Cloning and Expression of Bile Salt Hydrolase Gene from Lactobacillus plantarum M1-UVS29

We cloned and expressed bile salt hydrolase gene of Lactobacillus plantarum M1-UVS29 in Lactococcus lactis NZ9000 successfully. Gene-specific primers for amplification of L. plantarum bsh were designed by using sequence which availabled from Gen Bank. The production of PCR amplicon was confirmed by sequencing and cloned into pMD18-T vector, and then recombined into expression vector p NZ8148 and yielding vector p NZ8148-BSH. p NZ8148-BSH was transferred into Lactococcus lactis NZ9000. Sequencing indicated that the cloned bsh fragment contained 995 nucleotides, and shared 99.3% sequence homology with bsh gene from L. plantarum MBUL10. Cloned bsh fragment was successfully transduced into NICE expression system and confirmed by PCR and restriction digest. Recombinant BSH protein was analyzed by SDS-PAGE. The molecular weight of BSH protein was approximately 37 ku. Activity of the expressed protein was 0.77 μmol · min-1. The successfully expressed proteins by genetic engineering technology made the function of lactic acid bacteria be abundant and laid the foundation for further researches into cholesterol-lowering lactic acid bacterium food and probiotics.

Influence of dietary isoflavone intake on gastrointestinal symptoms in ulcerative colitis individuals in remission

AIM To analyse the association between isoflavone intake and ulcerative colitis motility symptoms in individuals in remission. METHODS Cross-sectional study was conducted in a group of ulcerative colitis remission individuals, in subgroups characterised by various intestinal motility and functioning characteristics(abdominal pain, flatulence, constipations, tenesmus). Total of 56 individuals with ulcerative colitis in remission(19 males and 37 females) were recruited for the study. Assessment of diet was based on self-reported data from each patient's dietary records taken over a period of three typical, random days(2 weekdays and 1 d of the weekend). The daily isoflavone intake(daidzein, genistein, glycitein and total isoflavones) and daily isoflavone intake per 1000 kcal of diet were assessed.RESULTS No correlations between isoflavone intake levels and number of bowel movements per day were observed both in the case of intake and intake per 1000 kcal of diet. In the group of individuals declaring lack of abdominal pain, the higher intakes of daidzein(P = 0.0075), daidzein per 1000 kcal of diet(P = 0.0358) and total isoflavone(P = 0.0358) were stated, than in the group of individuals declaring abdominal pain. In the group of individuals declaring lack of constipations, the lower intakes of glycitein(P = 0.0213) and glycitein per 1000 kcal of diet(P = 0.0213) were stated, than in the group of individuals declaring presence of constipations. No differences were observed in isoflavone intake between groups of ulcerative colitis individuals declaring lack of flatulence and declaring presence of flatulence, as well as between groups declaring lack of tenesmus and declaring presence of tenesmus.CONCLUSION The moderate dietary isoflavone intake may be beneficial for individuals with ulcerative colitis in remission, however, before including it into recommendations, further prospective studies are needed.

Security Threats to Business Information Systems Using NFC Read/Write Mode

Radio Frequency IDentification(RFID)and related technologies such as Near Field Communication(NFC)are becoming essential in industrial contexts thanks to their ability to perform contactless data exchange,either device-to-device or tag-to-device.One of the three main operation modes of NFC,called read/write mode,makes use of the latter type of interaction.It is extensively used in business information systems that make use of NFC tags to provide the end-user with augmented information in one of several available NFC data exchange formats,such as plain text,simple URLs or enriched URLs.Using a wide variety of physical form factors,NFC-compatible tags(wireless transponders)are currently available in many locations with applications going from smart posters,contactless tokens,tap-and-go payments or transport ticketing to automated device configuration,patient identification at hospitals or inventory management within supply chains.Most of these applications handle sensitive processes or data.This paper proposes a complete security threat model for the read/write operation mode of NFC used in Next Generation Industrial IoT(Nx-IIoT)contexts.This model,based on a wellknown methodology,STRIDE,allows developers and users to identify NFC applications vulnerabilities or weaknesses,analyze potential threats,propose risk management strategies,and design mitigation mechanisms to mention only some significant examples.

A Fast Fourth-Order Method for 3D Helmholtz Equation with Neumann Boundary

We present fast fourth-order finite difference scheme for 3D Helmholtz equation with Neumann boundary condition. We employ the discrete Fourier transform operator and divide the problem into some independent subproblems. By means of the Gaussian elimination in the vertical direction, the problem is reduced into a small system on the top layer of the domain. The procedure for solving the numerical solutions is accelerated by the sparsity of Fourier operator under the space complexity of O(M3). Furthermore, the method makes it possible to solve the 3D Helmholtz equation with large grid number. The accuracy and efficiency of the method are validated by two test examples which have exact solutions.

Mathematical Modeling Algorithms for Creating New Materials with Desired Properties Using Nano-Hierarchical Structures

In the enormous and still poorly mastered gap between the macro level, where well developed continuum theories of continuous media and engineering methods of calculation and design operate, and atomic, subordinate to the laws of quantum mechanics, there is an extensive meso-hierarchical level of the structure of matter. At this level unprecedented previously products and technologies can be artificially created. Nano technology is a qualitatively new strategy in technology: it creates objects in exactly the opposite way—large objects are created from small ones [1]. We have developed a new method for modeling acoustic monitoring of a layered-block elastic medium with several inclusions of various physical and mechanical hierarchical structures [2]. An iterative process is developed for solving the direct problem for the case of three hierarchical inclusions of l, m, s-th ranks based on the use of 2D integro-differential equations. The degree of hierarchy of inclusions is determined by the values of their ranks, which may be different, while the first rank is associated with the atomic structure, the following ranks are associated with increasing geometric sizes, which contain inclusions of lower ranks and sizes. Hierarchical inclusions are located in different layers one above the other: the upper one is abnormally plastic, the second is abnormally elastic and the third is abnormally dense. The degree of filling with inclusions of each rank for all three hierarchical inclusions is different. Modeling is carried out from smaller sizes to large inclusions;as a result, it becomes possible to determine the necessary parameters of the formed material from acoustic monitoring data.

A Recognized Value System among Nurses Working in Poland

Aim of this work was to present a study indicating the recognized system of values of nursing units in healthcare entities in Poland. Professionally active nurses (249) employed in medicinal entities were subjects in the study. A random selection of individuals was applied in the study, and the participation in the research was declared by respondents consent to participate in it. A survey was used as the main form of measurement, and it was divided into direct and indirect survey. A standardized Rokeach’s scale of values [1] [2] consisting of two collections, each of which included 18 single values, was used. They were ranked by tested individuals according to the hierarchy of their values which allowed for recognizing which are the most important for them. Kruskal-Wallis’s test was applied in the study. Results: The social position is the most important value for nurses working in the district medical entities. For those working in the regional medical units, the most appreciated value is being ambitious, whereas in the clinical self-esteem. The socio-demographic data such as age, length of service, levels of education differentiate assessments of each recognized values in the group of nurses. The women between 31 and 40 consider being ambitious as the most important value, between 41 and 50 responsibility and between 20 and 30 self-esteem;responsibility is the most appreciated value for people with 21 - 25 years of experience because the older and more experienced the nurse is, the stronger her sense of responsibility becomes. A social position was appreciated the most by the nurses holding a nursing degree. Conclusions: There have been significant differences in terms of accepted values between nurses working in the district, provincial and clinical medical entities. Socio-demographic data such as age, length of service, education levels differentiate assessment of each recognized value in the group of nurses.

The Role of Social Media in Providing Crisis Information in China:A Critical Evaluation of the Tianjin Fire Incident

This paper focusses on the information asymmetry in crisis news after a serious incident in Tianjin, China, in 2015. The incident caused enormous damage and resulted in societal unrest because of the lack of reliable information from the formal media channels. Social media — micro blogs —played a major role in reporting on crisis situations. We divided netizens(i.e., the citizens of the net)into high and low types according to their information-critical level to the crisis news. The data shows information deterioration on the crisis news, related to the netizens' information-critical level. For the traditional media there is the opportunity to use information quality distortion to make more marginal profits. This is possible only if the citizens' information stays under a certain quality level. The result is overprovision of low quality news and high quality news driven out of the market, whereupon adverse selection(i.e., a lack of symmetric information) appears. However, by adopting a process view, we found self-correcting mechanism(i.e., dying out of rumors) of the social media communities in China.We provided a agent-base model and simulation to show that the more media exist in the market, the faster speed of the information deterioration, but also the capacity to ‘discuss' rumors.

Where there is light,there is dark:a dual process model of high-performance work systems in the eyes of employees

High-performance work systems have been widely adopted in the workplace. Previous research on high-performance work systems debated whether the generated effects are mutual gains of conflicting outcomes for employers and employees.Drawing on the job demands and resources model,this conceptual study proposes that high-performance work systems can be both beneficial and harmful by eliciting distinct perceptions in employees.Specifically,perceptions of job resources are the positive and perceptions of job demands are the negative mechanism whereby high-performance work systems affect employee job performance.This research further proposes that servant leadership strengthens the positive impact of high-performance work systems,whereas directive leadership strengthens the negative impact.Overall,this conceptual research provides new insights into the research on high-performance work systems.

How do work-family balance practices affect work-family conflict？ The differential roles of work stress

这份报纸调查在工作家庭平衡惯例和工作家庭冲突的雇员感觉之间的关系。它作为主考检验挑战应力和阻碍者应力的角色。基于在北京从 841 个公务员收集的调查数据，我们发现察觉的工作家庭平衡惯例可以减少工作家庭冲突，当挑战和阻碍者工作压力是时断然与工作家庭有关冲突。另外，挑战和阻碍者压力差别节制了在察觉的工作家庭惯例和工作家庭冲突之间的关系。当挑战应力当时高时，工作家庭平衡惯例将减少工作家庭冲突。在高阻碍者应力下面，然而，工作家庭平衡惯例将服务减少工作家庭冲突更少。工作家庭平衡惯例(工作灵活性，和雇员和家庭好照顾) 的 configurational 尺寸的更多的详细分析也被测试。这研究为未来研究提供另外的卓见进工作家庭接口和提议想法的管理。

组织研究中的网络范式:文献综述和一个分类框架

在这篇文章中,我们综述并分析了组织研究中新兴的网络范式。首先,我们围绕着现有的研究领域组织文献,对近期研究成果进行了常规的综述。然后,我们对这些研究进行分析,建立了一组区别网络研究的维度,包括因果关系的方向、分析的层次、解释目标和解释机制。在此基础上,我们用后两个维度建立了一个2×2的表格,把网络结果研究交叉分类成4个典型:结构性社会资本,资源的社会可得性,蔓延和环境塑造。我们注意到,与网络领域的传统研究相比,越来越多的研究更关注于对主体的理解。

Transformative Innovation Policy:A Case-Study from China

Science,technology and innovation policy has been presented as developing through three"frames":innovation for growth;national systems of innovation;and transformative change(Schot and Steinmueller,2018).In this paper,we focus on the third frame by exploring the development of transformative innovation policy in China.More specifically,we present a case analysis of the Beautiful China Initiative(BCI).Within this context,we analyse participating actors,activities,and modes of innovation.In conclusion,we discuss three general challenges of transformative innovation policy at a national and international level.The first challenge is coordination and interaction between governmental agencies and other actors with different responsibilities and jurisdictions.Second,there is a major set of issues related to"development",which must encompass economic development but also societal and environmental development.Third,BCI fits with a broad understanding of the innovation process,including elements which are not directly commercial in orientation.Whether this broader approach can be maintained as BCI develops from its still-early stage into full development will represent a significant challenge.Finally in this paper,we consider the implications of our discussion for future research and practice,and offer three reflections.In particular,we emphasise the importance of scholars’participation across the social sciences rather than leaving this multi-faceted area only to innovation researchers.