Dynamic changes of immune function and inflammatory factors in infants with mycoplasma pneumoniae infection in different stages

Objective:To investigate the levels of immune function and inflammatory factors in children with mycoplasma pneumonia (MP) infection in different stages and its clinical significance. Methods: A total of 100 children with MPP hospitalized from September 2014 to May 2015 were selected as the observation group and divided into two groups of MPP acute period and recovery period on the basis of the infection degree, and 50 healthy children were selected as control group at the same time, detect the immunoglobulin (IgG, IgA, IgM) and complement (C3, C4), peripheral T lymphocyte subsets (CD3+, CD4+, CD8+) and inflammatory factors (IL-2, IL-6, TNF-α, IL-8, IL-10, IL-13) of three groups of infants.Results:immunoglobulin IgG levels of recovery period were significantly higher than that in acute stage and the control group, IgA levels of acute phase and recovery phase were significantly lower than the control group, IgM and complement C3 levels in acute stage were significantly higher than the control group, during the recovery period tended to be normal, complement C4 in acute stage levels were significantly higher than those in recovery period and both higher than the control group. The CD3+, CD4+ and CD4/CD8 ratio of MPP in acute stage and recovery phase were significantly lower than the control group, and these numerical indexes of the recovery period were significantly higher than the acute stage, CD8+ levels in acute stage were significantly higher than that in recovery period and both higher than control group. Compared with the control group ,The levels of inflammatory cytokines IL-2 in the acute phase and recovery phase were significantly decreased, and the recovery period was significantly higher than that in acute stage, the test results that the levels of IL-6, TNF-α, IL-8, IL-10 and IL-13 in acute phase and recovery phase were significantly higher than those in control group and the recovery period was significantly lower than that in the acute stage.Conclusion:The body humoral immune function and cellular immune function damage caused by MP infection in infants and young children could lead to the disorder of immune system and the abnormal expression of inflammatory cytokines. Therefore, Therefore, the real-time monitoring the dynamic level of the indicators can define the infants and young children's condition, improve the recovery rate, and have a certain guiding significance of the clinical diagnosis of infants and young children .

Human Papillomavirus 16E6 Oncogene Mutation in Cervical Cancer

Objective： Cervical cancer （CC） is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses （HR-HPVs） are considered to be the major causes of cervical cancer. HPV16 is the most common type of HR-HPVs and HPV16 E6 gene is one of the major oncogenes. Specific mutations are considered as dangerous factors causing CC. This study was designed to find mutations of HPV16 E6 and the relationship between the mutations and the happening of CC. Methods： The tissue DNA was extracted from 15 biopsies of CC. Part of HPV16 E6 gene （nucleotide 201-523） was amplified by polymerase chain reaction （PCR） from the CC tissue DNA. The PCR fragments were sequenced and analyzed. Results： The result of PCR showed that the positive rate of HPV16 E6 was 93.33% （14/13）. After sequencing ana analyzing, in the 13 out of 14 PCR fragments, 4 maintained prototype （30.77%）, 8 had a same 350G mutation （61.54%）, and 1 had a 249G mutation （7.69%）. Conclusion： This study suggest that there is a high infection rate of HPV in cervical cancer and most of the HPV16 E6 gene has mutations. Those mutations may have an association with the development of cervical cancer.