Wild pistachio tree （Pistacia mutica） is considered the most important species in the Zagros forests. It can adapt to unfavorable environmental conditions. To find the suitable ecological conditions for pistachio sp...Wild pistachio tree （Pistacia mutica） is considered the most important species in the Zagros forests. It can adapt to unfavorable environmental conditions. To find the suitable ecological conditions for pistachio species, we investigated different environmental factors such as gradient, aspect and position of the slopes through the distribution area. Frequency of pistachio trees in the north and northwest of Qalajeh forests was 36.4% and 1%, respectively. Maximum （32%） and minimum （13%） number of wild pistachio trees were in 30%--60% and 〉120% slope classes, respectively. The most number and least number of pistachio trees were found at elevations of 1100-1200 m and 2200-2300 m, respectively. The percentage of pistachio canopy coverage was 10. The average percentage of herbaceous coverage was 6.5. Pistachio trees of diameter per hectare at breast height （DBH） 〉20 cm numbered 5 while average number of pistachio trees was 8 per hectare. Pistachio seedlings per hectare averaged 3.5. The number of other species per ha was 7 trees. The effect of aspect on other species was not significant （p=0.151）. Slope class and geographical aspect did not affect regeneration （p=0.275 and p=0.111, respectively）. Pistachio plays an important economic role in semi-arid areas, therefore it is essential to protect and restore Qalajeh forests through participation by government and local people.展开更多
Supplementation of substrate at casing to increase the yield and quality of mushroom [Agaricus bisporus (Lange) Sing] is an important practice in commercial production of white button mushroom. This project was done t...Supplementation of substrate at casing to increase the yield and quality of mushroom [Agaricus bisporus (Lange) Sing] is an important practice in commercial production of white button mushroom. This project was done to study the effects of supplementing the compost at casing with ground corn and soybean seed applied at: 0 gas control, 17, 34 and51 gper17 kgcompost on production and harvest quality of A. bisporus. There were significant differences between supplemented and non-supplemented substrates. The34 gsoybean and51 gcorn treatments had the highest yield. There were significant differences in quality indices of mushroom due to the type and amount of the supplement. The51 gsoybean supplement produced higher protein compared with other substrates. The highest vitamin C, total phenol, total soluble solids and antioxidant capacity obtained of 34 and51 gsoybeans or 34 and51 gcorn, respectively. Increased production and quality of A. bisporus may be achieved by addition of suitable amounts of supplements.展开更多
Laboratory and greenhouse studies were conducted to assess the allelopathic potential of 12 rice cultivars on barnyard grass. Polyethylene glycol （PEG） was used to determine the influence of osmotic potential on the...Laboratory and greenhouse studies were conducted to assess the allelopathic potential of 12 rice cultivars on barnyard grass. Polyethylene glycol （PEG） was used to determine the influence of osmotic potential on the bioassay materials. Effect of different concentrations of stem, root, and leaf extracts of rice cultivars on seed germination, radicle and primary shoot length of barnyard grass seedlings, and rate of respiration of root pieces were investigated in the laboratory experiments. Shoot height and dry weight of weed stands were studied in the greenhouse, Also total peroxidase activity, chlorophyll pigment and mitotic index were determined. Results indicated that, among rice cultivars, Mehr, Tarom-mahali, G3, Nemat, and Shahpasand caused the most inhibition effects on investigated factors. Amol-3 showed the least negative effects on growth of seedlings and stands of barnyard grass. In laboratory, the Mehr cultivar demonstrated the maximum inhibitory effects by reducing barnyard grass seed germination percentage （88%）, radicle length （100%）, primary shoot length （83%）, and root respiration （85%） Cell division, expressed as mitotic index, was significantly reduced in the presence of rice aqueous extracts. Mehr cultivar had higher inhibitory effect on mitosis compared to Amol-3. In greenhouse, the same cultivar showed the maximum inhibitory effect by reducing barnyard grass height （45%） and dry weight （64%）. With increase in extract concentration, the inhibitory effect increased. Leaf extract from rice plants was more effective compared with the root and stem extracts. Comparison of rice cultivars and PEG indicated that any reduction in germination and growth of barnyard grass using rice extract concentrations of 5% and 10% must have been the result of allelochemicals in the extracts. Lower extract concentrations showed a more pronounced inhibitory effect in the laboratory as compared to the greenhouse studies. These results suggest that rice leaf extracts may be a source of natural herbicide.展开更多
Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of Ficus religiosa in Murashige and Skoog (MS) medium containing differe...Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of Ficus religiosa in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H2O2 (hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for F. religiosa by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules.展开更多
Stenting is a vegetative propagation method, based on cutting and grafting in one action. The question addressed in this study is whether indole-3-butyric acid (IBA) concentration is able to impress success of Chinese...Stenting is a vegetative propagation method, based on cutting and grafting in one action. The question addressed in this study is whether indole-3-butyric acid (IBA) concentration is able to impress success of Chinese hibiscus propagation when grafted onto two kinds of rootstocks. The used IBA concentrations include: 0, 1000, 3000 and 5000 ppm and two cultivars of Hibiscus syriacus (“Blue Stain” and “Jeanne d’Arc”), which have been used as rootstock. Results indicated that, all the cultivars were superior for most of the measured parameters when IBA treatments were used compared to control plants except for average of the longest root size. The highest healing percentage resulted in stentlings (plant which has been propagated by stenting method) with 5000 ppm of IBA treatment. All IBA treatments significantly increased rooting percentage compared with the control plants. Stentlings that were treated with 3000 and 5000 ppm of IBA, produced the greatest leave number. In Chinese hibiscus/“Jeanne d’Arc” combination, higher root number and dry weight of roots were observed. Interactions of kinds of rootstock and IBA concentration showed that the highest healing percentage was obtained in “Blue Stain” in concentration of 5000 ppm and the highest root number were seen in stentlings with “Jeanne d’Arc” in 3000 ppm and “Jeanne d’Arc” in 5000 ppm.展开更多
Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely...Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely unknown. To understand the function of auxin during apple fruit maturation and ripening, auxin efflux carrier and IAA-amido synthetase encoding genes were identified from the apple genome based on the results of previous microarray analysis. The expression patterns of these genes were analyzed using qRT-PCR during 10 - 12 weeks of fruit maturation for two apple cultivars: “Golden Delicious” (GD) and “Cripps Pink” (CP), which have the distinct patterns of maturation progression. Our results showed that the expressions of auxin efflux carrier and IAA-amido synthetase genes have a correlation with the timing of ethylene biosynthesis pathway activation in both cultivars. The earlier and stronger expression of MdGH3.102 and MdAECFP1 in the fruit of GD, a mid-season cultivar, correlates with the earlier activation of a pre-climacteric ethylene biosynthesis gene of MdACS3, compared with that in CP, a late-ripening apple cultivar. Results of exogenous IAA treatment indicated that the expression patterns of the genes were regulated in a fruit maturity dependent manner. Our results suggested that the dynamics of the auxin level in apple fruit cortex could be one of the key factors influencing the timing of ethylene biosynthesis pathway activation and consequently contributed to the control of the apple maturation progression.展开更多
Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hai...Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hairy roots,the activity of antioxidant enzymes compared to normal leaves and roots,and the presence of some phenolic compounds in comparison with fruits were investigated.The IC_(50) values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays.By calculating the infectivity index of peripheral blood mononuclear cells(PBMCs),the leishmanicidal activity(IC_(50) values)of hairy roots for amastigotes was estimated.The effects of hairy roots(IC_(50) values)treatment on the levels of IFN-γ and iNOS expression,intracellular reactive oxygen species,and iNOS protein expression in infected-PBMCs were determined.Results:Based on antioxidant enzyme assays and high performance liquid chromatography analysis,hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds.According to the results of tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays,the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes.Depending on the concentration and exposure time,treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species,up-regulation of IFN-γ and iNOS genes expression,and high value of iNOS protein compared to controls.Conclusions:The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.展开更多
Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%d.b.Increasing of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55...Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%d.b.Increasing of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55 mm),thickness(0.97-1.05 mm),arithmetic mean diameter(2.48-2.73),geometric mean diameter(1.88-2.09 mm),surface area(10.34-12.66 mm^(2)),thousand seed weight(2.9-3.9 g),porosity(51.22%-64.11%),true density(917.8-1030.6 kg/m3),static angle of repose(43-49 deg),dynamic angle of repose(47-56.6 deg),and coefficient of static friction on the three surfaces:glass(0.48-0.77),galvanized iron sheet(0.36-0.73),and plywood(0.57-0.69).However,bulk density was found to decrease from 447.66-369.88 kg/m3,and rupture force and rupture energy along with seed length and width were found to decrease from 83.74-56.17 N,132.95-84.47 N,50.66-27.52 mJ,and 67.8 to 33.36 mJ,respectively.The sphericity increased from 36.63%to 37.5%with increasing in moisture content from 7.24%to 14.5%d.b.and then reduced to 37.5%with further increase in moisture content to 21.38%d.b.展开更多
文摘Wild pistachio tree （Pistacia mutica） is considered the most important species in the Zagros forests. It can adapt to unfavorable environmental conditions. To find the suitable ecological conditions for pistachio species, we investigated different environmental factors such as gradient, aspect and position of the slopes through the distribution area. Frequency of pistachio trees in the north and northwest of Qalajeh forests was 36.4% and 1%, respectively. Maximum （32%） and minimum （13%） number of wild pistachio trees were in 30%--60% and 〉120% slope classes, respectively. The most number and least number of pistachio trees were found at elevations of 1100-1200 m and 2200-2300 m, respectively. The percentage of pistachio canopy coverage was 10. The average percentage of herbaceous coverage was 6.5. Pistachio trees of diameter per hectare at breast height （DBH） 〉20 cm numbered 5 while average number of pistachio trees was 8 per hectare. Pistachio seedlings per hectare averaged 3.5. The number of other species per ha was 7 trees. The effect of aspect on other species was not significant （p=0.151）. Slope class and geographical aspect did not affect regeneration （p=0.275 and p=0.111, respectively）. Pistachio plays an important economic role in semi-arid areas, therefore it is essential to protect and restore Qalajeh forests through participation by government and local people.
文摘Supplementation of substrate at casing to increase the yield and quality of mushroom [Agaricus bisporus (Lange) Sing] is an important practice in commercial production of white button mushroom. This project was done to study the effects of supplementing the compost at casing with ground corn and soybean seed applied at: 0 gas control, 17, 34 and51 gper17 kgcompost on production and harvest quality of A. bisporus. There were significant differences between supplemented and non-supplemented substrates. The34 gsoybean and51 gcorn treatments had the highest yield. There were significant differences in quality indices of mushroom due to the type and amount of the supplement. The51 gsoybean supplement produced higher protein compared with other substrates. The highest vitamin C, total phenol, total soluble solids and antioxidant capacity obtained of 34 and51 gsoybeans or 34 and51 gcorn, respectively. Increased production and quality of A. bisporus may be achieved by addition of suitable amounts of supplements.
文摘Laboratory and greenhouse studies were conducted to assess the allelopathic potential of 12 rice cultivars on barnyard grass. Polyethylene glycol （PEG） was used to determine the influence of osmotic potential on the bioassay materials. Effect of different concentrations of stem, root, and leaf extracts of rice cultivars on seed germination, radicle and primary shoot length of barnyard grass seedlings, and rate of respiration of root pieces were investigated in the laboratory experiments. Shoot height and dry weight of weed stands were studied in the greenhouse, Also total peroxidase activity, chlorophyll pigment and mitotic index were determined. Results indicated that, among rice cultivars, Mehr, Tarom-mahali, G3, Nemat, and Shahpasand caused the most inhibition effects on investigated factors. Amol-3 showed the least negative effects on growth of seedlings and stands of barnyard grass. In laboratory, the Mehr cultivar demonstrated the maximum inhibitory effects by reducing barnyard grass seed germination percentage （88%）, radicle length （100%）, primary shoot length （83%）, and root respiration （85%） Cell division, expressed as mitotic index, was significantly reduced in the presence of rice aqueous extracts. Mehr cultivar had higher inhibitory effect on mitosis compared to Amol-3. In greenhouse, the same cultivar showed the maximum inhibitory effect by reducing barnyard grass height （45%） and dry weight （64%）. With increase in extract concentration, the inhibitory effect increased. Leaf extract from rice plants was more effective compared with the root and stem extracts. Comparison of rice cultivars and PEG indicated that any reduction in germination and growth of barnyard grass using rice extract concentrations of 5% and 10% must have been the result of allelochemicals in the extracts. Lower extract concentrations showed a more pronounced inhibitory effect in the laboratory as compared to the greenhouse studies. These results suggest that rice leaf extracts may be a source of natural herbicide.
文摘Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of Ficus religiosa in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H2O2 (hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for F. religiosa by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules.
文摘Stenting is a vegetative propagation method, based on cutting and grafting in one action. The question addressed in this study is whether indole-3-butyric acid (IBA) concentration is able to impress success of Chinese hibiscus propagation when grafted onto two kinds of rootstocks. The used IBA concentrations include: 0, 1000, 3000 and 5000 ppm and two cultivars of Hibiscus syriacus (“Blue Stain” and “Jeanne d’Arc”), which have been used as rootstock. Results indicated that, all the cultivars were superior for most of the measured parameters when IBA treatments were used compared to control plants except for average of the longest root size. The highest healing percentage resulted in stentlings (plant which has been propagated by stenting method) with 5000 ppm of IBA treatment. All IBA treatments significantly increased rooting percentage compared with the control plants. Stentlings that were treated with 3000 and 5000 ppm of IBA, produced the greatest leave number. In Chinese hibiscus/“Jeanne d’Arc” combination, higher root number and dry weight of roots were observed. Interactions of kinds of rootstock and IBA concentration showed that the highest healing percentage was obtained in “Blue Stain” in concentration of 5000 ppm and the highest root number were seen in stentlings with “Jeanne d’Arc” in 3000 ppm and “Jeanne d’Arc” in 5000 ppm.
文摘Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely unknown. To understand the function of auxin during apple fruit maturation and ripening, auxin efflux carrier and IAA-amido synthetase encoding genes were identified from the apple genome based on the results of previous microarray analysis. The expression patterns of these genes were analyzed using qRT-PCR during 10 - 12 weeks of fruit maturation for two apple cultivars: “Golden Delicious” (GD) and “Cripps Pink” (CP), which have the distinct patterns of maturation progression. Our results showed that the expressions of auxin efflux carrier and IAA-amido synthetase genes have a correlation with the timing of ethylene biosynthesis pathway activation in both cultivars. The earlier and stronger expression of MdGH3.102 and MdAECFP1 in the fruit of GD, a mid-season cultivar, correlates with the earlier activation of a pre-climacteric ethylene biosynthesis gene of MdACS3, compared with that in CP, a late-ripening apple cultivar. Results of exogenous IAA treatment indicated that the expression patterns of the genes were regulated in a fruit maturity dependent manner. Our results suggested that the dynamics of the auxin level in apple fruit cortex could be one of the key factors influencing the timing of ethylene biosynthesis pathway activation and consequently contributed to the control of the apple maturation progression.
文摘Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hairy roots,the activity of antioxidant enzymes compared to normal leaves and roots,and the presence of some phenolic compounds in comparison with fruits were investigated.The IC_(50) values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays.By calculating the infectivity index of peripheral blood mononuclear cells(PBMCs),the leishmanicidal activity(IC_(50) values)of hairy roots for amastigotes was estimated.The effects of hairy roots(IC_(50) values)treatment on the levels of IFN-γ and iNOS expression,intracellular reactive oxygen species,and iNOS protein expression in infected-PBMCs were determined.Results:Based on antioxidant enzyme assays and high performance liquid chromatography analysis,hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds.According to the results of tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays,the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes.Depending on the concentration and exposure time,treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species,up-regulation of IFN-γ and iNOS genes expression,and high value of iNOS protein compared to controls.Conclusions:The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.
文摘Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%d.b.Increasing of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55 mm),thickness(0.97-1.05 mm),arithmetic mean diameter(2.48-2.73),geometric mean diameter(1.88-2.09 mm),surface area(10.34-12.66 mm^(2)),thousand seed weight(2.9-3.9 g),porosity(51.22%-64.11%),true density(917.8-1030.6 kg/m3),static angle of repose(43-49 deg),dynamic angle of repose(47-56.6 deg),and coefficient of static friction on the three surfaces:glass(0.48-0.77),galvanized iron sheet(0.36-0.73),and plywood(0.57-0.69).However,bulk density was found to decrease from 447.66-369.88 kg/m3,and rupture force and rupture energy along with seed length and width were found to decrease from 83.74-56.17 N,132.95-84.47 N,50.66-27.52 mJ,and 67.8 to 33.36 mJ,respectively.The sphericity increased from 36.63%to 37.5%with increasing in moisture content from 7.24%to 14.5%d.b.and then reduced to 37.5%with further increase in moisture content to 21.38%d.b.
