Exploring the medication pattern and mechanism of action of traditional Chinese medicine in treating polycystic ovary syndrome with kidney deficiency and blood stasis based on data mining and network pharmacology

Background:Using network pharmacology to explore the potential molecular mechanism of traditional Chinese medicine in treating polycystic ovary syndrome(PCOS)with kidney deficiency and blood stasis syndrome.Method:Collect the related literature materials of PCOS with kidney deficiency and blood stasis syndrome treated by traditional Chinese medicine in four databases in recent ten years,extract the information of prescriptions and complete the frequency analysis.Traditional Chinese Medicine Systems Pharmacology Database was used to screen out the effective components.Use Online Mendelian Inheritance in Man and other databases to screen PCOS disease targets.The intersection targets obtained by clustering prescription and PCOS disease targets were submitted to STRING database for protein-protein interaction network analysis,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes pathways were analysed by Metascape.Result:There are 155 kinds of traditional Chinese medicines used in the literature.The most commonly utilized ones are Cuscutae Semen,Angelicae Sinensis Radix,and Rehmanniae Radix Praeparata.The results of the cluster analysis indicated that the plants most commonly found throughout the prescription were Leonuri Herba,Lycopi Herba,Dipsaci Radix,etc.GO results show that biological processes include cell reaction to organic nitrogen compounds and cell reaction to nitrogen compounds.The functional display of GO molecule includes cytokine receptor binding,signal receptor regulator activity and so on.Kyoto Encyclopedia of Genes and Genomes results show that the possible mechanisms of action are cancer pathway,an endocrine resistance signal pathway.Conclusion:Through data mining,the cluster prescription for PCOS with kidney deficiency and blood stasis syndrome is Leonuri Herba,Lycopi Herba,Dipsaci Radix,etc.The network pharmacology research of cluster prescription shows that the main drug components for treating PCOS with kidney deficiency and blood stasis syndrome are quercetin,kaempferol,luteolin,tanshinone IIA,etc.,which act on PTGS2,NCOA2,and other targets,and treat PCOS with kidney deficiency and blood stasis syndrome through cancer and endocrine resistance.

Effect of circuit exercise training combined with drug therapy on glucose lipid metabolism and microinflammatory state in T2DM patients

Objective: To study the effect of circuit exercise training combined with drug therapy on glucose lipid metabolism and microinflammatory state in T2DM patients. Methods: Patients with newly diagnosed T2DM who were treated in Dazhou Integrated Traditional Chinese and Western Medicine Hospital between June 2014 and October 2016 were selected and randomly divided into training intervention group and routine intervention group who received circuit exercise training intervention and routine exercise intervention respectively. The contents of glucose lipid metabolism indexes and inflammatory cytokines in serum as well as the contents of inflammatory cells in peripheral blood were determined before intervention and 8 weeks after intervention. Results: Serum FBG, F-Ins, HbA1c%, FFA, TC, LDL-C, ox-LDL, Chemerin, Resistin, IL-6, MCP-1 and CRP contents as well as peripheral blood CD14+CD16+ cell contents of both groups of patients after intervention were lower than those before intervention while HDL-C, ADP and Vaspin contents were higher than those before intervention, and serum FBG, F-Ins, HbA1c%, FFA, TC, LDL-C, ox-LDL, Chemerin, Resistin, IL-6, MCP-1 and CRP contents as well as peripheral blood CD14+CD16+ cell content of training intervention group after intervention were lower than those of routine intervention group while HDL-C, ADP and Vaspin contents were higher than those of routine intervention group. Conclusion: Circuit exercise training for T2DM can be more effective in improving glucose lipid metabolism and inhibiting inflammatory response.

Efficacy of Shenfu decoction on sepsis in rats with condition induced by cecal ligation and puncture

OBJECTIVE:To evaluate the efficacy of Shenfu decoction(SFD)prepared with a traditional Chinese formula,on sepsis in rats with the condition induced by cecal ligation and puncture(CLP),and to study the possible mechanism underlying its action.METHODS:Forty clean-grade male Sprague-Dawley rats were divided randomly into three groups:normal control group(NCG,n=10),model control group(MCG,n=15)and Shenfu decoction group(SFDG,n=15).Sham-operated rats in NCG were served as operation control,while rats in both MCG and SFDG were exposed to CLP,a procedure to develop experimental sepsis.Rats in SFDG were administered with SFD by gavage(3 mg/g of body weight,twice a day)2 h prior to CLP and directly after successful CLP,while rats in NCG and MCG were gavaged with equivalent volume of sterilized water.Rats in all groups were starved with free access to drink.After 24 h of administration,the mortality of rats in each group was assessed.The indicators of inflammatory response[the peritoneal inflammation by Simon's method Classification as well as serum concentrations of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)by enzyme linked immunosorbent assay(ELISA)]in survival rats were evaluated.The indicators of gut barrier[The intestinal mucosal injury index,serum concentrations of D-lactic acid and secretory IgA(sIgA)in intestinal mucosa by ELISA,as well as gut microbiota by16S rRNA gene sequencing]in survival rats were evaluated.RESULTS:The mortality(20%)of rats in SFDG was lower than that(33.3%)of the MCG(P<0.01).The mortality(20%)of rats in SFDG was lower than that(33.3%)of the MCG(χ2=6.533,P=0.011).Compared with the MCG,the peritoneal inflammation as well as serum concentrations of TNF-αand IL-6 decreased significantly in SFDG(all P<0.01).Compared with the MCG,the IMII,serum concentrations of D-lactic acid,sIgA in intestinal mucosa were alleviated by SFD treatment(all P<0.01).Increase in levels of Proteobacteria and reduction levels of Bacteroidetes induced by sepsis were observed,and these two disturbed gut microbiota phyla could be regulated after SFD treatment.Increase in levels of Proteobacteria and reduction levels of Bacteroidetes induced by sepsis were observed,and these two disturbed gut microbiota phyla could be regulated after SFD treatment.CONCLUSION:SFD may play a protective role in sepsis by alleviating sepsis-induced inflammatory response and gut barrier damage in rats.