Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from...Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from being obtained from deep regions of tissue.We review commontechniques,such as multiphoton microscopy(MPM)and optical coherence microscopy(OCM),for diffraction limited imaging beyond an imaging depth of 0.5 mm.Novel implementations havebeen emerging in recent years giving higher imaging speed,deeper penetration,and better imagequality.Focal modulation microscopy(FMM)is a novel method that combines confocal spatialfltering with focal modulation to reject out-of-focus background.FMM has demonstrated animaging depth comparable to those of MPM and OCM,near-real-time image acquisition,and thecapability for multiple contrast mechanisms.展开更多
In a previous study, structure of silica gels prepared in a high magnetic field was investigated. While a direct application of such anisotropic silica gels is for an optical anisotropic medium possessing chemical res...In a previous study, structure of silica gels prepared in a high magnetic field was investigated. While a direct application of such anisotropic silica gels is for an optical anisotropic medium possessing chemical resistance, we show here their possibility of medium in materials processing. In this direction, for example, silica hydrogels have so far been used as media of crystal growth. In this paper, as opposed to the soft-wet state, dried silica gels have been investigated. We have found that lead （II） nanocrystallites were formed induced by electron irradiation to lead （ll）-doped dried Hydrogels made from a sodium metasilicate solution doped with silica gels prepared in a high magnetic field such as B = 10 T. lead （II） acetate were prepared. The dried specimens were irradiated by electrons in a transmission electron microscope environment. Electron diffraction patterns indicated the crystallinity of lead （II） nanocrystallites depending on B. An advantage of this processing technique is that the crystallinity can be controlled through the strength of magnetic field B applied during gel preparation. Specific skills are not required to control the strength of magnetic field.展开更多
文摘Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from being obtained from deep regions of tissue.We review commontechniques,such as multiphoton microscopy(MPM)and optical coherence microscopy(OCM),for diffraction limited imaging beyond an imaging depth of 0.5 mm.Novel implementations havebeen emerging in recent years giving higher imaging speed,deeper penetration,and better imagequality.Focal modulation microscopy(FMM)is a novel method that combines confocal spatialfltering with focal modulation to reject out-of-focus background.FMM has demonstrated animaging depth comparable to those of MPM and OCM,near-real-time image acquisition,and thecapability for multiple contrast mechanisms.
文摘In a previous study, structure of silica gels prepared in a high magnetic field was investigated. While a direct application of such anisotropic silica gels is for an optical anisotropic medium possessing chemical resistance, we show here their possibility of medium in materials processing. In this direction, for example, silica hydrogels have so far been used as media of crystal growth. In this paper, as opposed to the soft-wet state, dried silica gels have been investigated. We have found that lead （II） nanocrystallites were formed induced by electron irradiation to lead （ll）-doped dried Hydrogels made from a sodium metasilicate solution doped with silica gels prepared in a high magnetic field such as B = 10 T. lead （II） acetate were prepared. The dried specimens were irradiated by electrons in a transmission electron microscope environment. Electron diffraction patterns indicated the crystallinity of lead （II） nanocrystallites depending on B. An advantage of this processing technique is that the crystallinity can be controlled through the strength of magnetic field B applied during gel preparation. Specific skills are not required to control the strength of magnetic field.
