POM analysis and computational interactions of 8-hydroxydiospyrin inside active site of protein tyrosine phosphatase 1B

Proteintyrosine phosphatase 1B(PTP1B)inhibitionis consideredas a potentialtherapeuticfor the treatmentof cancer,type2 diabetes,andobesity.Inour presentwork,weinvestigatedtheanti-diabeticpotentialof8-hydroxydiospyrin(8-HDN)from D.lotus against the PTP1B enzyme.It showed significant inhibitory activity of PTP1B with an IC 50 value of 18.37±0.02μM.A detailed molecular docking study was carried out to analyze the binding orientation,binding energy,and mechanism of inhibition.A comparative investigation of 8-HDN in the catalytic,as well as the allosteric site of PTP1B,was performed.Binding energy data showed that compound 8-HDN is more selective for the allosteric site and hence avoids the problems associated with catalytic site inhibition.The inhibition mechanism of 8-HDN can be further investigated as an active lead compound against PTP1B by using in vitro and in vivo models.

Link between COVID-19 vaccines and myocardial infarction

BACKGROUND Vaccines for coronavirus disease 2019(COVID-19) include Ch Ad Ox1-SARS-COV-2(Astra Zeneca),Ad26.COV2.S(Janssen),m RNA-1273(Moderna),BNT162b2(Pfizer),BBIBP-Cor V(Sinopharm),Corona Vac(Sinovac),and Bharat Biotech BBV152(Covaxin).AIM To find the association between COVID-19 vaccines and myocardial infarction(MI).METHODS This is a systematic review that involved searching databases such as MEDLINE,EMBASE,and Pak Medi Net after making a search strategy using Me SH and Emtree terms.Eligibility criteria were set,and studies having no mention of MI as a complication of COVID-19 vaccination,protocols,genetic studies,and animal studies were excluded.Data was extracted using a predesigned extraction table,and 29 studies were selected after screening and applying the eligibility criteria.RESULTS The majority of studies mentioned Astra Zeneca(18 studies) followed by Pfizer(14 studies) and Moderna(9 studies) in subjects reporting MI after vaccination.Out of all the studies,69% reported MI cases after the first COVID-19 vaccination dose and 14% after the second,44% reported ST-segment elevation MI,and 26% reported non-ST-segment elevation MI.The mortality rate was 29% after MI.CONCLUSION In conclusion,many studies linked MI to COVID-19 vaccinations,but no definitive association could be found.