The human immunodeficiency virus （HIV） lentiviral vector is an ideal vector for gene therapy. In the present study, the wild-type HIV-1 genome was segregated into four plasmids, and an optimized novel HIV-1 lentivir...The human immunodeficiency virus （HIV） lentiviral vector is an ideal vector for gene therapy. In the present study, the wild-type HIV-1 genome was segregated into four plasmids, and an optimized novel HIV-1 lentiviral vector containing green fluorescent protein and vesicular stomatitis virus G pseudo-capsule was constructed. The plasmids were pHR-CMV-EGFP, pCMVΔ8.9, pRSV-Rev, pCMV-VSV-G. The four plasmid system was co-transfected into 293T cells, and green fluorescent protein expression was observed. The present study obtained lentiviral particles by high-speed centrifugation, and the lentiviral particle titer was 4 × 108 TU/mL after centrifugation. Thus, an optimized novel HIV-1 lentiviral vector was successfully constructed.展开更多
Background Previous research indicated that the development of diabetic retinopathy ( DR) is closely related to the excessive expression of growth factors. This paper was to study the relationship of DR with vascular ...Background Previous research indicated that the development of diabetic retinopathy ( DR) is closely related to the excessive expression of growth factors. This paper was to study the relationship of DR with vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and the retinal vascular pathological change.Methods Fifty-five Wistar rats, weighing 100 -200 g, were selected and randomly divided into four groups: control group (no streptozocin injection, n =10), M1 group (streptozocin induced diabetes for 1 month, n =15), M3 group (streptozocin induced diabetes for 3 months, n =15), and M5 group (streptozocin induced diabetes for 5 months, n =15). In situ hybridization and immunohistochemistry were used to investigate the expressions of bFGF and VEGF on retinal vascular, and retinal vessels were observed by transmission electron microscope.Results There was no difference in the number of pericytes between M1 and control group (P > 0. 05), but the number of pericytes decreased obviously in M3 and M5 groups compared with the control group (P<0. 01, P<0. 001, respectively). Capillary embolization and non-cell capillary were seen in M5 group. Positive expression of VEGF was found in M5 group using in situ hybridization and immunohistochemistry respectively. Positive expression of bFGF could be seen in M3(78%) and M5 group (89%). Most remarkable changes of vessles were observed in M5 group including fragmental thickness, split of basement membrane, swelling and distortion of endothelial cells.Conclusions In retinal vascular of the streptozocin (STZ) rats, there shows the expression of bFGF at the third month and that of VEGF at the fifth month.展开更多
基金the National Natural Science Foundation of China, No. 30770755
文摘The human immunodeficiency virus （HIV） lentiviral vector is an ideal vector for gene therapy. In the present study, the wild-type HIV-1 genome was segregated into four plasmids, and an optimized novel HIV-1 lentiviral vector containing green fluorescent protein and vesicular stomatitis virus G pseudo-capsule was constructed. The plasmids were pHR-CMV-EGFP, pCMVΔ8.9, pRSV-Rev, pCMV-VSV-G. The four plasmid system was co-transfected into 293T cells, and green fluorescent protein expression was observed. The present study obtained lentiviral particles by high-speed centrifugation, and the lentiviral particle titer was 4 × 108 TU/mL after centrifugation. Thus, an optimized novel HIV-1 lentiviral vector was successfully constructed.
基金This study is supported by grants from Jilin Province Scientific Foundation (No. 990575-5) Changchun Science Committee (No.01-106530) Jilin University (No. 200109).
文摘Background Previous research indicated that the development of diabetic retinopathy ( DR) is closely related to the excessive expression of growth factors. This paper was to study the relationship of DR with vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and the retinal vascular pathological change.Methods Fifty-five Wistar rats, weighing 100 -200 g, were selected and randomly divided into four groups: control group (no streptozocin injection, n =10), M1 group (streptozocin induced diabetes for 1 month, n =15), M3 group (streptozocin induced diabetes for 3 months, n =15), and M5 group (streptozocin induced diabetes for 5 months, n =15). In situ hybridization and immunohistochemistry were used to investigate the expressions of bFGF and VEGF on retinal vascular, and retinal vessels were observed by transmission electron microscope.Results There was no difference in the number of pericytes between M1 and control group (P > 0. 05), but the number of pericytes decreased obviously in M3 and M5 groups compared with the control group (P<0. 01, P<0. 001, respectively). Capillary embolization and non-cell capillary were seen in M5 group. Positive expression of VEGF was found in M5 group using in situ hybridization and immunohistochemistry respectively. Positive expression of bFGF could be seen in M3(78%) and M5 group (89%). Most remarkable changes of vessles were observed in M5 group including fragmental thickness, split of basement membrane, swelling and distortion of endothelial cells.Conclusions In retinal vascular of the streptozocin (STZ) rats, there shows the expression of bFGF at the third month and that of VEGF at the fifth month.
