AIM： Prior Helicobacterpylori （Hpylori infection has often been underestimated. These underestimations have misled physicians attempting to determine the significance between Hpyloriand certain gastrointestinal lesi...AIM： Prior Helicobacterpylori （Hpylori infection has often been underestimated. These underestimations have misled physicians attempting to determine the significance between Hpyloriand certain gastrointestinal lesions such as intestinal metaplasia, atrophic gastritis, and gastric cancer. Our study endeavored to debit past Hppylorinfections accurately, easily, and rapidly with the newly developed irnmunoblot kit, Helico Blot 2.1. METHODS： Thirty-three patients, including 25 H pylori infected and 8 uninfected cases, were enrolled in our study. All patients received consecutive gastroendoscopic examinations and ^3C-urea breath test （UBT） tests at 6- or 12-mo intervals for up to 4 years. Serum samples were obtained from each patient at the same time. Intragastric H pylori infection was confirmed in accordance with the gold standard. Twenty-five H pylori-infected patients received triple therapies after initial bacterial confirmation, and were successful in eradicating their infections. Serially obtained sera were tested by means of Helico Blot 2.1. RESULTS： Current infection marker detected by Helico Blot 2.1 was unreliable for representing ongoing Hpylori infection. Only 35 and 37 ku antibodies of H pylorihad significant seroconversion rates 1 year after having been cured. The seroposiUve rates of 116 ku （cytotoxin-associated antigen [CagA]） and Helico Blot 2.1 were nearly 100% during 4-year follow-up period. Both CagA antigen and Helico blot 2.1 could serve as indicators of long-term H pylori infection. CONCLUSION： Helico Blot 2.1 can detect past H pylori infections for up to 4 years, and is the best method to date for detecting previous long-term H pylori infection. 2005 The WJG Press and Elsevier Inc. All rights reserved.展开更多
基金Supported by Grants From the China American Petrochemical Co., Ltd. Foundation (CAPCO) and the National Science Council of the ROC (NSC-90-2314-B-037-044)
文摘AIM： Prior Helicobacterpylori （Hpylori infection has often been underestimated. These underestimations have misled physicians attempting to determine the significance between Hpyloriand certain gastrointestinal lesions such as intestinal metaplasia, atrophic gastritis, and gastric cancer. Our study endeavored to debit past Hppylorinfections accurately, easily, and rapidly with the newly developed irnmunoblot kit, Helico Blot 2.1. METHODS： Thirty-three patients, including 25 H pylori infected and 8 uninfected cases, were enrolled in our study. All patients received consecutive gastroendoscopic examinations and ^3C-urea breath test （UBT） tests at 6- or 12-mo intervals for up to 4 years. Serum samples were obtained from each patient at the same time. Intragastric H pylori infection was confirmed in accordance with the gold standard. Twenty-five H pylori-infected patients received triple therapies after initial bacterial confirmation, and were successful in eradicating their infections. Serially obtained sera were tested by means of Helico Blot 2.1. RESULTS： Current infection marker detected by Helico Blot 2.1 was unreliable for representing ongoing Hpylori infection. Only 35 and 37 ku antibodies of H pylorihad significant seroconversion rates 1 year after having been cured. The seroposiUve rates of 116 ku （cytotoxin-associated antigen [CagA]） and Helico Blot 2.1 were nearly 100% during 4-year follow-up period. Both CagA antigen and Helico blot 2.1 could serve as indicators of long-term H pylori infection. CONCLUSION： Helico Blot 2.1 can detect past H pylori infections for up to 4 years, and is the best method to date for detecting previous long-term H pylori infection. 2005 The WJG Press and Elsevier Inc. All rights reserved.
