Studies of CTLA4Ig in acute rejection of pancreas transplantation in rats

Objective： To investigate the protective effect of CTLA4Ig in rejection of pancreaticoduodenal transplantation model of rat. Methods ： Pancreaticoduodenal transplantion models were established from the donor F344 rats to the Lewis recipients. The models were divided into 2 groups： Group A and B with 12 rats in each group.2 days after transplantation, reciepients in group A were treated with i.p. injection of sailine, and those in group B CTLA41 were injected（200μg）. On day 1,4,7, 10 after transplantation, the grafts were harvested for histopathological examination. On day 4 after transplantation, the CD4^＋CD25^＋ T cells in the grafts were detected by Flow Cytometry. Results： Compared with group A： the degree of the rejection of grafts in group B was lower. The number of CD4^CD25^＋ T cells of graft was （7.91±1.26）% in group A and （13.81±1.71）% in group B, which had significant difference （P〈0.01）. Conclusion： CTLA4Ig could inhibit T cell costimulatory pathway, prevent acute rejection, which might be mediated bv increasing the number of CD4^＋CD25^＋ regulatory T cells.

Detection of K-ras Gene Point Mutation＇s Style in Human Pancreatic Cancer Cell Line PANC-1 by PCR-SSP

Objective： To detect the style of K-ras gene point mutation in human pancreatic cancer cell line PANC-1 and decide the bp sequence of Ras target position interfered by RNA. Methods： Three kinds of special sequence primers （SSP） for polymerase chain reaction （PCR） with regard to the mutation styles （OAT, COT and GOT） at codon 12 of K-ras were used to study the human pancreatic cancer cell line PANC-1. The amplification products were studied with polyacrylamine gel electrophoresis to detect the style of point mutation. Results： The style of K-ras gene point mutation at codon 12 was OAT in human pancreatic cancer cell line. Conclusion： PCR-SSP is rapid, convenient and high specific. The results provide a basis for further gene therapy by RNA interference for pancreatic cancer.

Assessment of prophylactic antibiotics administration for acute pancreatitis:a meta-analysis of randomized controlled trials

Background:Recent evidence has shown that prophylactic antibiotic treatment in patients with acute pancreatitis is not associated with a significant decrease in mortality or morbidity.The use and efficacy of prophylactic antibiotic treatment in acute pancreatitis remain controversial.This meta-analysis was conducted to assess whether antibiotic prophylaxis is beneficial in patients with acute pancreatitis.Methods:We searched randomized controlled trials(RCTs)of prophylactic use of antibiotics using Medline(PubMed),Embase,the Cochrane Library,and Web of Science.The data were analyzed using Review Manager 5.3 software.We performed pooled analyses for infected pancreatic necrosis,mortality,surgical intervention,and non-pancreatic infection.Odds ratios(ORs)from each trial were pooled using a random or fixed effects model,depending on the heterogeneity of the included studies.Sub-group analysis or sensitivity analysis was conducted to explore potential sources of heterogeneity,when necessary.Results:Totally,11 RCTs involving 747 participants were included,with an intervention group(prophylactic use of antibiotics,n=376)and control group(n=371).No significant differences were found regarding antibiotic prophylaxis with respect to incidence of infected pancreatic necrosis(OR,0.74;95%confidence interval[CI],0.50–1.09;P=0.13),surgical intervention(OR,0.92;95%CI,0.62–1.38;P=0.70),and morality(OR,0.71;95%CI,0.44–1.15;P=0.16).However,antibiotic prophylaxis was associated with a statistically significant reduction in the incidence of non-pancreatic infection(OR,0.59;95%CI,0.42–0.84;P=0.004).Conclusions:Prophylactic antibiotics can reduce the incidence of non-pancreatic infection in patients with AP.

Comparative analysis of duodenum-preserving pancreatic head resection and pancreaticoduodenectomy

There is controversy regarding the perioperative complications,safety,and long-term quality of life for duodenum-preserving pancreatic head resection(DPPHR)and pancreaticoduodenectomy(PD).We performed a comparative analysis of DPPHR and PD in the treatment of benign and low-grade malignant diseases of the pancreatic head.The study was approved by the Institutional Review Board of the Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University(No.K202001-14),and was in accordance with the Declaration of Helsinki.Because this was a retrospective study and the data analysis was performed anonymously,this study was exempt from informed consent from patients.