Reactive oxygen species(ROS)are byproducts of cellular metabolism;they play a significant role as secondary messengers in cell signaling.In cells,high concentrations of ROS induce apoptosis,senescence,and contact inhi...Reactive oxygen species(ROS)are byproducts of cellular metabolism;they play a significant role as secondary messengers in cell signaling.In cells,high concentrations of ROS induce apoptosis,senescence,and contact inhibition,while low concentrations of ROS result in angiogenesis,proliferation,and cytoskeleton remodeling.Thus,controlling ROS generation is an important factor in cell biology.We designed a chlorin e6(Ce6)-immobilized polyethylene terephthalate(PET)film(Ce6-PET)to produce extracellular ROS under red-light irradiation.The application of Ce6-PET films can regulate the generation of ROS by altering the intensity of light-emitting diode sources.We confirmed that the Ce6-PET film could effectively promote cell growth under irradiation at 500 μW/cm^(2) for 30 min in human umbilical vein endothelial cells.We also found that the Ce6-PET film is more efficient in generating ROS than a Ce6-incorporated polyurethane film under the same conditions.Ce6-PET fabrication shows promise for improving the localized delivery of extracellular ROS and regulating ROS formation through the optimization of irradiation intensity.展开更多
基金This work was supported by a National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT,Nos 2017M3A9B3063638 and 2019R1A2C2005256).
文摘Reactive oxygen species(ROS)are byproducts of cellular metabolism;they play a significant role as secondary messengers in cell signaling.In cells,high concentrations of ROS induce apoptosis,senescence,and contact inhibition,while low concentrations of ROS result in angiogenesis,proliferation,and cytoskeleton remodeling.Thus,controlling ROS generation is an important factor in cell biology.We designed a chlorin e6(Ce6)-immobilized polyethylene terephthalate(PET)film(Ce6-PET)to produce extracellular ROS under red-light irradiation.The application of Ce6-PET films can regulate the generation of ROS by altering the intensity of light-emitting diode sources.We confirmed that the Ce6-PET film could effectively promote cell growth under irradiation at 500 μW/cm^(2) for 30 min in human umbilical vein endothelial cells.We also found that the Ce6-PET film is more efficient in generating ROS than a Ce6-incorporated polyurethane film under the same conditions.Ce6-PET fabrication shows promise for improving the localized delivery of extracellular ROS and regulating ROS formation through the optimization of irradiation intensity.
