Background Scrub typhus,an acute febrile disease caused by Orientia tsutsugamushi,is transmitted to humans through infected chigger mites.We present a case of scrub typhus in a previously healthy man from Shandong Pro...Background Scrub typhus,an acute febrile disease caused by Orientia tsutsugamushi,is transmitted to humans through infected chigger mites.We present a case of scrub typhus in a previously healthy man from Shandong Province diagnosed using next-generation sequencing(NGS)and PCR and review recent literature on NGS for scrub typhus diagnosis.Methods NGS was utilized for testing whole blood collected on admission.Confirmatory testing was done by detecting IgM and IgG antibodies to Orientia in acute and convalescent sera by ELISA.Orientia 47-kDa protein gene TaqMan and standard PCR of the 56-kDa protein gene and Sanger sequencing were performed on eschar scab DNA.Results The NGS diagnosis was confirmed by 47-kDa protein gene TaqMan and sequencing of a fragment of the O.tsutsugamushi 56-kDa protein gene from the eschar scab.Analysis of this sequence and the NGS data indicated O.tsutsugamushi strain Cheeloo2020 is a novel genotype.Mapping of the NGS data against the O.tsutsugamushi Gilliam strain genome sequence identified 304 reads with high similarity.Conclusions NGS is not only useful for multiplex diagnosis of scrub typhus,but also provides insight into the genetic diversity of O.tsutsugamushi.The common failure to submit sequences to databases makes it difficult to determine the minimal quantity and quality of NGS data being used for the positive identification of Orientia DNA in clinical specimens.展开更多
文摘Background Scrub typhus,an acute febrile disease caused by Orientia tsutsugamushi,is transmitted to humans through infected chigger mites.We present a case of scrub typhus in a previously healthy man from Shandong Province diagnosed using next-generation sequencing(NGS)and PCR and review recent literature on NGS for scrub typhus diagnosis.Methods NGS was utilized for testing whole blood collected on admission.Confirmatory testing was done by detecting IgM and IgG antibodies to Orientia in acute and convalescent sera by ELISA.Orientia 47-kDa protein gene TaqMan and standard PCR of the 56-kDa protein gene and Sanger sequencing were performed on eschar scab DNA.Results The NGS diagnosis was confirmed by 47-kDa protein gene TaqMan and sequencing of a fragment of the O.tsutsugamushi 56-kDa protein gene from the eschar scab.Analysis of this sequence and the NGS data indicated O.tsutsugamushi strain Cheeloo2020 is a novel genotype.Mapping of the NGS data against the O.tsutsugamushi Gilliam strain genome sequence identified 304 reads with high similarity.Conclusions NGS is not only useful for multiplex diagnosis of scrub typhus,but also provides insight into the genetic diversity of O.tsutsugamushi.The common failure to submit sequences to databases makes it difficult to determine the minimal quantity and quality of NGS data being used for the positive identification of Orientia DNA in clinical specimens.
