期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum 被引量:8
1
作者 Fatuma Felix Felician Rui-He Yu +6 位作者 Meng-Zhen Li Chun-Jie Li Hui-Qin Chen Ying Jiang Tao Tang Wei-Yan Qi Han-Mei Xu 《Chinese Journal of Traumatology》 CAS CSCD 2019年第1期12-20,共9页
Purpose: Wound represents a major health challenge as they consume a large amount of healthcare resources to improve patient's quality of life. Many scientific studies have been conducted in search of ideal biomat... Purpose: Wound represents a major health challenge as they consume a large amount of healthcare resources to improve patient's quality of life. Many scientific studies have been conducted in search of ideal biomaterials with wound-healing activity for clinical use and collagen has been proven to be a suitable can didate biomaterial. This study in tended to investigate the wound healing activity of collagen peptides derived from jellyfish following oral administration. Methods: In this study, collagen was extracted from the jellyfish-Rhopilema esculentum using 1% pepsin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fourier transform infrared (FTIR) were used to identify and determine the molecular weight of the jellyfish collagen. Collagenase II, papain and alkaline proteinase were used to breakdown jellyfish collagen into collagen peptides. Wound scratch assay (in vitro) was done to determine migration potential of human umbilical vein endothelial cells (HUVEC) covering the artificial wound created on the cell monolayer following treatment with collagen peptides. In vivo studies were con ducted to determine the effects of collagen peptides on wound healing by examining wound contraction, re-epithelialization, tissue regeneration and collagen deposition on the wounded skin of mice. Confidence level (p < 0.05) was considered significant using GraphPad Prism software. Results: The yield of collagen was 4.31%. The SDS-PAGE and FTIR showed that extracted collagen from jellyfish was type I. Enzymatic hydrolysis of this collagen using collagenase II produced collagen peptides (CPi) and hydrolysis with alkaline proteinase/papain resulted into collagen peptides (CP2). Tricine SDSPAGE revealed that collagen peptides consisted of protein fragments with molecular weight <25 kDa. Wound scratch assay showed that there were significant effects on the scratch closure on cells treated with collagen peptides at a concentration of 6.25 μg/mL for 48 h as compared to the vehicle treated cells. Overall treatment with collagen peptide on mice with full thickness excised wounds had a positive result in wound contraction as compared with the control. Histological assessment of peptides treated mice models showed remarkable sign of re-epithelialization, tissue regeneration and increased collagen deposition. Immunohistochemistry of the skin sections showed a significant increase in β-fibroblast growth factor (β-FGF) and the transforming growth factor-βi (TGF-βi) expression on collagen peptides treated group. Conclusion: Collagen peptides derived from the jellyfish-Rhopilema esculentum can accelerate the wound healing process thus could be a therapeutic potential product that may be beneficial in wound clinics in the future. 展开更多
关键词 COLLAGEN COLLAGEN PEPTIDES Wound HEALING JELLYFISH
原文传递
Optimized functional and structural design of dual-target LMRAP,a bifunctional fusion protein with a 25-amino-acid antitumor peptide and GnRH Fc fragment 被引量:3
2
作者 Meng Li Hanmei Xu Junzhi Wang 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2020年第2期262-275,共14页
To develop fusion protein of a GnRH Fc fragment and the integrin targeting AP25 antitumor peptide for GnRH receptor-expressing cancer therapy.The LMRAP fusion protein was constructed.A transwell invasion assay was per... To develop fusion protein of a GnRH Fc fragment and the integrin targeting AP25 antitumor peptide for GnRH receptor-expressing cancer therapy.The LMRAP fusion protein was constructed.A transwell invasion assay was performed.The gene mRNA and protein levels of GnRHR-I,α5β1,andαvβ3 in different cancer cell lines were assessed.Cell proliferation was measured using a cell counting kit-8.An antagonist assay was performed on GnRH receptors.Anti-tumor activity was evaluated with a mouse xenograft tumor model.Immunohistochemistry(IHC)was applied to detect CD31 and CD34 expressions.Pharmacokinetic characteristics were determined with an indirect competition ELISA.The developed bifunctional fusion protein LMRAP not only inhibited HUVEC invasion,but also inhibited proliferation of GnRHR-I,α5β1,and high expression cancer cells.The IC50 for LMRAP in the GnRH receptor was 6.235 x 10-4 mol/L.LMRAP significantly inhibited human prostate cancer cell line22 RV1 proliferation in vivo and in vitro.LMRAP significantly inhibited CD31 and CD34 expressions.The elimination half-life of the fusion protein LMRAP was 33 h in rats.The fusion protein made of a GnRH Fc fragment and the integrin targeting AP25 peptide retained the bifunctional biological activity of GnRHR blocking,angiogenesis inhibition,prolonged half-life and good tolerance. 展开更多
关键词 Fusion protein GNRH INTEGRIN Angiogenesis Prostate cancer
原文传递
New arbutin derivatives from the leaves of Heliciopsis lobata with cytotoxicity 被引量:1
3
作者 QI Wei-Yan OU Na +1 位作者 WU Xiao-Dong XU Han-Mei 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2016年第10期789-793,共5页
Heliciopsis lobata is a medicinal plant, which is exclusively used to treat tumor in Li folk region. Two new arbutin derivatives, 6'-((E)2-methoxy-5-hydroxycinnamoyl) arbutin(1) and 2'-((E)2, 5-dihydroxycinnam... Heliciopsis lobata is a medicinal plant, which is exclusively used to treat tumor in Li folk region. Two new arbutin derivatives, 6'-((E)2-methoxy-5-hydroxycinnamoyl) arbutin(1) and 2'-((E)2, 5-dihydroxycinnamoyl) arbutin(2) along with five known compounds(3–7), were isolated from the leaves of Heliciopsis lobata. Their structures were elucidated on the basis of extensive spectroscopic interpretations. They were evaluated for their potential anticancer activity. Compounds 6 and 7 exhibited cytotoxicity against MGC-803 cells with IC_(50) values being 44.1 and 11.3 μg·m L^(–1), respectively. Additionally, compounds 1, 2 and 5–7 exhibited a moderate inhibition of MGC-803 cells invasion; compound 2 at 20 μg·m L^(–1) inhibited the invasion of MGC-803 cells by 43.0%, compared with the controls.. 展开更多
关键词 Heliciopsis lobata ARBUTIN Anticancer effects INVASION
原文传递
Integrins as attractive targets for cancer therapeutics 被引量:12
4
作者 Meng Li Ying Wang +3 位作者 Mengwei Li Xuezhen Wu Sarra Setrerrahmane Hanmei Xu 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2021年第9期2726-2737,共12页
Integrins are transmembrane receptors that have been implicated in the biology of various human physiological and pathological processes.These molecules facilitate cell-extracellular matrix and cell-cell interactions,... Integrins are transmembrane receptors that have been implicated in the biology of various human physiological and pathological processes.These molecules facilitate cell-extracellular matrix and cell-cell interactions,and they have been implicated in fibrosis,inflammation,thrombosis,and tumor metastasis.The role of integrins in tumor progression makes them promising targets for cancer treatment,and certain integrin antagonists,such as antibodies and synthetic peptides,have been effectively utilized in the clinic for cancer therapy.Here,we discuss the evidence and knowledge on the contribution of integrins to cancer biology.Furthermore,we summarize the clinical attempts targeting this family in anti-cancer therapy development. 展开更多
关键词 INTEGRINS Extracellular matrix Tumor progression Targeted drug ANTAGONISTS Clinical trial
原文传递
Centranthera grandiflore alleviates alcohol-induced oxidative stress and cell apoptosis 被引量:2
5
作者 GAO Xin-Mei DONG Wen-Han +5 位作者 XIA Chun-Lei MA Zhuo-Yi WANG Ying SARRA Setrerrahmane XU Han-Mei QI Wei-Yan 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2022年第8期572-579,共8页
Alcohol liver disease(ALD)has become a global threat to human health.It is associated with a wide range of liver diseases including alcohol fatty liver,steatosis,fibrosis and cirrhosis,and finally leads to liver cance... Alcohol liver disease(ALD)has become a global threat to human health.It is associated with a wide range of liver diseases including alcohol fatty liver,steatosis,fibrosis and cirrhosis,and finally leads to liver cancer and even death.Centranthera grandiflora is a traditional Chinese medicinal herb commonly used to treat ALD,but no research about its mechanism is available.This study evaluated the hepatoprotective effect and mechanism of C.grandiflora against alcohol-induced liver injury in mice.We found that the ethanol extracts of C.grandiflora(CgW)alleviated the alcohol-induced liver injury,enhanced the levels of antioxidant enzymes,and reduced the amount of lipid peroxides.CgW also affected cell apoptosis by inhibiting the activity of Bax,cleaved-caspase 3 and cleaved-caspase 9,and increasing the activity of Bcl-2.In conclusion,the results showed that CgW can effectively improve ALD through alleviating oxidative stress and inhibiting cell apoptosis for the first time.This study suggested that C.grandiflora is a promising herbal medicine for ALD treatment. 展开更多
关键词 Centranthera grandiflora Alcohol liver diseases Oxidative stress APOPTOSIS Hepatoprotective effect
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部