Polycomb chromobox(CBX)proteins regulate gene transcription by maintaining chromatin states,which guide a variety of biological processes.Now,epigenetic regulation of innate immune response is an emerging field.Howeve...Polycomb chromobox(CBX)proteins regulate gene transcription by maintaining chromatin states,which guide a variety of biological processes.Now,epigenetic regulation of innate immune response is an emerging field.However,the role of CBX proteins in innate immunity remains unclear.We confirmed that the expression of CBX family proteins,especially Cbx2,was decreased in macrophages upon viral infection,and then we investigated the role of Cbx2 in the antiviral immune response.Silencing or knockdown of Cbx2 in macrophages inhibited virus-induced production of IFNp.Furthermore,heterozygous Cbx2 knockout were susceptible to VSV challenge.Mechanistically,Cbx2 binds to and recruits Jmjd3 to the Ifnb promoter,leading to demethylation of H3K27me3 and increased transcription of IFN-β.Together,our study reveals a nontraditional function of a Cbx protein and adds new insight into the epigenetic regulation of antiviral innate immunity.展开更多
文摘Polycomb chromobox(CBX)proteins regulate gene transcription by maintaining chromatin states,which guide a variety of biological processes.Now,epigenetic regulation of innate immune response is an emerging field.However,the role of CBX proteins in innate immunity remains unclear.We confirmed that the expression of CBX family proteins,especially Cbx2,was decreased in macrophages upon viral infection,and then we investigated the role of Cbx2 in the antiviral immune response.Silencing or knockdown of Cbx2 in macrophages inhibited virus-induced production of IFNp.Furthermore,heterozygous Cbx2 knockout were susceptible to VSV challenge.Mechanistically,Cbx2 binds to and recruits Jmjd3 to the Ifnb promoter,leading to demethylation of H3K27me3 and increased transcription of IFN-β.Together,our study reveals a nontraditional function of a Cbx protein and adds new insight into the epigenetic regulation of antiviral innate immunity.
