase enzyme;and secondly,biochemical identification of the bacteria.The quantitative chitinase enzyme activity is measured using the UV-Vis spectrophotometer UV-Vis at the wavelength at 660 nm.The chitinase enzyme is obtained from the isolation of chitinolytic bacteria cultured within a media to grow solid chitin,which contains colloidal chitin substrate as chitinase inductor at the temperature of 30℃.The highest chitinolytic activity is obtained from the 24 h supernatant culture,with a value of enzyme activity at 0.149 U/mL.Macroscopic and microscopic identification showed that the chitinolytic bacteria isolate R1,whereas the biochemical cell shows the characteristics of the genus Pseudomonas.
文摘Fish culture plays an important role in supplying high-quality proteins and omega-3(n-3)long-chain polyunsaturated fatty acids.Aquaculture has been the fastest growing sector in agriculture.Fishmeal is an important component of commercial fish feeds,but ensuring a sustainable supply of fishmeal is in question.A good source of proteins in aquafeeds is insects.For the past 20 years,investigations have been carried out on insect meals as alternative sources for fishmeal.Thus far,the results on replacing fishmeal in feeding aquaculture species have been promising.However,some challenging issues including costs and scaling up of insect production remains.In this review,we aim to summarize the status of applying meals of eight insect species in formulated aquafeeds.We also discussed issues in replacing fishmeal in fish feeds with insect meals and listed some future research directions to make insect meals an important source of proteins for green,profitable and sustainable(GPS)aquaculture.It is certain that in the near future,large-scale insect farming and processing to produce insect meals as an ingredient of fish feeds will have positive impact on the sustainability and profitability of aquaculture.
