Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresist...Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresistance in GBM and to find ways to improve radiosensitivity.Here,we found that heat shock protein 90 beta family member 1(HSP90B1)was significantly upregulated in radioresistant GBM cell lines.More importantly,HSP90B1 promoted the localization of glucose transporter type 1,a key rate-limiting factor of glycolysis,on the plasma membrane,which in turn enhanced glycolytic activity and subsequently tumor growth and radioresistance of GBM cells.These findings imply that targeting HSP90B1 may effectively improve the efficacy of radiotherapy for GBM patients,a potential new approach to the treatment of glioblastoma.展开更多
Circulating tumor DNA(ctDNA)is a DNA fragment released into the peripheral blood by apoptotic or necrotic tumor cells,which carry the basic genetic information after gene mutation.The detection and analysis of ctDNA c...Circulating tumor DNA(ctDNA)is a DNA fragment released into the peripheral blood by apoptotic or necrotic tumor cells,which carry the basic genetic information after gene mutation.The detection and analysis of ctDNA can provide genomic information on the size and development of tumor;therefore,it is considered as an emerging and promising tumor biomarker for cancer progression,reoccurrence,and routine monitoring after surgery.Albeit the isolation method is relatively simple due to the stability of ctDNA,the abundance of ctDNA is extremely low,and result from the high content of background cell free DNA(cfDNA),the large difference between individuals,and the need of predicted detection sites,it is necessary to analyze ctDNA in a comprehensive way.At present,the main methods that meet the needs of ctDNA detection with sensitivity and specificity are amplification refractory mutation system PCR(ARMS-PCR),digital-PCR(dPCR)and high-throughput sequencing.Among them,dPCR has advantages in practicality,which can realize absolute quantification of single-molecule DNA,detect and trace DNA molecules and conduct quantitative analysis,providing a reliable basis for clinical monitoring of tumor recurrence and minimal residual diseases.The study reviews the detection and potential value of ctDNA in gynecologic tumors.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.82072765 to X.Q.and 82172667 to X.W.).
文摘Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresistance in GBM and to find ways to improve radiosensitivity.Here,we found that heat shock protein 90 beta family member 1(HSP90B1)was significantly upregulated in radioresistant GBM cell lines.More importantly,HSP90B1 promoted the localization of glucose transporter type 1,a key rate-limiting factor of glycolysis,on the plasma membrane,which in turn enhanced glycolytic activity and subsequently tumor growth and radioresistance of GBM cells.These findings imply that targeting HSP90B1 may effectively improve the efficacy of radiotherapy for GBM patients,a potential new approach to the treatment of glioblastoma.
文摘Circulating tumor DNA(ctDNA)is a DNA fragment released into the peripheral blood by apoptotic or necrotic tumor cells,which carry the basic genetic information after gene mutation.The detection and analysis of ctDNA can provide genomic information on the size and development of tumor;therefore,it is considered as an emerging and promising tumor biomarker for cancer progression,reoccurrence,and routine monitoring after surgery.Albeit the isolation method is relatively simple due to the stability of ctDNA,the abundance of ctDNA is extremely low,and result from the high content of background cell free DNA(cfDNA),the large difference between individuals,and the need of predicted detection sites,it is necessary to analyze ctDNA in a comprehensive way.At present,the main methods that meet the needs of ctDNA detection with sensitivity and specificity are amplification refractory mutation system PCR(ARMS-PCR),digital-PCR(dPCR)and high-throughput sequencing.Among them,dPCR has advantages in practicality,which can realize absolute quantification of single-molecule DNA,detect and trace DNA molecules and conduct quantitative analysis,providing a reliable basis for clinical monitoring of tumor recurrence and minimal residual diseases.The study reviews the detection and potential value of ctDNA in gynecologic tumors.
