AIM： To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology. METHODS： Male C57BI_/6J mice underwent common bile duct ligation （BDL） and were matched with p...AIM： To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology. METHODS： Male C57BI_/6J mice underwent common bile duct ligation （BDL） and were matched with pairfed sham-operated controls. After 7 d, the animals were sacrificed and total RNA was isolated from livers and kidneys. Equal amounts of RNA from each tissue were pooled for each group and hybridized to Affymetrix GeneChip^MG-U74Av2 containing a total of 12488 probe sets. Data analysis was performed using GeneSpring 6.0 software. Northern analysis and immunofluorescence were used for validation. RESULTS： In sham-operated and BDL mice, 44 and 50% of 12488 genes were expressed in livers, whereas 49 and 51% were expressed in kidneys, respectively. Seven days after BDL, 265 liver and 112 kidney genes with GeneOntology annotation were up-regulated and 113 liver and 36 kidney genes were down-regulated in comparison with sham-operated controls. Many genes were commonly regulated in both tissues and metabolism-related genes represented the largest functional group. CONCLUSION： Following BDL, microarray analysis reveals a broad range of gene alterations in both liver and kidney.展开更多
基金Supported by the USPHS grants DK 25636 (J. L. B.)the Yale Liver Center Cellular and Molecular Physiology and Morphology Cores (P30-34989)the Deutsche Forschungsgemeinschaft Grant DE 872/1-1 (G. U. D.)
文摘AIM： To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology. METHODS： Male C57BI_/6J mice underwent common bile duct ligation （BDL） and were matched with pairfed sham-operated controls. After 7 d, the animals were sacrificed and total RNA was isolated from livers and kidneys. Equal amounts of RNA from each tissue were pooled for each group and hybridized to Affymetrix GeneChip^MG-U74Av2 containing a total of 12488 probe sets. Data analysis was performed using GeneSpring 6.0 software. Northern analysis and immunofluorescence were used for validation. RESULTS： In sham-operated and BDL mice, 44 and 50% of 12488 genes were expressed in livers, whereas 49 and 51% were expressed in kidneys, respectively. Seven days after BDL, 265 liver and 112 kidney genes with GeneOntology annotation were up-regulated and 113 liver and 36 kidney genes were down-regulated in comparison with sham-operated controls. Many genes were commonly regulated in both tissues and metabolism-related genes represented the largest functional group. CONCLUSION： Following BDL, microarray analysis reveals a broad range of gene alterations in both liver and kidney.
