The influence of in ovo injection with the prebiotic DiNovoon the development of histomorphological parameters of the duodenum, body mass and productivity in large-scale poultry production conditions

Background: Among various feed additives currently used in poultry nutrition, an important role is played by bioactive substances, including prebiotics. The beneficial effect of these bioactive substances on the gastrointestinal tract and immune system give rise to improvements in broiler health and performance nutrition, thus increasing the productivity of these birds. An innovative method for introducing bioactive substances into chickens is the in ovo injection into eggs intended for hatching. The aim of the study was to evaluate the development of histomorphological parameters of the duodenum and productivity in chickens injected in ovo with the prebiotic DiNovo~?(extract of Laminaria species of seaweed, BioA tlantis Ltd., Ireland) on d 12 of incubation, under large-scale, high density poultry production conditions.Results: There was no significant impact of the injection of DiNovo~? prebiotic on the production parameters of broiler chickens(body weight, FCR, EBI and mortality) obtained on d 42 of rearing. No significant impact of the DiNovo~? injection on the duodenum weight and length was observed, as wel as on the CSA, diameter and muscular layer thickness of the duodenum. The in ovo injection of DiNovo~? significantly increased the width of the duodenal vil i(P < 0.05) and crypt depth(P < 0.01) of chickens on d 21 of rearing. Other histomorphological parameters of duodenal vil i at d 42 of chickens rearing such as: the height, width, and cross section area of vil i were significantly greater in chickens from the control group compared to those from the DiNovo~? group(P < 0.05 and P < 0.01).Conclusions: In conclusion, this study demonstrates that injection of DiNovo~? prebiotic into the air chamber of egg significantly influences the histomorphological parameters on d 21 of rearing without negatively affecting productivity in chickens at the end of rearing.

The impact of synbiotic administration through in ovo technology on the microstructure of a broiler chicken small intestine tissue on the 1^(st) and 42^(nd) day of rearing

Background： Application the innovative method which is in ovo technology provides a means of modulating the immune system at early embryonic stages. The aim of study was to determine influence of the in ovo stimulation, on d12 of incubation, with synbiotics（synbiotic 1-L. salivarius IBB3154 ＋ Bi^2tos, Clasado Ltd. and the synbiotic 2-L. plantarum IBB3036 ＋ lupin RFOs） on the microstructure of duodenum, jejunum and ileum in the 1^（st） and 42^（nd）day of rearing.Results： On the 1^（st） day of chickens life, in the duodenum of both experimental groups（SYN1 and SYN2）, a significantly higher and wider intestinal vil i as wel as a significantly larger absorbent surface of these villi were found in comparison with the Control group（P ≤ 0.01）. On the 42^（nd）day of rearing the beneficial effect of synbiotic 1 was reflected by the numerical y higher vil i（no statistical differences） with a larger surface（P ≤ 0.01） in the duodenum in the SYN1 group compare to the Control group. In the jejunum on the 1^（st） day of life, in the SYN1 group, significantly higher vil i than in the Control group, with a simultaneous decrease in the depth of crypts（P ≤ 0.01）, and also the largest width of vil i and their absorbent area（P ≤ 0.01） in comparison to the other groups were found. On the 42^（nd）day of life, in the jejunum, an increase in the height of the vil i whilst reducing the crypt depth in the SYN2 group was found（P ≤ 0.01）. In turn, in the SYN1 group, there were significantly more neutral goblet cel s observed compared with the control group（P ≤ 0.05）. In the ileum of 1-day-old chickens, the widest vil i（P ≤ 0.05） and the deepest crypts（P ≤ 0.01） were found in the SYN2 group. In the same group, there was also the least amount of neutral goblet cel s in comparison to the other groups（P ≤ 0.05）.Conclusions： We observed that synbiotic 1 and 2 beneficial y affected the examined characteristics on the 1^（st） and 42^（nd） day of life. The obtained results al ow us to conclude that the use of synbiotics significantly affect gut structure which should contribute to improvement in nutrient absorption by the gut.

To Knowledge of the Origin of the Brain Mental Activity

This paper is devoted towards the origin of a human subjective state, and we present a methodology for studying the origin of brain activity. We were first to reveal that the subjective states of a human being can be subjected to an objective remote registration. Recently we have established the existence of the so-called “psychogenic field”, which reflects the subjective state of human brain. The subjective state of a human being was shown to affect remotely the physicochemical properties of the blood. The interaction of neurophysiological and subjective processes is described in the context of systemic organization of a goal-directed behavior. An original schematic diagram is presented to describe the formation of a subjective brain state. This approach is based on the feedback influence of a psychogenic field on neuronal molecular processes (self-induction in the brain). We propose a paradigm for the origin of a subjective state and possible existence of physical phenomena and brain fields, which are unique for the active brain.

Letter to the editor“regarding case report of chronic cornual ectopic pregnancy presenting as large cornual mass after in vitro fertilization-embryo transfer”

We read the Yang et al,published case report in Laparoscopic,Endoscopic and Robotic Surgery.2019.https://doi.org/10.1016/j.lers.2019.09.001,with great interest.Yang et al,in the introduction mentioned that the cornual ectopic pregnancy is defined as a pregnancy that is abnormally located in the proximal portion of the fallopian tube,lying within the muscular wall of the uterus.

Parallel fluorescence and phosphorescence monitoring of singlet oxygen photosensitization in rats

The time-and spectral-resolved set-up for measurements of weak infrared luminescence of photosensitizers(PSs)and singlet oxygen using optical lightguides was used on skin of laboratory animals in vivo.Wistar rats with abdominal incisions treated by methylis aminolevulinitis(MAL)were used as a model.A control group of animals with abdominal incisions was also tested.Spectrally resolved fluorescence of the PS was acquired during the treatment from the same spot.The intensity and spectral profile of the fiuorescence signal from the skin can be used to guide the detection setup to the investigated spots in the lesion.The rate of bleaching of Protoporphyrin IX band and appearance of a band of its photoproducts during the treatment can be characterized by the exposition ED,under which the latter becomes dominant feature infiuorescence spectrum.ED value differs statistically significantly between the normal skin and the lesion treated by MAL.No direct proportionality was found between the fluorescence signal and singlet oxygen production.Nevertheless,the strong fluorescence signal is necessary but not a su±cient condition for higher singlet oxygen production in vivo.ED value correlates rather well with production of singlet oxygen,but differently in lesion and normal skin.Lifetimes of singlet oxygen differ between spots outside and in the lesion.PS triplet state lifetimes exhibit weak di®erence between spots treated and untreated by MAL.

Etiopathogenesis of reproductive tract infections and the emerging role of bitter taste receptors:A scoping review

Reproductive tract infections pose an immense public health concern worldwide as over 600 million new cases are recorded annually along with several complications,including infertility,ectopic pregnancy,preterm delivery,and neonatal death.Despite improved understanding of the mechanisms of pathogenic invasion,the etiopathogenesis of reproductive tract infections is yet to be completely understood.Recent data has suggested a critical role of bitter taste receptors of the reproductive tract in etiopathogenesis of reproductive tract infections.Here,we review the literature on current etiopathogenesis of reproductive tract infections and the role of bitter taste receptors of the reproductive tract in etiopathogenesis of reproductive tract infections.Emerging evidence suggests a critical role of microbiota disorder of the reproductive tract in reproductive tract infections.Several bacterial,protozoan parasitic,helminthic parasitic and viral pathogens have been identified as causative agents of reproductive tract infections.These pathogens subvert host defenses and activate specific architectural units of the uroepithelium such as Toll-like receptors,which recognize conserved motifs on the pathogens.The activated Toll-like receptors mediate downstream signaling,stimulating nuclear factor-κB,which in turn activates the production of proinflammatory cytokines.This pathway is also associated with recruitment of immunocytes to the site of aggression and release of proteinases,which drive tissue damage in the reproductive tract.Defects in detection of pathogenic components by the bitter taste receptors of the reproductive tract may play a critical role in the etiopathogenesis of reproductive tract infections.This review provides important information for identification of novel frontiers for the treatment of reproductive tract infections.

The psychophysics of а human brain subjective activity

Recently we have established the existence of a “psychogenic field”, which are most likely reflecting on the human brain subjective state. The goal of this study is to further explore the human subjective state which can be registered remotely and objectively in the context of human subjective state. The analysis of the remote influence of a human subjective state on the blood physical and chemical parameters appeared to be main driving horse. The concept of specific physical phenomena in the brain does not exist in the abiocoen. Future exploring pattern of the relationships between the neurophysiologic and subjective processes in the systemic organization of “goal-seeking” behavior suggests that the paradigm presuming the existence of physical phenomena as a unique interrelationship for the active human brain, and their role in the origin of a subjective state.

Influence of Carcinogenic Factors in the Maxillofacial Region

Objective To study the influence of carcinogenic factors on tissues and organs of the maxillofacial region(MFR)by measuring the expression of biomarkers in the oral fluid.Methods We recruited 421 patients who were divided into 2 groups:356 patients with a tumour of the MFR and 65 healthy people without somatic pathology in medical history.Results The effects of carcinogenic factors on the expression of biomarkers in the oral fluid in patients with a tumour of the MFR revealed a significant increase in the levels of biomarkers of tobacco use-MMP-8,TIMP-1,TIMP-2,and CA 72-4,CEA and CA 125;frequent use or abuse of alcohol-MMP-8,TIMP-1,TIMP-2,AFP and NSE;use of narcotics-TIMP-1 and TIMP-2;presence of occupational hazards in the medical history-MMP-8,MMP-9,TIMP-1 and TIMP-2;with a diagnostic sensitivity(DS)of over 60%.Also our analysis showed that the use of tobacco was significantly affecting the expression of TIMP-1and CA 125;frequent use or abuse of alcohol-CA 19-9,AFP and CEA;use of drugs had an impact on MMP-8expression.However,the occupational hazards in the medical history did not show a statistically significant effect.Conclusion Factors of carcinogenesis affect the expression of biomarkers in the oral fluid.The expression of the biomarkers of the MFR should be compared with that in the control group of patients with no carcinogenic factors.

Constitutional and Anatomical Characteristics of Mature Women

Objective To identify the constitutional and anatomical peculiarities of constitution of women of mature age.Methods There was completed comprehensive anthropometric and bio-electrical survey of 651mature women(relative norm)living in the Moscow region.Results The quantitative distribution of women by somatotypological affiliation was revealed;anthropometric and body component composition in representatives of different somatotypes were defined.Conclusion Thus,the performed study revealed and quantiely characterised the distribution of women according to their constitutional types in the studied population of mature age women living in Moscow region under the relative norm conditions.

Results and Prospects of Experimental Validation of the Use of Electromagnetic Waves at Terahertz Frequencies of Cellular Metabolites in Clinical Practice

The paper presents the results of experimental studies of the effect of irradiation with electromagnetic waves terahertz frequencies of molecular spectrum of emission and absorption of nitric oxide and atmospheric oxygen on animals in a state of acute and chronic immobilization stress, a condition regarded as a model of violations in different parts of the vascular system including microcirculation specific for different parts of the vascular system, including the microcirculation characteristic of various diseases of the cardiovascular system.

Morphological Characteristics of Thymus in the Newborns in Different Climatic and Geographical Conditions of Kyrgyzstan

This paper is describing a detailed study of morphological structures and characteristics newborns thymus in different climatic and geographical conditions of Kara-Balta, Cholpon-Ata, and Bishkek in Kyrgyzstan. Anatomical structure research done on 26 thymuses of newborn corpses. Research results showed that a significant amount of thymuses consisted of lobes with very thin connective tissues between them. It is observed that the Hassall cells were usually located in the medulla part of the thymus after using the coloring by Van-Gieson’s stain visible clear elastic and collagen fibers. In addition, cell population dynamics in a unit of conditional area of cortical substance thymus lobes in newborns determined.

Somatotypological Features of the Skin Fat Fold Thickness in Ethnic Kyrgyz Women

The purpose of this study was to determine the thickness of skin and fat folds in Kyrgyz women of various ages, taking into account their somatotypological profile. Using the method of complex anthropometry, including the determination of the values of 21 anthropometric parameters, the physical status of 1028 Kyrgyz women of different age groups was studied youth (16 - 20 years old 310 girls), mature age (1st period, 21 - 35 years old 308 women;2nd period, 36 - 55 years 410 women) living in Osh, Kyrgyzstan. For somatotyping, we used the scheme of constitutional diagnostics. Seven somatotypes distinguished within three constitutional groups. The subcutaneous fat was measured by caliperometry. Statistical processing carried out using the statistical programs Microsoft Excel and the STATISTICA package (v. 6.0). To determine the reliability of the differences between the indicators, the Student’s test was used (p < 0.05). The results demonstrate that within each somatotype there are broad changes in the studied anthropometric indicator. Thus, the thickness of the skin and fat folds is the smallest in women of asthenic, athletic and stenoplastic (p < 0.05), and the largest in representatives of the euriplastic and pycnic somatotypes (p < 0.05). Thus, indicators of physical status in adolescence and adulthood in women have a pronounced somatotypological specificity. These materials on the physical development and constitutional and typological characteristics of the studied population of women applicable for a personalized approach in the context of a relative norm.

Do reactive oxygen species damage or protect the heart in ischemia and reperfusion?Analysis on experimental and clinical data

The role of reactive oxygen species(ROS)in ischemic and reperfusion(I/R)injury of the heart has been discussed for more than 40 years.It has been demonstrated that reperfusion triggers a multiple increase in free radical generation in the isolated heart.Antioxidants were found to have the ability to mitigate I/R injury of the heart.However,it is unclear whether their cardioprotective effect truly depends on the decrease of ROS levels in myocardial tissues.Since high doses and high concentrations of antioxidants were experimentally used,it is highly likely that the cardioprotective effect of antioxidants depends on their interaction not only with free radicals but also with other molecules.It has been demonstrated that the antioxidant N-2-mercaptopropionyl glycine or NDPH oxidase knockout abolished the cardioprotective effect of ischemic preconditioning.Consequently,there is evidence that ROS protect the heart against the I/R injury.

Identifying sex-specific injury predictors as a key factor in maintaining optimal physical activity levels

BACKGROUND Optimal physical activity is known to reduce cardiovascular,respiratory and endocrine system diseases and,as a consequence,improve quality of life.An important risk factor for reinjuries during normal exercise is the initial connective tissue pathology.The variety of clinical dysplastic manifestations significantly complicate the timely diagnosis of this comorbidity.AIM To establish pathognomonic sex-specific dysplasia phenotypes that indicate a particular sensitivity to physical exertion.METHODS The study involved 117 participants with recurrent musculoskeletal injuries that occurred during normal exercise.There were 67 women(57.26%)and 50 men(42.74%),which made it possible to compare the presence of the identified signs between sexes.A validated questionnaire was used to screen their connective tissue status.RESULTS Ranking the most commonly revealed dysplasia signs depending on their clinical significance made it possible to establish pathognomonic sex-specific phenotypes that indicated a particular susceptibility to injuries.Individualized programs of optimal physical activity are necessary for men with chest deformities,flat-valgus feet,dolichostenomelia,arachnodactylia,hemorrhoids,abdominal muscle diastasis and recurrent hernias.In women,special sensitivity to physical exertion was associated with a combination of signs such as asthenic body,joint hypermobility,overly soft auricles,thin hyperelastic skin,atrophic striae,telangiectasias and varicose veins.Of particular importance were universal signs such as gothic palate,scoliosis,kyphosis,leg deformities,temporomandibular joint crunching,and moderate to high myopia.CONCLUSION Participants’connective tissue condition should be considered when designing optimal physical activity programs.Identifying the established sex-specific dysplasia phenotypes will allow timely optimization of training loads,thus reducing the risk of injury.

Synthetic morphogenesis:why reverse engineering should be prioritized

Dear Editor,In morphogenesis,the sequential execution of tissue arrangement depends on a vast array of signaling proteins and cellular response.Several mechanisms of such response have been identified:apoptosis,proliferation,fusion,adhesion,sorting,transition,folding,migration and expansion.Different combinations of these responses lead to the formation of various anatomical structures[1,2].

Generation Algorithm of a Sort of P-Permutations

This paper design the P-permutation with cryptologic properties beyond certain bound based on BCH code and Goppa code, which is the generalization of the design method utilizing the maximum distance separable （MDS） code, and presents the novel algorithms to generate the P-permutations with the branch number no less than a preset low bound. The new generation algorithms provide a theoretical basis for automation design of the cryptosystem.

Enumeration of Generalized Linear Orthomorphism in the Vector Space F_q^n

The conception of orthomorphism has been generalized in this paper, and a counting formula on the generalized linear orthomorphism in the vector space over the Galois field with the arbitrary prime number p as the characteristic is obtained. Thus, the partial generation algorithm of generalized linear orthomorphism is achieved. The counting formula of the linear orthomorphism in the vector space over the finite field with characteristic 2 is the special case in our results. Furthermore, the generalized linear orthomorphism generated and discussed in this paper can gain the maximum branch number when they are designed as P-permutations.