Objective We examined alterations in the expression of tumorigenesis‐related genes in the pituitary gland of rats exposed to electromagnetic pulses （EMP）.Methods The global gene expression profiles of the pituitary...Objective We examined alterations in the expression of tumorigenesis‐related genes in the pituitary gland of rats exposed to electromagnetic pulses （EMP）.Methods The global gene expression profiles of the pituitary gland in EMP‐exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis‐related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real‐time‐PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome‐wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP‐exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis‐related genes in the pituitary gland.These tumorigenesis‐related genes are potentially involved in the development of pituitary gland tumors in rats.展开更多
基金supported by the Research Fund of the National Natural Science Foundation of China (No: 60871068 30970670)
文摘Objective We examined alterations in the expression of tumorigenesis‐related genes in the pituitary gland of rats exposed to electromagnetic pulses （EMP）.Methods The global gene expression profiles of the pituitary gland in EMP‐exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis‐related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real‐time‐PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome‐wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP‐exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis‐related genes in the pituitary gland.These tumorigenesis‐related genes are potentially involved in the development of pituitary gland tumors in rats.
